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Multiplex RT-PCR assay for the detection of major fusion transcripts in Taiwanese children with B-lineage acute lymphoblastic leukemia.
Med Pediatr Oncol. 2002 Jul; 39(1):12-7.MP

Abstract

BACKGROUND

The classification of B-lineage acute lymphoblastic leukemia (ALL) by specific chromosomal translocations may have prognostic implications. Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemic cells. In general, fusion transcripts are determined individually, a process which is labor intensive in order to detect all major fusion transcripts.

PROCEDURE

We use a multiplex RT-PCR assay to detect both the CML- and ALL-type BCR-ABL transcripts of the t(9;22), all described variants of the E2A-PBX1 transcripts of t(1;19), the MLL-AF4 transcripts of t(4;11), and all described variants of TEL-AML1 (also termed ETV6-CBFA2) of the cryptic t(12;21) in 165 leukemic samples at diagnosis.

RESULTS

The study yielded a completely concordant result with those obtained by the individual RT-PCR assay. In this cohort of Taiwan children, the relative frequencies of the four translocations of B-lineage ALL were as following: 6% with ALL-type t(9;22)/BCR-ABL, 7% t(1;19)/E2A-PBX1, 3% t(4;11)/MLL-AF4, and 18% t(12;21)/TEL-AML1, comparable to those in the Western countries.

CONCLUSION

Multiplex RT-PCR assay is an efficient, sensitive, accurate, and cost-effective diagnostic tool, which will likely improve our ability in accurately and rapidly risk-stratifying children with ALL.

Authors+Show Affiliations

Division of Pediatric Hematology-Oncology, Mackay Memorial Hospital, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12116073

Citation

Liang, Der-Cherng, et al. "Multiplex RT-PCR Assay for the Detection of Major Fusion Transcripts in Taiwanese Children With B-lineage Acute Lymphoblastic Leukemia." Medical and Pediatric Oncology, vol. 39, no. 1, 2002, pp. 12-7.
Liang DC, Shih LY, Yang CP, et al. Multiplex RT-PCR assay for the detection of major fusion transcripts in Taiwanese children with B-lineage acute lymphoblastic leukemia. Med Pediatr Oncol. 2002;39(1):12-7.
Liang, D. C., Shih, L. Y., Yang, C. P., Hung, I. J., Chen, S. H., Jaing, T. H., Liu, H. C., & Chang, W. H. (2002). Multiplex RT-PCR assay for the detection of major fusion transcripts in Taiwanese children with B-lineage acute lymphoblastic leukemia. Medical and Pediatric Oncology, 39(1), 12-7.
Liang DC, et al. Multiplex RT-PCR Assay for the Detection of Major Fusion Transcripts in Taiwanese Children With B-lineage Acute Lymphoblastic Leukemia. Med Pediatr Oncol. 2002;39(1):12-7. PubMed PMID: 12116073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex RT-PCR assay for the detection of major fusion transcripts in Taiwanese children with B-lineage acute lymphoblastic leukemia. AU - Liang,Der-Cherng, AU - Shih,Lee-Yung, AU - Yang,Chao-Ping, AU - Hung,Iou-Jih, AU - Chen,Shu-Huey, AU - Jaing,Tang-Her, AU - Liu,Hsi-Che, AU - Chang,Wan-Hui, PY - 2002/7/13/pubmed PY - 2002/8/7/medline PY - 2002/7/13/entrez SP - 12 EP - 7 JF - Medical and pediatric oncology JO - Med Pediatr Oncol VL - 39 IS - 1 N2 - BACKGROUND: The classification of B-lineage acute lymphoblastic leukemia (ALL) by specific chromosomal translocations may have prognostic implications. Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemic cells. In general, fusion transcripts are determined individually, a process which is labor intensive in order to detect all major fusion transcripts. PROCEDURE: We use a multiplex RT-PCR assay to detect both the CML- and ALL-type BCR-ABL transcripts of the t(9;22), all described variants of the E2A-PBX1 transcripts of t(1;19), the MLL-AF4 transcripts of t(4;11), and all described variants of TEL-AML1 (also termed ETV6-CBFA2) of the cryptic t(12;21) in 165 leukemic samples at diagnosis. RESULTS: The study yielded a completely concordant result with those obtained by the individual RT-PCR assay. In this cohort of Taiwan children, the relative frequencies of the four translocations of B-lineage ALL were as following: 6% with ALL-type t(9;22)/BCR-ABL, 7% t(1;19)/E2A-PBX1, 3% t(4;11)/MLL-AF4, and 18% t(12;21)/TEL-AML1, comparable to those in the Western countries. CONCLUSION: Multiplex RT-PCR assay is an efficient, sensitive, accurate, and cost-effective diagnostic tool, which will likely improve our ability in accurately and rapidly risk-stratifying children with ALL. SN - 0098-1532 UR - https://www.unboundmedicine.com/medline/citation/12116073/Multiplex_RT_PCR_assay_for_the_detection_of_major_fusion_transcripts_in_Taiwanese_children_with_B_lineage_acute_lymphoblastic_leukemia_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0098-1532&date=2002&volume=39&issue=1&spage=12 DB - PRIME DP - Unbound Medicine ER -