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Fermentation performance and intracellular metabolite patterns in laboratory and industrial xylose-fermenting Saccharomyces cerevisiae.
Appl Microbiol Biotechnol. 2002 Aug; 59(4-5):436-42.AM

Abstract

Heterologous genes for xylose utilization were introduced into an industrial Saccharomyces cerevisiae, strain A, with the aim of producing fuel ethanol from lignocellulosic feedstocks. Two transformants, A4 and A6, were evaluated by comparing the performance in 4-l anaerobic batch cultivations to both the parent strain and a laboratory xylose-utilizing strain: S. cerevisiae TMB 3001. During growth in a minimal medium containing a mixture of glucose and xylose (50 g/l each), glucose was preferentially consumed. During the first growth phase on glucose, the specific growth rates were 0.26, 0.32, 0.27 and 0.30 h(-1) for strains TMB 3001, A (parental strain), A4, and A6, respectively. The specific ethanol productivities were 0.04, 0.13, 0.04 and 0.03 g/g.per hour, for TMB 3001, A, A4 and A6, respectively. The specific xylose consumption rates were 0.06, 0.21 and 0.14 g/g.per hour, respectively for strains TMB 3001, A4 and A6. Xylose consumption resulted mainly in the formation of xylitol, with biomass and ethanol being minor products. The metabolite profile of intermediates in the pentose phosphate pathway and key glycolytic intermediates were determined during growth on glucose and xylose, respectively. The metabolite pattern differed depending on whether glucose or xylose was utilized. The levels of intracellular metabolites were higher in the industrial strains than in the laboratory strain during growth on xylose.

Authors+Show Affiliations

Center for Process Biotechnology, Biocentrum-DTU, Building 223, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12172606

Citation

Zaldivar, J, et al. "Fermentation Performance and Intracellular Metabolite Patterns in Laboratory and Industrial Xylose-fermenting Saccharomyces Cerevisiae." Applied Microbiology and Biotechnology, vol. 59, no. 4-5, 2002, pp. 436-42.
Zaldivar J, Borges A, Johansson B, et al. Fermentation performance and intracellular metabolite patterns in laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2002;59(4-5):436-42.
Zaldivar, J., Borges, A., Johansson, B., Smits, H. P., Villas-Bôas, S. G., Nielsen, J., & Olsson, L. (2002). Fermentation performance and intracellular metabolite patterns in laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 59(4-5), 436-42.
Zaldivar J, et al. Fermentation Performance and Intracellular Metabolite Patterns in Laboratory and Industrial Xylose-fermenting Saccharomyces Cerevisiae. Appl Microbiol Biotechnol. 2002;59(4-5):436-42. PubMed PMID: 12172606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fermentation performance and intracellular metabolite patterns in laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. AU - Zaldivar,J, AU - Borges,A, AU - Johansson,B, AU - Smits,H P, AU - Villas-Bôas,S G, AU - Nielsen,J, AU - Olsson,L, Y1 - 2002/07/03/ PY - 2002/03/04/received PY - 2002/05/21/revised PY - 2002/05/26/accepted PY - 2002/8/13/pubmed PY - 2002/11/1/medline PY - 2002/8/13/entrez SP - 436 EP - 42 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 59 IS - 4-5 N2 - Heterologous genes for xylose utilization were introduced into an industrial Saccharomyces cerevisiae, strain A, with the aim of producing fuel ethanol from lignocellulosic feedstocks. Two transformants, A4 and A6, were evaluated by comparing the performance in 4-l anaerobic batch cultivations to both the parent strain and a laboratory xylose-utilizing strain: S. cerevisiae TMB 3001. During growth in a minimal medium containing a mixture of glucose and xylose (50 g/l each), glucose was preferentially consumed. During the first growth phase on glucose, the specific growth rates were 0.26, 0.32, 0.27 and 0.30 h(-1) for strains TMB 3001, A (parental strain), A4, and A6, respectively. The specific ethanol productivities were 0.04, 0.13, 0.04 and 0.03 g/g.per hour, for TMB 3001, A, A4 and A6, respectively. The specific xylose consumption rates were 0.06, 0.21 and 0.14 g/g.per hour, respectively for strains TMB 3001, A4 and A6. Xylose consumption resulted mainly in the formation of xylitol, with biomass and ethanol being minor products. The metabolite profile of intermediates in the pentose phosphate pathway and key glycolytic intermediates were determined during growth on glucose and xylose, respectively. The metabolite pattern differed depending on whether glucose or xylose was utilized. The levels of intracellular metabolites were higher in the industrial strains than in the laboratory strain during growth on xylose. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/12172606/Fermentation_performance_and_intracellular_metabolite_patterns_in_laboratory_and_industrial_xylose_fermenting_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -