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Development of a set of Triticum aestivum-Aegilops tauschii introgression lines.
Hereditas. 2001; 135(2-3):139-43.H

Abstract

New wheat introgression lines were obtained which contain different segments of individual chromosomes of Aegilops tauschii in the Triticum aestivum cv. 'Chinese Spring' background. The introgression lines were developed to examine various subsets of alleles from the wild grass in the genetic background of common wheat. As starting point substitution lines of 'Chinese Spring' in which single chromosomes of the D genome had been replaced by homologous chromosomes of a synthetic wheat were used. Synthetic wheat had been obtained earlier from a cross between the tetraploid emmer (genomes AABB) and wild grass Aegilops tauschii (genome DD). The seven wheat chromosome substitution lines carrying different chromosomes of Ae. tauschii were crossed twice to T. aestivum cv. 'Chinese Spring' and 259 BC1-progeny plants were analysed. Phenotypic evaluation was carried out for different traits such as plant height, spikelet number, peduncle length, flowering time, spike length, tiller number, grain weight per ear, fertility and thousand kernel weight. Genotypic analysis was performed using a set of 65 microsatellite markers previously mapped on the chromosomes of the D genome of wheat. During this analysis recombinant lines carrying different segments of Ae. tauschii chromosomes were detected. Plants containing small introgressions of the alien genetic material were selfed to get homozygous lines and plants carrying large pieces of the donor chromosome were backcrossed again to get smaller introgressions. Further microsatellite analysis of selected BC1F2-progeny plants resulted in detection of a first set of 36 homozygous lines carrying different pieces of Ae. tauschii genome.

Authors+Show Affiliations

Pestsova EG
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany. pestsova@ipk-gatersleben.de
Börner A
No affiliation info available
Röder MS
No affiliation info available

MeSH

AllelesChromosomesCrosses, GeneticGenome, PlantHomozygoteMicrosatellite RepeatsModels, GeneticPhenotypeQuantitative Trait, HeritableTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12152326

Citation

Pestsova, E G., et al. "Development of a Set of Triticum aestivum-Aegilops Tauschii Introgression Lines." Hereditas, vol. 135, no. 2-3, 2001, pp. 139-43.
Pestsova EG, Börner A, Röder MS. Development of a set of Triticum aestivum-Aegilops tauschii introgression lines. Hereditas. 2001;135(2-3):139-43.
Pestsova, E. G., Börner, A., & Röder, M. S. (2001). Development of a set of Triticum aestivum-Aegilops tauschii introgression lines. Hereditas, 135(2-3), 139-43.
Pestsova EG, Börner A, Röder MS. Development of a Set of Triticum aestivum-Aegilops Tauschii Introgression Lines. Hereditas. 2001;135(2-3):139-43. PubMed PMID: 12152326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a set of Triticum aestivum-Aegilops tauschii introgression lines. AU - Pestsova,E G, AU - Börner,A, AU - Röder,M S, PY - 2002/8/3/pubmed PY - 2002/8/22/medline PY - 2002/8/3/entrez SP - 139 EP - 43 JF - Hereditas JO - Hereditas VL - 135 IS - 2-3 N2 - New wheat introgression lines were obtained which contain different segments of individual chromosomes of Aegilops tauschii in the Triticum aestivum cv. 'Chinese Spring' background. The introgression lines were developed to examine various subsets of alleles from the wild grass in the genetic background of common wheat. As starting point substitution lines of 'Chinese Spring' in which single chromosomes of the D genome had been replaced by homologous chromosomes of a synthetic wheat were used. Synthetic wheat had been obtained earlier from a cross between the tetraploid emmer (genomes AABB) and wild grass Aegilops tauschii (genome DD). The seven wheat chromosome substitution lines carrying different chromosomes of Ae. tauschii were crossed twice to T. aestivum cv. 'Chinese Spring' and 259 BC1-progeny plants were analysed. Phenotypic evaluation was carried out for different traits such as plant height, spikelet number, peduncle length, flowering time, spike length, tiller number, grain weight per ear, fertility and thousand kernel weight. Genotypic analysis was performed using a set of 65 microsatellite markers previously mapped on the chromosomes of the D genome of wheat. During this analysis recombinant lines carrying different segments of Ae. tauschii chromosomes were detected. Plants containing small introgressions of the alien genetic material were selfed to get homozygous lines and plants carrying large pieces of the donor chromosome were backcrossed again to get smaller introgressions. Further microsatellite analysis of selected BC1F2-progeny plants resulted in detection of a first set of 36 homozygous lines carrying different pieces of Ae. tauschii genome. SN - 0018-0661 UR - https://www.unboundmedicine.com/medline/citation/12152326/Development_of_a_set_of_Triticum_aestivum_Aegilops_tauschii_introgression_lines_ DB - PRIME DP - Unbound Medicine ER -