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[Losartan in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of monocyte chemoattractant protein-1 and basic fibroblast growth factor].
Zhonghua Jie He He Hu Xi Za Zhi. 2002 May; 25(5):268-72.ZJ

Abstract

OBJECTIVE

To study the effect of losartan on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.

METHODS

Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin. Then the rats received daily losartan 10 mg/kg (group L), prednisone 20 mg/kg (group P), or normal saline (group B) orally. Normal controls received normal saline both intratracheally and orally. Six rats in each group were sacrificed 3, 7, 14, 28 and 56 days after intratracheal instillation. Histological changes of the lungs were evaluated by HE stain, Masson's trichrome stain and sirius red stain. Collagen content of the lung tissue was assessed by hydroxyproline concentration. The mRNAs of monocyte chemoattractant protein-1 (MCP-1) and basic fibroblast growth factor (bFGF) were detected by reverse transcription-polymerase chain reaction (RT-PCR). Lung expression of MCP-1 and bFGF proteins was assessed by immunohistochemistry and the level of MCP-1 protein was further measured by ELISA.

RESULTS

Pulmonary fibrosis of group L and group P was improved as compared with that of group B. Hydroxyproline concentrations of group L and group P were lower than that of group B. Protein expression of MCP-1 and bFGF in group L was lower than that in group B.

CONCLUSION

Losartan alleviates bleomycin-induced pulmonary fibrosis in rats. Inhibiting the expressions of MCP-1 and bFGF in lung tissues may be one of the mechanisms.

Authors+Show Affiliations

Department of Respiratory Disease, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12133317

Citation

Fang, Xiangqun, et al. "[Losartan in the Rat Model of Bleomycin-induced Pulmonary Fibrosis and Its Impact On the Expression of Monocyte Chemoattractant Protein-1 and Basic Fibroblast Growth Factor]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 25, no. 5, 2002, pp. 268-72.
Fang X, Zhu Y, Hu X, et al. [Losartan in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of monocyte chemoattractant protein-1 and basic fibroblast growth factor]. Zhonghua Jie He He Hu Xi Za Zhi. 2002;25(5):268-72.
Fang, X., Zhu, Y., Hu, X., & Liu, Y. (2002). [Losartan in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of monocyte chemoattractant protein-1 and basic fibroblast growth factor]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 25(5), 268-72.
Fang X, et al. [Losartan in the Rat Model of Bleomycin-induced Pulmonary Fibrosis and Its Impact On the Expression of Monocyte Chemoattractant Protein-1 and Basic Fibroblast Growth Factor]. Zhonghua Jie He He Hu Xi Za Zhi. 2002;25(5):268-72. PubMed PMID: 12133317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Losartan in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of monocyte chemoattractant protein-1 and basic fibroblast growth factor]. AU - Fang,Xiangqun, AU - Zhu,Yuanjue, AU - Hu,Xiaoling, AU - Liu,Yongjian, PY - 2002/7/23/pubmed PY - 2002/9/14/medline PY - 2002/7/23/entrez SP - 268 EP - 72 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 25 IS - 5 N2 - OBJECTIVE: To study the effect of losartan on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism. METHODS: Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin. Then the rats received daily losartan 10 mg/kg (group L), prednisone 20 mg/kg (group P), or normal saline (group B) orally. Normal controls received normal saline both intratracheally and orally. Six rats in each group were sacrificed 3, 7, 14, 28 and 56 days after intratracheal instillation. Histological changes of the lungs were evaluated by HE stain, Masson's trichrome stain and sirius red stain. Collagen content of the lung tissue was assessed by hydroxyproline concentration. The mRNAs of monocyte chemoattractant protein-1 (MCP-1) and basic fibroblast growth factor (bFGF) were detected by reverse transcription-polymerase chain reaction (RT-PCR). Lung expression of MCP-1 and bFGF proteins was assessed by immunohistochemistry and the level of MCP-1 protein was further measured by ELISA. RESULTS: Pulmonary fibrosis of group L and group P was improved as compared with that of group B. Hydroxyproline concentrations of group L and group P were lower than that of group B. Protein expression of MCP-1 and bFGF in group L was lower than that in group B. CONCLUSION: Losartan alleviates bleomycin-induced pulmonary fibrosis in rats. Inhibiting the expressions of MCP-1 and bFGF in lung tissues may be one of the mechanisms. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/12133317/[Losartan_in_the_rat_model_of_bleomycin_induced_pulmonary_fibrosis_and_its_impact_on_the_expression_of_monocyte_chemoattractant_protein_1_and_basic_fibroblast_growth_factor]_ L2 - https://medlineplus.gov/pulmonaryfibrosis.html DB - PRIME DP - Unbound Medicine ER -