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Partial nucleotide sequencing of hepatitis E viruses detected in sera of patients with hepatitis E from 14 cities in China.
Chin Med J (Engl). 2002 Jul; 115(7):1058-63.CM

Abstract

OBJECTIVE

To investigate the genotypes of hepatitis E viruses (HEV) detected in sera of patients from different regions of China.

METHODS

The partial genome (nt6461-6860, nt5994-6294) of open reading frame 2 (ORF2) of 45 HEV strains detected from 14 cities of China was amplified and sequenced using polymerase chain reaction (PCR) and direct sequencing.

RESULTS

Forty-one of 45 strains (91%) share the same genotype with HEV Burma strain (B), with nucleotide identities higher than 98% with the representative HEV Chinese strain. Only 4 HEV strains are significantly divergent from the 3 prototype strains of HEV, with nucleotide identities of 77%-80% with HEV Burmese/Chinese strain, 74%-76% with Mexican strain and 74%-77% with the newly discovered HEV US/swine strain, respectively. Phylogenetic analysis suggests that these 4 strains may represent 2 different subtypes that belong to a novel genotype of HEV, which is significantly divergent from the prototype Mexico, Burmese and US/swine strains.

CONCLUSION

Among patients with hepatitis E in China, most are infected by the Chinese prototype HEV, and only a small part by the new genotype HEV.

Authors+Show Affiliations

Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12150743

Citation

Li, Kui, et al. "Partial Nucleotide Sequencing of Hepatitis E Viruses Detected in Sera of Patients With Hepatitis E From 14 Cities in China." Chinese Medical Journal, vol. 115, no. 7, 2002, pp. 1058-63.
Li K, Zhuang H, Zhu W. Partial nucleotide sequencing of hepatitis E viruses detected in sera of patients with hepatitis E from 14 cities in China. Chin Med J (Engl). 2002;115(7):1058-63.
Li, K., Zhuang, H., & Zhu, W. (2002). Partial nucleotide sequencing of hepatitis E viruses detected in sera of patients with hepatitis E from 14 cities in China. Chinese Medical Journal, 115(7), 1058-63.
Li K, Zhuang H, Zhu W. Partial Nucleotide Sequencing of Hepatitis E Viruses Detected in Sera of Patients With Hepatitis E From 14 Cities in China. Chin Med J (Engl). 2002;115(7):1058-63. PubMed PMID: 12150743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Partial nucleotide sequencing of hepatitis E viruses detected in sera of patients with hepatitis E from 14 cities in China. AU - Li,Kui, AU - Zhuang,Hui, AU - Zhu,Wanfu, PY - 2002/8/2/pubmed PY - 2002/9/11/medline PY - 2002/8/2/entrez SP - 1058 EP - 63 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 115 IS - 7 N2 - OBJECTIVE: To investigate the genotypes of hepatitis E viruses (HEV) detected in sera of patients from different regions of China. METHODS: The partial genome (nt6461-6860, nt5994-6294) of open reading frame 2 (ORF2) of 45 HEV strains detected from 14 cities of China was amplified and sequenced using polymerase chain reaction (PCR) and direct sequencing. RESULTS: Forty-one of 45 strains (91%) share the same genotype with HEV Burma strain (B), with nucleotide identities higher than 98% with the representative HEV Chinese strain. Only 4 HEV strains are significantly divergent from the 3 prototype strains of HEV, with nucleotide identities of 77%-80% with HEV Burmese/Chinese strain, 74%-76% with Mexican strain and 74%-77% with the newly discovered HEV US/swine strain, respectively. Phylogenetic analysis suggests that these 4 strains may represent 2 different subtypes that belong to a novel genotype of HEV, which is significantly divergent from the prototype Mexico, Burmese and US/swine strains. CONCLUSION: Among patients with hepatitis E in China, most are infected by the Chinese prototype HEV, and only a small part by the new genotype HEV. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/12150743/Partial_nucleotide_sequencing_of_hepatitis_E_viruses_detected_in_sera_of_patients_with_hepatitis_E_from_14_cities_in_China_ L2 - https://www.diseaseinfosearch.org/result/3334 DB - PRIME DP - Unbound Medicine ER -