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The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site.
J Gen Virol. 2002 Aug; 83(Pt 8):2007-2014.JG

Abstract

The ZEBRA protein encoded by the Epstein-Barr virus (EBV) genome activates a switch from the latent to the lytic gene expression programme of the virus. ZEBRA, a member of the basic leucine zipper family of DNA-binding proteins, is a transcriptional activator capable of inducing expression from several virus lytic cycle promoters by binding to activator protein 1 (AP-1)-like sites. The Epstein-Barr virus BamHI F promoter, Fp, was for some time believed to initiate EBNA1-specific transcription in EBV-transformed latent cells. More recent data, however, show that Fp is an early lytic promoter and that the dominant EBNA1 gene promoter in latent cells is Qp, located about 200 bp downstream of Fp. In the present investigation we confirm that Fp displays the characteristics of a lytic promoter. Fp is downregulated in latently EBV-infected cells, both in the endogenous virus genome and in reporter plasmids that carry Fp regulatory sequences upstream of position -136 and down to +10 relative to the Fp transcription start site (+1), and is activated on induction of the virus lytic cycle. We show that the repression of Fp in latent stages of infection can be abolished by ZEBRA, and demonstrate that ZEBRA activates Fp through a direct interaction with an AP-1-like site at position -52/-46 in the promoter-proximal Fp region.

Authors+Show Affiliations

Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, S-413 45 Gothenburg, Sweden1.Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, S-413 45 Gothenburg, Sweden1.Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, S-413 45 Gothenburg, Sweden1.Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden2.Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden2.Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden2.Cellular and Molecular Tumor Pathology, Cancer Centrum Karolinska, CCK R8:04, Karolinska Hospital, S-171 76 Stockholm, Sweden3.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12124465

Citation

Zetterberg, Henrik, et al. "The Epstein-Barr Virus ZEBRA Protein Activates Transcription From the Early Lytic F Promoter By Binding to a Promoter-proximal AP-1-like Site." The Journal of General Virology, vol. 83, no. Pt 8, 2002, pp. 2007-2014.
Zetterberg H, Jansson A, Rymo L, et al. The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site. J Gen Virol. 2002;83(Pt 8):2007-2014.
Zetterberg, H., Jansson, A., Rymo, L., Chen, F., Karlsson, A., Klein, G., & Brodin, B. (2002). The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site. The Journal of General Virology, 83(Pt 8), 2007-2014. https://doi.org/10.1099/0022-1317-83-8-2007
Zetterberg H, et al. The Epstein-Barr Virus ZEBRA Protein Activates Transcription From the Early Lytic F Promoter By Binding to a Promoter-proximal AP-1-like Site. J Gen Virol. 2002;83(Pt 8):2007-2014. PubMed PMID: 12124465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site. AU - Zetterberg,Henrik, AU - Jansson,Ann, AU - Rymo,Lars, AU - Chen,Fu, AU - Karlsson,Ann, AU - Klein,Georg, AU - Brodin,Bertha, PY - 2002/7/19/pubmed PY - 2002/8/30/medline PY - 2002/7/19/entrez SP - 2007 EP - 2014 JF - The Journal of general virology JO - J Gen Virol VL - 83 IS - Pt 8 N2 - The ZEBRA protein encoded by the Epstein-Barr virus (EBV) genome activates a switch from the latent to the lytic gene expression programme of the virus. ZEBRA, a member of the basic leucine zipper family of DNA-binding proteins, is a transcriptional activator capable of inducing expression from several virus lytic cycle promoters by binding to activator protein 1 (AP-1)-like sites. The Epstein-Barr virus BamHI F promoter, Fp, was for some time believed to initiate EBNA1-specific transcription in EBV-transformed latent cells. More recent data, however, show that Fp is an early lytic promoter and that the dominant EBNA1 gene promoter in latent cells is Qp, located about 200 bp downstream of Fp. In the present investigation we confirm that Fp displays the characteristics of a lytic promoter. Fp is downregulated in latently EBV-infected cells, both in the endogenous virus genome and in reporter plasmids that carry Fp regulatory sequences upstream of position -136 and down to +10 relative to the Fp transcription start site (+1), and is activated on induction of the virus lytic cycle. We show that the repression of Fp in latent stages of infection can be abolished by ZEBRA, and demonstrate that ZEBRA activates Fp through a direct interaction with an AP-1-like site at position -52/-46 in the promoter-proximal Fp region. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/12124465/The_Epstein_Barr_virus_ZEBRA_protein_activates_transcription_from_the_early_lytic_F_promoter_by_binding_to_a_promoter_proximal_AP_1_like_site_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/0022-1317-83-8-2007 DB - PRIME DP - Unbound Medicine ER -