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Metabolic profiling of oxylipins in germinating cucumber seedlings--lipoxygenase-dependent degradation of triacylglycerols and biosynthesis of volatile aldehydes.
Planta. 2002 Aug; 215(4):612-9.P

Abstract

A particular isoform of lipoxygenase (LOX) localized on lipid bodies was shown by earlier investigations to play a role in initiating the mobilization of triacylglycerols during seed germination. Here, further physiological functions of LOXs within whole cotyledons of cucumber (Cucumis sativus L.) were analyzed by measuring the endogenous amounts of LOX-derived products. The lipid-body LOX-derived esterified (13 S)-hydroperoxy linoleic acid was the dominant metabolite of the LOX pathway in this tissue. It accumulated to about 14 micromol/g fresh weight, which represented about 6% of the total amount of linoleic acid in cotyledons. This LOX product was not only reduced to its hydroxy derivative, leading to degradation by beta-oxidation, but alternatively it was metabolized by fatty acid hydroperoxide lyase leading to formation of hexanal as well. Furthermore, the activities of LOX forms metabolizing linolenic acid were detected by measuring the accumulation of volatile aldehydes and the allene oxide synthase-derived metabolite jasmonic acid. The first evidence is presented for an involvement of a lipid-body LOX form in the production of volatile aldehydes.

Authors+Show Affiliations

Weichert H
Institute oof Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Kolbe A
No affiliation info available
Kraus A
No affiliation info available
Wasternack C
No affiliation info available
Feussner I
No affiliation info available

MeSH

AldehydesCotyledonCucumis sativusCyclopentanesFatty Acids, UnsaturatedGerminationIntramolecular OxidoreductasesIsoenzymesLinoleic AcidsLinoleic Acids, ConjugatedLipid PeroxidesLipoxygenaseOxylipinsSeedsTime FactorsTriglyceridesVolatilization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12172844

Citation

Weichert, Heiko, et al. "Metabolic Profiling of Oxylipins in Germinating Cucumber Seedlings--lipoxygenase-dependent Degradation of Triacylglycerols and Biosynthesis of Volatile Aldehydes." Planta, vol. 215, no. 4, 2002, pp. 612-9.
Weichert H, Kolbe A, Kraus A, et al. Metabolic profiling of oxylipins in germinating cucumber seedlings--lipoxygenase-dependent degradation of triacylglycerols and biosynthesis of volatile aldehydes. Planta. 2002;215(4):612-9.
Weichert, H., Kolbe, A., Kraus, A., Wasternack, C., & Feussner, I. (2002). Metabolic profiling of oxylipins in germinating cucumber seedlings--lipoxygenase-dependent degradation of triacylglycerols and biosynthesis of volatile aldehydes. Planta, 215(4), 612-9.
Weichert H, et al. Metabolic Profiling of Oxylipins in Germinating Cucumber Seedlings--lipoxygenase-dependent Degradation of Triacylglycerols and Biosynthesis of Volatile Aldehydes. Planta. 2002;215(4):612-9. PubMed PMID: 12172844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic profiling of oxylipins in germinating cucumber seedlings--lipoxygenase-dependent degradation of triacylglycerols and biosynthesis of volatile aldehydes. AU - Weichert,Heiko, AU - Kolbe,Adelheid, AU - Kraus,Angelika, AU - Wasternack,Claus, AU - Feussner,Ivo, Y1 - 2002/04/24/ PY - 2001/11/06/received PY - 2002/03/12/accepted PY - 2002/8/13/pubmed PY - 2002/12/27/medline PY - 2002/8/13/entrez SP - 612 EP - 9 JF - Planta JO - Planta VL - 215 IS - 4 N2 - A particular isoform of lipoxygenase (LOX) localized on lipid bodies was shown by earlier investigations to play a role in initiating the mobilization of triacylglycerols during seed germination. Here, further physiological functions of LOXs within whole cotyledons of cucumber (Cucumis sativus L.) were analyzed by measuring the endogenous amounts of LOX-derived products. The lipid-body LOX-derived esterified (13 S)-hydroperoxy linoleic acid was the dominant metabolite of the LOX pathway in this tissue. It accumulated to about 14 micromol/g fresh weight, which represented about 6% of the total amount of linoleic acid in cotyledons. This LOX product was not only reduced to its hydroxy derivative, leading to degradation by beta-oxidation, but alternatively it was metabolized by fatty acid hydroperoxide lyase leading to formation of hexanal as well. Furthermore, the activities of LOX forms metabolizing linolenic acid were detected by measuring the accumulation of volatile aldehydes and the allene oxide synthase-derived metabolite jasmonic acid. The first evidence is presented for an involvement of a lipid-body LOX form in the production of volatile aldehydes. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/12172844/Metabolic_profiling_of_oxylipins_in_germinating_cucumber_seedlings__lipoxygenase_dependent_degradation_of_triacylglycerols_and_biosynthesis_of_volatile_aldehydes_ DB - PRIME DP - Unbound Medicine ER -