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[Nitric oxide modulates expression of matrix metalloproteinase in asthmatic rats].
Zhonghua Jie He He Hu Xi Za Zhi. 2002 May; 25(5):276-9.ZJ

Abstract

OBJECTIVE

To observe the effect of nitric oxide on expression of matrix metalloproteinase and tissue inhibitor of metalloproteinase in asthmatic rats.

METHODS

Thirty healthy male Wistar rats were randomly divided into control (n = 10), asthmatic (n = 10) and L-arginine (n = 10) groups. Lung tissues were sliced and stained with HE. The following morphometric parameters were measured by image analysis system: airway wall basement membrane perimeter (Pbm), total bronchial wall area (WAt), the inner wall area (WAi) and the area of smooth muscle (WAm). Levels of nitrites/nitrates and NOS activity in the lungs were measured by commercial NO and NOS kits. Transcriptional levels of MMP-2 and TIMP-1 mRNA in the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS

(1) WAt/Pbm, WAi/Pbm and WAm/Pbm in the L-arginine group were (35.1 +/- 2.6) microm(2)/microm, (25.3 +/- 2.0) microm(2)/microm, and (8.7 +/- 1.5) microm(2)/microm, respectively. WAt/Pbm, WAi/Pbm and WAm/Pbm in the asthmatic group were (25.3 +/- 2.1) microm(2)/microm, (20.4 +/- 2.3) microm(2)/microm, and (4.2 +/- 2.0) microm(2)/microm, respectively. WAt/Pbm, WAi/Pbm and WAm/Pbm in the control group were (20.8 +/- 1.3) microm(2)/microm, (15.3 +/- 2.1) microm(2)/microm, and (3.1 +/- 1.1) microm(2)/microm, respectively. The differences among three groups were statistically significant. (2) Levels of nitrites/nitrates in the L-arginine group [(11.8 +/- 1.7) nmol/mg] and the asthmatic group [(7.2 +/- 2.1) nmol/mg] were significantly higher than that in the control group [(3.1 +/- 1.2) nmol/mg]. NOS activity in the lungs was (16.5 +/- 1.3) U/mg L-arginine group, (10.8 +/- 1.4) U/mg asthmatic group, and (1.46 +/- 0.98) U/mg control group, respectively, the differences being statistically significant between groups. (3) MMP-2 and TIMP-1 mRNA levels in the L-arginine group and the asthmatic group were significantly higher than that in the control group [L-arginine group (0.82 +/- 0.11), (0.51 +/- 0.12); asthmatic group (0.68 +/- 0.14), (0.56 +/- 0.10); control group (0.14 +/- 0.03), (0.11 +/- 0.05), respectively]. The difference in the MMP-2 mRNA levels was significant between the L-arginine group and the asthmatic group (P < 0.05), but the difference in the TIMP-1 mRNA levels between the two groups was not statistically significant. (P > 0.05). (4) A significant positive correlation was found between the MMP-2 mRNA levels and nitrites/nitrates levels in L-arginine group and asthmatic group (r(s) = 0.65, 0.68, P < 0.05), but there was no significant correlation between the nitrites/nitrates levels and the TIMP-1 mRNA levels in the two groups (r(s) = 0.23, 0.18, P > 0.05).

CONCLUSION

Nitric oxide may contribute to airway inflammation and remodeling by influencing the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinases.

Authors+Show Affiliations

Department of Geriatrics, The Huaxi Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12133319

Citation

Yuan, Yiming, et al. "[Nitric Oxide Modulates Expression of Matrix Metalloproteinase in Asthmatic Rats]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 25, no. 5, 2002, pp. 276-9.
Yuan Y, Wang Z, Dong B. [Nitric oxide modulates expression of matrix metalloproteinase in asthmatic rats]. Zhonghua Jie He He Hu Xi Za Zhi. 2002;25(5):276-9.
Yuan, Y., Wang, Z., & Dong, B. (2002). [Nitric oxide modulates expression of matrix metalloproteinase in asthmatic rats]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 25(5), 276-9.
Yuan Y, Wang Z, Dong B. [Nitric Oxide Modulates Expression of Matrix Metalloproteinase in Asthmatic Rats]. Zhonghua Jie He He Hu Xi Za Zhi. 2002;25(5):276-9. PubMed PMID: 12133319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Nitric oxide modulates expression of matrix metalloproteinase in asthmatic rats]. AU - Yuan,Yiming, AU - Wang,Zengli, AU - Dong,Birong, PY - 2002/7/23/pubmed PY - 2002/9/14/medline PY - 2002/7/23/entrez SP - 276 EP - 9 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 25 IS - 5 N2 - OBJECTIVE: To observe the effect of nitric oxide on expression of matrix metalloproteinase and tissue inhibitor of metalloproteinase in asthmatic rats. METHODS: Thirty healthy male Wistar rats were randomly divided into control (n = 10), asthmatic (n = 10) and L-arginine (n = 10) groups. Lung tissues were sliced and stained with HE. The following morphometric parameters were measured by image analysis system: airway wall basement membrane perimeter (Pbm), total bronchial wall area (WAt), the inner wall area (WAi) and the area of smooth muscle (WAm). Levels of nitrites/nitrates and NOS activity in the lungs were measured by commercial NO and NOS kits. Transcriptional levels of MMP-2 and TIMP-1 mRNA in the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: (1) WAt/Pbm, WAi/Pbm and WAm/Pbm in the L-arginine group were (35.1 +/- 2.6) microm(2)/microm, (25.3 +/- 2.0) microm(2)/microm, and (8.7 +/- 1.5) microm(2)/microm, respectively. WAt/Pbm, WAi/Pbm and WAm/Pbm in the asthmatic group were (25.3 +/- 2.1) microm(2)/microm, (20.4 +/- 2.3) microm(2)/microm, and (4.2 +/- 2.0) microm(2)/microm, respectively. WAt/Pbm, WAi/Pbm and WAm/Pbm in the control group were (20.8 +/- 1.3) microm(2)/microm, (15.3 +/- 2.1) microm(2)/microm, and (3.1 +/- 1.1) microm(2)/microm, respectively. The differences among three groups were statistically significant. (2) Levels of nitrites/nitrates in the L-arginine group [(11.8 +/- 1.7) nmol/mg] and the asthmatic group [(7.2 +/- 2.1) nmol/mg] were significantly higher than that in the control group [(3.1 +/- 1.2) nmol/mg]. NOS activity in the lungs was (16.5 +/- 1.3) U/mg L-arginine group, (10.8 +/- 1.4) U/mg asthmatic group, and (1.46 +/- 0.98) U/mg control group, respectively, the differences being statistically significant between groups. (3) MMP-2 and TIMP-1 mRNA levels in the L-arginine group and the asthmatic group were significantly higher than that in the control group [L-arginine group (0.82 +/- 0.11), (0.51 +/- 0.12); asthmatic group (0.68 +/- 0.14), (0.56 +/- 0.10); control group (0.14 +/- 0.03), (0.11 +/- 0.05), respectively]. The difference in the MMP-2 mRNA levels was significant between the L-arginine group and the asthmatic group (P < 0.05), but the difference in the TIMP-1 mRNA levels between the two groups was not statistically significant. (P > 0.05). (4) A significant positive correlation was found between the MMP-2 mRNA levels and nitrites/nitrates levels in L-arginine group and asthmatic group (r(s) = 0.65, 0.68, P < 0.05), but there was no significant correlation between the nitrites/nitrates levels and the TIMP-1 mRNA levels in the two groups (r(s) = 0.23, 0.18, P > 0.05). CONCLUSION: Nitric oxide may contribute to airway inflammation and remodeling by influencing the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinases. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/12133319/[Nitric_oxide_modulates_expression_of_matrix_metalloproteinase_in_asthmatic_rats]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -