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Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable Mycobacterium leprae in normal and immunosuppressed Swiss white mice.
Int J Lepr Other Mycobact Dis. 2002 Mar; 70(1):25-33.IJ

Abstract

Freshly harvested M. leprae were microinjected into the sciatic nerves of nonimmunosuppressed (non-TR) and immunosuppressed (TR) mice using the technique described by Wisniewski and Bloom. The lesions thus induced, on bypassing the blood-nerve barrier, were biopsied at regular intervals beginning 24 hr and followed up to one year. The fate of M. leprae and the ensuing inflammation and nerve damage were studied using light and electron microscopy. The lesions in both non-TR and TR mice at 24 hr showed an influx of polymorphonuclear leukocytes and an increase in mast cells. The influx and peaking of lymphocytes were delayed by two weeks and 6 weeks, respectively, in TR mice, but the density of lymphocytes at the peak intervals was comparable in both. The plasma cells denoting the humoral response were seen in both, but there was a delay of 3 weeks in non-TR mice. The lesions in non-TR mice showed differentiation of macrophages into epithelioid cells and the formation of giant cells depicting borderline tuberculoid leprosy (BT), Whereas in TR mice, the macrophages showed foamy cytoplasmic changes depicting borderline lepromatous leprosy (BL). Other significant observations common to both non-TR and TR mice were: a) The lesions remained highly localized and showed signs of regression at the 6th and the 12th month intervals. b) The characteristic segmental demyelination and some attempt at remyelination were seen at the site. c) The influx of lymphocytes concorded well with demyelination. d) Bacteria were only seen in the macrophages and never in the Schwann cells or endothelial cells. e) Bacteria persisted in the macrophages, but appeared progressively degenerate at the 6th and 12th post-inoculation months, suggesting loss of viability. The study shows that there was a very effective containment of the infection and that the Schwann cells were resistant to M. leprae infection in the neural milieu. Nerve damage and Schwann cell bacillation do not go hand-in-hand.

Authors+Show Affiliations

Foundation for Medical Research, 84-A R.G. Thadani Marg, Worli, Bombay 400 018, India. frchbom@bom2.vsnl.net.inNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12120037

Citation

Shetty, Vanaja Prabhakar, and Noshir Hormusji Antia. "Light and Ultrastructural Study of Sciatic Nerve Lesions Induced Using Intraneural Injection of Viable Mycobacterium Leprae in Normal and Immunosuppressed Swiss White Mice." International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, vol. 70, no. 1, 2002, pp. 25-33.
Shetty VP, Antia NH. Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable Mycobacterium leprae in normal and immunosuppressed Swiss white mice. Int J Lepr Other Mycobact Dis. 2002;70(1):25-33.
Shetty, V. P., & Antia, N. H. (2002). Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable Mycobacterium leprae in normal and immunosuppressed Swiss white mice. International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, 70(1), 25-33.
Shetty VP, Antia NH. Light and Ultrastructural Study of Sciatic Nerve Lesions Induced Using Intraneural Injection of Viable Mycobacterium Leprae in Normal and Immunosuppressed Swiss White Mice. Int J Lepr Other Mycobact Dis. 2002;70(1):25-33. PubMed PMID: 12120037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable Mycobacterium leprae in normal and immunosuppressed Swiss white mice. AU - Shetty,Vanaja Prabhakar, AU - Antia,Noshir Hormusji, PY - 2002/7/18/pubmed PY - 2002/8/1/medline PY - 2002/7/18/entrez SP - 25 EP - 33 JF - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association JO - Int J Lepr Other Mycobact Dis VL - 70 IS - 1 N2 - Freshly harvested M. leprae were microinjected into the sciatic nerves of nonimmunosuppressed (non-TR) and immunosuppressed (TR) mice using the technique described by Wisniewski and Bloom. The lesions thus induced, on bypassing the blood-nerve barrier, were biopsied at regular intervals beginning 24 hr and followed up to one year. The fate of M. leprae and the ensuing inflammation and nerve damage were studied using light and electron microscopy. The lesions in both non-TR and TR mice at 24 hr showed an influx of polymorphonuclear leukocytes and an increase in mast cells. The influx and peaking of lymphocytes were delayed by two weeks and 6 weeks, respectively, in TR mice, but the density of lymphocytes at the peak intervals was comparable in both. The plasma cells denoting the humoral response were seen in both, but there was a delay of 3 weeks in non-TR mice. The lesions in non-TR mice showed differentiation of macrophages into epithelioid cells and the formation of giant cells depicting borderline tuberculoid leprosy (BT), Whereas in TR mice, the macrophages showed foamy cytoplasmic changes depicting borderline lepromatous leprosy (BL). Other significant observations common to both non-TR and TR mice were: a) The lesions remained highly localized and showed signs of regression at the 6th and the 12th month intervals. b) The characteristic segmental demyelination and some attempt at remyelination were seen at the site. c) The influx of lymphocytes concorded well with demyelination. d) Bacteria were only seen in the macrophages and never in the Schwann cells or endothelial cells. e) Bacteria persisted in the macrophages, but appeared progressively degenerate at the 6th and 12th post-inoculation months, suggesting loss of viability. The study shows that there was a very effective containment of the infection and that the Schwann cells were resistant to M. leprae infection in the neural milieu. Nerve damage and Schwann cell bacillation do not go hand-in-hand. SN - 0148-916X UR - https://www.unboundmedicine.com/medline/citation/12120037/Light_and_ultrastructural_study_of_sciatic_nerve_lesions_induced_using_intraneural_injection_of_viable_Mycobacterium_leprae_in_normal_and_immunosuppressed_Swiss_white_mice_ L2 - https://medlineplus.gov/mycobacterialinfections.html DB - PRIME DP - Unbound Medicine ER -