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[Screening substances derived from cultures of medicinal plants for antimutagenic activity in the Escherichia coli-bacteriophage lambda system].
Tsitol Genet. 2002 Mar-Apr; 36(2):3-10.TG

Abstract

In Escherichia coli--bacteriophage lambda system protective properties of the extracts derived from the biomass of cultured Panax ginseng, Polyscias filicifolia, Rhodiola rosea, Ungernia victoris cells, and those from intact Rhodiola roots have been studied. Escherichia coli--bacteriophage lambda system responsiveness was found to vary with the test-object state, namely: the deleted bacteriophage form (lambda-4) as well as previously mutagenized bacteriophage were more sensitive to the mutagenic and antimutagenic influence versus the native bacteriophage lambda +. The contribution of extracts in the induction and realization of the lethal injuries in phages caused by nitrite acid in extracellular phage (conditions in vitro) was estimated thus enabling to discriminate between the protective and antimutagenic extract activities. Protective extract effect in the given test-system appeared to be higher their antimutagenic action. With the most responsive bacteriophage variant the extracts from the biomass of cultured Rh. rosea and P. filicifolia cells showed high protective and somewhat lower antimutagenic activities. With other phages significant antimutagenic potential of extracts was demonstrated, which by their protective effect could be arranged in a raw as follows U. victoris > P. ginseng > P. filicifolia. The primary screening for the antimutagenic effect of preparations in the prokaryotic systems could be reduced to the investigation of their effects on the object inactivation exposed to the mutagen in vitro.

Authors

Dvornyk AS
No affiliation info available
Pererva TP
No affiliation info available
Kukakh VA
No affiliation info available

MeSH

Antimutagenic AgentsBacteriophage lambdaDrug Evaluation, PreclinicalEscherichia coliPlant ExtractsPlants, Medicinal

Pub Type(s)

Journal Article

Language

ukr

PubMed ID

12140895

Citation

Dvornyk, A S., et al. "[Screening Substances Derived From Cultures of Medicinal Plants for Antimutagenic Activity in the Escherichia Coli-bacteriophage Lambda System]." TSitologiia I Genetika, vol. 36, no. 2, 2002, pp. 3-10.
Dvornyk AS, Pererva TP, Kukakh VA. [Screening substances derived from cultures of medicinal plants for antimutagenic activity in the Escherichia coli-bacteriophage lambda system]. Tsitol Genet. 2002;36(2):3-10.
Dvornyk, A. S., Pererva, T. P., & Kukakh, V. A. (2002). [Screening substances derived from cultures of medicinal plants for antimutagenic activity in the Escherichia coli-bacteriophage lambda system]. TSitologiia I Genetika, 36(2), 3-10.
Dvornyk AS, Pererva TP, Kukakh VA. [Screening Substances Derived From Cultures of Medicinal Plants for Antimutagenic Activity in the Escherichia Coli-bacteriophage Lambda System]. Tsitol Genet. 2002 Mar-Apr;36(2):3-10. PubMed PMID: 12140895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Screening substances derived from cultures of medicinal plants for antimutagenic activity in the Escherichia coli-bacteriophage lambda system]. AU - Dvornyk,A S, AU - Pererva,T P, AU - Kukakh,V A, PY - 2002/7/27/pubmed PY - 2002/9/26/medline PY - 2002/7/27/entrez SP - 3 EP - 10 JF - TSitologiia i genetika JO - Tsitol Genet VL - 36 IS - 2 N2 - In Escherichia coli--bacteriophage lambda system protective properties of the extracts derived from the biomass of cultured Panax ginseng, Polyscias filicifolia, Rhodiola rosea, Ungernia victoris cells, and those from intact Rhodiola roots have been studied. Escherichia coli--bacteriophage lambda system responsiveness was found to vary with the test-object state, namely: the deleted bacteriophage form (lambda-4) as well as previously mutagenized bacteriophage were more sensitive to the mutagenic and antimutagenic influence versus the native bacteriophage lambda +. The contribution of extracts in the induction and realization of the lethal injuries in phages caused by nitrite acid in extracellular phage (conditions in vitro) was estimated thus enabling to discriminate between the protective and antimutagenic extract activities. Protective extract effect in the given test-system appeared to be higher their antimutagenic action. With the most responsive bacteriophage variant the extracts from the biomass of cultured Rh. rosea and P. filicifolia cells showed high protective and somewhat lower antimutagenic activities. With other phages significant antimutagenic potential of extracts was demonstrated, which by their protective effect could be arranged in a raw as follows U. victoris > P. ginseng > P. filicifolia. The primary screening for the antimutagenic effect of preparations in the prokaryotic systems could be reduced to the investigation of their effects on the object inactivation exposed to the mutagen in vitro. SN - 0564-3783 UR - https://www.unboundmedicine.com/medline/citation/12140895/[Screening_substances_derived_from_cultures_of_medicinal_plants_for_antimutagenic_activity_in_the_Escherichia_coli_bacteriophage_lambda_system]_ DB - PRIME DP - Unbound Medicine ER -