Tags

Type your tag names separated by a space and hit enter

Strategies for diagnosis of HTLV-I and -II.
Transfusion. 2002 Jun; 42(6):780-91.T

Abstract

BACKGROUND

No gold standard exists for diagnosis of HTLV infection. The aim of thus study was to compare the accuracy of a combination of two sensitive ELISAs with Western blot (WB), a line immunoassay, and PCR for diagnosis of HTLV infection.

STUDY DESIGN AND METHODS

Nine hundred eighty-five specimens were tested for the presence of HTLV antibodies by HTLV-I and/or HTLV-II EIAs (Murex and Ortho), WB (Diagnostic Biotechnology), line immunoassay (INNO-LIA, Innogenetics), and/or presence of HTLV DNA by PCR. The results were compared with the probable HTLV infection status of each subject, as determined by detailed review of all available laboratory, clinical, and epidemiologic data.

RESULTS

The sensitivity for diagnosis of HTLV-I infection was high for all assays evaluated, but both PCR and WB had a lower sensitivity rate (approx., 80%) for confirmation of HTLV-II. INNO-LIA detected 94 percent of the HTLV-II-positive samples. However, Murex EIA in combination with Ortho EIA was 100-percent sensitive for the detection of both HTLV-I and HTLV-II antibodies. Furthermore, the number of samples giving indeterminate results in the ELISA combination was much lower as compared with WB (2.5% vs. 50%).

CONCLUSION

Based on these findings, a new, more sensitive and specific test strategy for HTLV diagnosis than the current algorithm, which includes WB, is proposed. Thereby, both the direct and indirect costs can be substantially reduced.

Authors+Show Affiliations

Department of Immunology, Swedish Institute for Infectious Disease Control, Solna, Sweden. rigmor.thorstensson@smi.ki.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

12147033

Citation

Thorstensson, Rigmor, et al. "Strategies for Diagnosis of HTLV-I and -II." Transfusion, vol. 42, no. 6, 2002, pp. 780-91.
Thorstensson R, Albert J, Andersson S. Strategies for diagnosis of HTLV-I and -II. Transfusion. 2002;42(6):780-91.
Thorstensson, R., Albert, J., & Andersson, S. (2002). Strategies for diagnosis of HTLV-I and -II. Transfusion, 42(6), 780-91.
Thorstensson R, Albert J, Andersson S. Strategies for Diagnosis of HTLV-I and -II. Transfusion. 2002;42(6):780-91. PubMed PMID: 12147033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strategies for diagnosis of HTLV-I and -II. AU - Thorstensson,Rigmor, AU - Albert,Jon, AU - Andersson,Sören, PY - 2002/7/31/pubmed PY - 2002/8/24/medline PY - 2002/7/31/entrez SP - 780 EP - 91 JF - Transfusion JO - Transfusion VL - 42 IS - 6 N2 - BACKGROUND: No gold standard exists for diagnosis of HTLV infection. The aim of thus study was to compare the accuracy of a combination of two sensitive ELISAs with Western blot (WB), a line immunoassay, and PCR for diagnosis of HTLV infection. STUDY DESIGN AND METHODS: Nine hundred eighty-five specimens were tested for the presence of HTLV antibodies by HTLV-I and/or HTLV-II EIAs (Murex and Ortho), WB (Diagnostic Biotechnology), line immunoassay (INNO-LIA, Innogenetics), and/or presence of HTLV DNA by PCR. The results were compared with the probable HTLV infection status of each subject, as determined by detailed review of all available laboratory, clinical, and epidemiologic data. RESULTS: The sensitivity for diagnosis of HTLV-I infection was high for all assays evaluated, but both PCR and WB had a lower sensitivity rate (approx., 80%) for confirmation of HTLV-II. INNO-LIA detected 94 percent of the HTLV-II-positive samples. However, Murex EIA in combination with Ortho EIA was 100-percent sensitive for the detection of both HTLV-I and HTLV-II antibodies. Furthermore, the number of samples giving indeterminate results in the ELISA combination was much lower as compared with WB (2.5% vs. 50%). CONCLUSION: Based on these findings, a new, more sensitive and specific test strategy for HTLV diagnosis than the current algorithm, which includes WB, is proposed. Thereby, both the direct and indirect costs can be substantially reduced. SN - 0041-1132 UR - https://www.unboundmedicine.com/medline/citation/12147033/Strategies_for_diagnosis_of_HTLV_I_and__II_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0041-1132&date=2002&volume=42&issue=6&spage=780 DB - PRIME DP - Unbound Medicine ER -