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Relative contribution of NF-kappaB and AP-1 in the modulation by curcumin and pyrrolidine dithiocarbamate of the UVB-induced cytokine expression by keratinocytes.
Cytokine. 2002 May 07; 18(3):168-77.C

Abstract

Following ultraviolet B treatment, expression of tumour necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 by NCTC 2544 keratinocyte cell line was significantly enhanced both at the mRNA and protein level. The UVB also increased the IL-10 steady-state mRNAs level. Radiation-induced cytokine overexpression was accompanied by NF-kappaB and AP-1 transcription factors activation as assessed by electrophoretic mobility shift assays. To investigate in keratinocytes the relative contributions of those transcription factors on UVB-mediated cytokine induction, cell cultures were supplemented with curcumin and pyrrolidine dithiocarbamate (PDTC), agents known to modulate NF-kappaB and AP-1 activation. Both compounds significantly inhibited NF-kappaB activation by UVB, but AP-1 activation was unaffected by curcumin while PDTC further stimulated its activation. In parallel, curcumin decreased, in a dose-dependent manner, the UVB-mediated overexpression of all three pro-inflammatory cytokines and only exhibited a moderate enhancing influence on IL-10 expression. In turn, the inhibitory influence of PDTC on radiation-induced TNF-alpha and IL-6 expression is much lower and in contrast to curcumin, it stimulated IL-8. Taken together, our data indicated that control of proinflammatory cytokine expression induced by UVB in keratinocytes required the selective inhibition of NF-kappaB activation. Simultaneous AP-1 activation by agents like PDTC might, partially or totally, depending on cytokine-type, counterbalanced the inhibitory effect exerted on UVB-induced NF-kappaB activation in keratinocytes.

Authors+Show Affiliations

Laboratoire d'Immunologie (EA2070), IFR 53 Biomolécules, INSERM, CNRS, 1 avenue du Maréchal Juin, 51100, Reims, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12126654

Citation

Grandjean-Laquerriere, Alexia, et al. "Relative Contribution of NF-kappaB and AP-1 in the Modulation By Curcumin and Pyrrolidine Dithiocarbamate of the UVB-induced Cytokine Expression By Keratinocytes." Cytokine, vol. 18, no. 3, 2002, pp. 168-77.
Grandjean-Laquerriere A, Gangloff SC, Le Naour R, et al. Relative contribution of NF-kappaB and AP-1 in the modulation by curcumin and pyrrolidine dithiocarbamate of the UVB-induced cytokine expression by keratinocytes. Cytokine. 2002;18(3):168-77.
Grandjean-Laquerriere, A., Gangloff, S. C., Le Naour, R., Trentesaux, C., Hornebeck, W., & Guenounou, M. (2002). Relative contribution of NF-kappaB and AP-1 in the modulation by curcumin and pyrrolidine dithiocarbamate of the UVB-induced cytokine expression by keratinocytes. Cytokine, 18(3), 168-77.
Grandjean-Laquerriere A, et al. Relative Contribution of NF-kappaB and AP-1 in the Modulation By Curcumin and Pyrrolidine Dithiocarbamate of the UVB-induced Cytokine Expression By Keratinocytes. Cytokine. 2002 May 7;18(3):168-77. PubMed PMID: 12126654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative contribution of NF-kappaB and AP-1 in the modulation by curcumin and pyrrolidine dithiocarbamate of the UVB-induced cytokine expression by keratinocytes. AU - Grandjean-Laquerriere,Alexia, AU - Gangloff,Sophie C, AU - Le Naour,Richard, AU - Trentesaux,Chantal, AU - Hornebeck,William, AU - Guenounou,Moncef, PY - 2002/7/20/pubmed PY - 2003/3/4/medline PY - 2002/7/20/entrez SP - 168 EP - 77 JF - Cytokine JO - Cytokine VL - 18 IS - 3 N2 - Following ultraviolet B treatment, expression of tumour necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 by NCTC 2544 keratinocyte cell line was significantly enhanced both at the mRNA and protein level. The UVB also increased the IL-10 steady-state mRNAs level. Radiation-induced cytokine overexpression was accompanied by NF-kappaB and AP-1 transcription factors activation as assessed by electrophoretic mobility shift assays. To investigate in keratinocytes the relative contributions of those transcription factors on UVB-mediated cytokine induction, cell cultures were supplemented with curcumin and pyrrolidine dithiocarbamate (PDTC), agents known to modulate NF-kappaB and AP-1 activation. Both compounds significantly inhibited NF-kappaB activation by UVB, but AP-1 activation was unaffected by curcumin while PDTC further stimulated its activation. In parallel, curcumin decreased, in a dose-dependent manner, the UVB-mediated overexpression of all three pro-inflammatory cytokines and only exhibited a moderate enhancing influence on IL-10 expression. In turn, the inhibitory influence of PDTC on radiation-induced TNF-alpha and IL-6 expression is much lower and in contrast to curcumin, it stimulated IL-8. Taken together, our data indicated that control of proinflammatory cytokine expression induced by UVB in keratinocytes required the selective inhibition of NF-kappaB activation. Simultaneous AP-1 activation by agents like PDTC might, partially or totally, depending on cytokine-type, counterbalanced the inhibitory effect exerted on UVB-induced NF-kappaB activation in keratinocytes. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/12126654/Relative_contribution_of_NF_kappaB_and_AP_1_in_the_modulation_by_curcumin_and_pyrrolidine_dithiocarbamate_of_the_UVB_induced_cytokine_expression_by_keratinocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043466602908884 DB - PRIME DP - Unbound Medicine ER -