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Use of the highly sensitive PCR method to detect the Herpes simplex virus type 1 genome and its expression in samples from Behçet disease patients.
J Med Dent Sci. 1998 Mar; 45(1):51-8.JM

Abstract

To study the involvement of the Herpes simplex virus type 1 (HSV-1) in patients with Behçet's disease (BD), PCR was used to detect HSV-1 DNA and RNA (LAT, TK, gB) in peripheral blood leukocytes and oral smears from patients with BD. Out of 32 patients with BD and 30 healthy volunteers, HSV-1 specific DNA was not detected in any case. Using a highly sensitive competitive RT-PCR method, HSV-1 specific mRNA was not detected either in peripheral blood leukocytes from any of the patients or volunteers. In contrast, serum levels of IgG anti HSV-1 antibody were higher in patients with BD; however, there were 70% sero-positives among the control subjects. Even though it has been reported that the highly frequent presence of the HSV-1 genome in peripheral blood is a characteristic feature of BD, our results show that the HSV-1 genome and its expression are not present in peripheral blood in patients with BD. We could not find any evidence that the lesions associated with BD were caused by the direct lytic effects of HSV-1.

Authors+Show Affiliations

Department of Preventive Medicine, Medical Research Institute, Tokyo Medical and Dental University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12160245

Citation

Nomura, Y, et al. "Use of the Highly Sensitive PCR Method to Detect the Herpes Simplex Virus Type 1 Genome and Its Expression in Samples From Behçet Disease Patients." Journal of Medical and Dental Sciences, vol. 45, no. 1, 1998, pp. 51-8.
Nomura Y, Kitteringham N, Shiba K, et al. Use of the highly sensitive PCR method to detect the Herpes simplex virus type 1 genome and its expression in samples from Behçet disease patients. J Med Dent Sci. 1998;45(1):51-8.
Nomura, Y., Kitteringham, N., Shiba, K., Goseki, M., Kimura, A., & Mineshita, S. (1998). Use of the highly sensitive PCR method to detect the Herpes simplex virus type 1 genome and its expression in samples from Behçet disease patients. Journal of Medical and Dental Sciences, 45(1), 51-8.
Nomura Y, et al. Use of the Highly Sensitive PCR Method to Detect the Herpes Simplex Virus Type 1 Genome and Its Expression in Samples From Behçet Disease Patients. J Med Dent Sci. 1998;45(1):51-8. PubMed PMID: 12160245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of the highly sensitive PCR method to detect the Herpes simplex virus type 1 genome and its expression in samples from Behçet disease patients. AU - Nomura,Y, AU - Kitteringham,N, AU - Shiba,K, AU - Goseki,M, AU - Kimura,A, AU - Mineshita,S, PY - 2002/8/6/pubmed PY - 2002/8/14/medline PY - 2002/8/6/entrez SP - 51 EP - 8 JF - Journal of medical and dental sciences JO - J Med Dent Sci VL - 45 IS - 1 N2 - To study the involvement of the Herpes simplex virus type 1 (HSV-1) in patients with Behçet's disease (BD), PCR was used to detect HSV-1 DNA and RNA (LAT, TK, gB) in peripheral blood leukocytes and oral smears from patients with BD. Out of 32 patients with BD and 30 healthy volunteers, HSV-1 specific DNA was not detected in any case. Using a highly sensitive competitive RT-PCR method, HSV-1 specific mRNA was not detected either in peripheral blood leukocytes from any of the patients or volunteers. In contrast, serum levels of IgG anti HSV-1 antibody were higher in patients with BD; however, there were 70% sero-positives among the control subjects. Even though it has been reported that the highly frequent presence of the HSV-1 genome in peripheral blood is a characteristic feature of BD, our results show that the HSV-1 genome and its expression are not present in peripheral blood in patients with BD. We could not find any evidence that the lesions associated with BD were caused by the direct lytic effects of HSV-1. SN - 1342-8810 UR - https://www.unboundmedicine.com/medline/citation/12160245/Use_of_the_highly_sensitive_PCR_method_to_detect_the_Herpes_simplex_virus_type_1_genome_and_its_expression_in_samples_from_Behçet_disease_patients_ L2 - http://www.diseaseinfosearch.org/result/780 DB - PRIME DP - Unbound Medicine ER -