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Rapid simultaneous determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in urine by solid-phase extraction and GC-MS: a method optimized for high-volume laboratories.
J Anal Toxicol. 2002 Jul-Aug; 26(5):253-61.JA

Abstract

To facilitate analysis of high sample volumes, an extraction, derivatization and gas chromatographic-mass spectrometric analysis method was developed to simultaneously determine amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA) 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) in urine. This method utilized a positive-pressure manifold cation-exchange polymer-based solid-phase extraction followed by elution directly into automated liquid sampler (ALS) vials. Rapid derivatization was accomplished using heptafluorobutyric anhydride (HFBA). Recoveries averaged 90% or greater for each of the compounds. Limits of detection were 62.5 ng/mL (AMP and MDEA), 15.6 ng/mL (MAMP), and 31.3 ng/mL (MDA and MDMA) using a 2-mL sample volume. The method was linear to 5000 ng/mL for all compounds using MDMA-d5 and MAMP-d14 as internal standards. Over 200 human urine samples previously determined to contain the target analytes were analyzed using the method. Excellent agreement was seen with previous quantitations. The method was challenged with 75 potentially interfering compounds and no interferences were seen. These interfering compounds included ephedrine, pseudoephedrine, phenylpropanolamine, and phenethylamine. The method resulted in dramatic reductions in processing time and waste production.

Authors+Show Affiliations

Navy Drug Screening Laboratory, Naval Air Station, Jacksonville, Florida 32212, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12166811

Citation

Stout, Peter R., et al. "Rapid Simultaneous Determination of Amphetamine, Methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in Urine By Solid-phase Extraction and GC-MS: a Method Optimized for High-volume Laboratories." Journal of Analytical Toxicology, vol. 26, no. 5, 2002, pp. 253-61.
Stout PR, Horn CK, Klette KL. Rapid simultaneous determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in urine by solid-phase extraction and GC-MS: a method optimized for high-volume laboratories. J Anal Toxicol. 2002;26(5):253-61.
Stout, P. R., Horn, C. K., & Klette, K. L. (2002). Rapid simultaneous determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in urine by solid-phase extraction and GC-MS: a method optimized for high-volume laboratories. Journal of Analytical Toxicology, 26(5), 253-61.
Stout PR, Horn CK, Klette KL. Rapid Simultaneous Determination of Amphetamine, Methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in Urine By Solid-phase Extraction and GC-MS: a Method Optimized for High-volume Laboratories. J Anal Toxicol. 2002 Jul-Aug;26(5):253-61. PubMed PMID: 12166811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid simultaneous determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 3,4-methylenedioxyethylamphetamine in urine by solid-phase extraction and GC-MS: a method optimized for high-volume laboratories. AU - Stout,Peter R, AU - Horn,Carl K, AU - Klette,Kevin L, PY - 2002/8/9/pubmed PY - 2003/1/8/medline PY - 2002/8/9/entrez SP - 253 EP - 61 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 26 IS - 5 N2 - To facilitate analysis of high sample volumes, an extraction, derivatization and gas chromatographic-mass spectrometric analysis method was developed to simultaneously determine amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA) 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) in urine. This method utilized a positive-pressure manifold cation-exchange polymer-based solid-phase extraction followed by elution directly into automated liquid sampler (ALS) vials. Rapid derivatization was accomplished using heptafluorobutyric anhydride (HFBA). Recoveries averaged 90% or greater for each of the compounds. Limits of detection were 62.5 ng/mL (AMP and MDEA), 15.6 ng/mL (MAMP), and 31.3 ng/mL (MDA and MDMA) using a 2-mL sample volume. The method was linear to 5000 ng/mL for all compounds using MDMA-d5 and MAMP-d14 as internal standards. Over 200 human urine samples previously determined to contain the target analytes were analyzed using the method. Excellent agreement was seen with previous quantitations. The method was challenged with 75 potentially interfering compounds and no interferences were seen. These interfering compounds included ephedrine, pseudoephedrine, phenylpropanolamine, and phenethylamine. The method resulted in dramatic reductions in processing time and waste production. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/12166811/Rapid_simultaneous_determination_of_amphetamine_methamphetamine_34_methylenedioxyamphetamine_34_methylenedioxymethamphetamine_and_34_methylenedioxyethylamphetamine_in_urine_by_solid_phase_extraction_and_GC_MS:_a_method_optimized_for_high_volume_laboratories_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/26.5.253 DB - PRIME DP - Unbound Medicine ER -