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Xenotransplantation of pig islets in diabetic dogs with use of a microcapsule composed of agarose and polystyrene sulfonic acid mixed gel.
Pancreas. 2002 Jul; 25(1):94-100.P

Abstract

INTRODUCTION

The authors have designed a microcapsule composed of agarose and polystyrene sulfonic acid (PSSa) mixed gel that provides a protective barrier against complement attack. Xenografts of islets, encapsulated in an agarose-PSSa microcapsule, have been shown to normalize blood glucose in rodents with chemically induced diabetes for extended periods of time without immunosuppression.

AIM

To investigate the efficacy of agarose-PSSa microencapsulated pig islets in reversing diabetes in a large animal model.

METHODOLOGY

Diabetes was induced in beagle recipients by total pancreatectomy. Each recipient received three to five intraperitoneal injections of either encapsulated (n = 5) or nonencapsulated pig islets (n = 2).

RESULTS

In all dogs receiving microencapsulated islets, the graft function was achieved for 7.4 +/- 3.1 weeks (mean +/- standard error), as determined by elimination or reduction of exogenous insulin requirement. In three recipients, the fasting blood glucose levels were maintained at < or = 200 mg/dL without any exogenous insulin for a period of 6, 50, and 119 days. Circulating porcine C-peptide was detected in the sera of all dogs after transplantation of encapsulated islets. Immunohistologic examination revealed the presence of insulin-positive cells in the microcapsules. In contrast, in two dogs receiving nonencapsulated islets there was no graft function.

CONCLUSIONS

This preliminary study demonstrates that agarose-PSSa microencapsulated pig islets can survive and function for weeks or months in totally pancreatectomized diabetic dogs without immunosuppression.

Authors+Show Affiliations

First Department of Surgery, Nara Medical University, Nara, Japan. tkin@gpu.srv.ualberta.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12131778

Citation

Kin, Tatsuya, et al. "Xenotransplantation of Pig Islets in Diabetic Dogs With Use of a Microcapsule Composed of Agarose and Polystyrene Sulfonic Acid Mixed Gel." Pancreas, vol. 25, no. 1, 2002, pp. 94-100.
Kin T, Iwata H, Aomatsu Y, et al. Xenotransplantation of pig islets in diabetic dogs with use of a microcapsule composed of agarose and polystyrene sulfonic acid mixed gel. Pancreas. 2002;25(1):94-100.
Kin, T., Iwata, H., Aomatsu, Y., Ohyama, T., Kanehiro, H., Hisanaga, M., & Nakajima, Y. (2002). Xenotransplantation of pig islets in diabetic dogs with use of a microcapsule composed of agarose and polystyrene sulfonic acid mixed gel. Pancreas, 25(1), 94-100.
Kin T, et al. Xenotransplantation of Pig Islets in Diabetic Dogs With Use of a Microcapsule Composed of Agarose and Polystyrene Sulfonic Acid Mixed Gel. Pancreas. 2002;25(1):94-100. PubMed PMID: 12131778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xenotransplantation of pig islets in diabetic dogs with use of a microcapsule composed of agarose and polystyrene sulfonic acid mixed gel. AU - Kin,Tatsuya, AU - Iwata,Hiroo, AU - Aomatsu,Yukio, AU - Ohyama,Takao, AU - Kanehiro,Hiromichi, AU - Hisanaga,Michiyoshi, AU - Nakajima,Yoshiyuki, PY - 2002/7/20/pubmed PY - 2003/1/11/medline PY - 2002/7/20/entrez SP - 94 EP - 100 JF - Pancreas JO - Pancreas VL - 25 IS - 1 N2 - INTRODUCTION: The authors have designed a microcapsule composed of agarose and polystyrene sulfonic acid (PSSa) mixed gel that provides a protective barrier against complement attack. Xenografts of islets, encapsulated in an agarose-PSSa microcapsule, have been shown to normalize blood glucose in rodents with chemically induced diabetes for extended periods of time without immunosuppression. AIM: To investigate the efficacy of agarose-PSSa microencapsulated pig islets in reversing diabetes in a large animal model. METHODOLOGY: Diabetes was induced in beagle recipients by total pancreatectomy. Each recipient received three to five intraperitoneal injections of either encapsulated (n = 5) or nonencapsulated pig islets (n = 2). RESULTS: In all dogs receiving microencapsulated islets, the graft function was achieved for 7.4 +/- 3.1 weeks (mean +/- standard error), as determined by elimination or reduction of exogenous insulin requirement. In three recipients, the fasting blood glucose levels were maintained at < or = 200 mg/dL without any exogenous insulin for a period of 6, 50, and 119 days. Circulating porcine C-peptide was detected in the sera of all dogs after transplantation of encapsulated islets. Immunohistologic examination revealed the presence of insulin-positive cells in the microcapsules. In contrast, in two dogs receiving nonencapsulated islets there was no graft function. CONCLUSIONS: This preliminary study demonstrates that agarose-PSSa microencapsulated pig islets can survive and function for weeks or months in totally pancreatectomized diabetic dogs without immunosuppression. SN - 0885-3177 UR - https://www.unboundmedicine.com/medline/citation/12131778/Xenotransplantation_of_pig_islets_in_diabetic_dogs_with_use_of_a_microcapsule_composed_of_agarose_and_polystyrene_sulfonic_acid_mixed_gel_ L2 - https://doi.org/10.1097/00006676-200207000-00020 DB - PRIME DP - Unbound Medicine ER -