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Isolation and characterisation of Indian Ocean ciguatoxin.
Toxicon. 2002 Jun; 40(6):685-93.T

Abstract

We report the isolation and initial characterisation of Indian Ocean ciguatoxin (I-CTX) present in toxic lipid soluble extracts isolated from ciguateric fishes collected off the Republic of Mauritius in the Indian Ocean. Following i.p. injection of this extract, mice displayed symptoms that were similar, though not identical, to those produced by Pacific and Caribbean ciguatoxins (P-CTXs and C-CTXs). Using a radiolabelled brevetoxin (PbTx) binding assay and mouse bioassay guided fractionation, I-CTX was purified by Florisil, Sephadex LH-20 and TSK HW-40S chromatography with good recovery. Isolation to purity was not possible by preparative reversed phase high-performance liquid chromatography (HPLC) due to significant losses of toxicity. However, analytical reversed phase HPLC coupled to an electrospray mass spectrometry detector identified a [M + H](+) ion at m/z 1141.58 which co-eluted with activity that displaced [3H]-PbTx binding to rat brain. This mass corresponded to C-CTX-1, but the fragmentation pattern of I-CTX showed a different ratio of pseudo molecular and product ions. I-CTX was found to elute later than P-CTX-1 but was practically indistinguishable from C-CTX-1 on reversed phase HPLC, while the TSK HW-40S column chromatography differentiated I-CTX from the later eluting C-CTX-1. Taken together, these results indicate that I-CTX is a new ciguatoxin (CTX) responsible for ciguatera caused by reef fish in the Indian Ocean.

Authors+Show Affiliations

Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, The University of Queensland, St Lucia, Brisbane, Qld 4072, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12175604

Citation

Hamilton, Brett, et al. "Isolation and Characterisation of Indian Ocean Ciguatoxin." Toxicon : Official Journal of the International Society On Toxinology, vol. 40, no. 6, 2002, pp. 685-93.
Hamilton B, Hurbungs M, Vernoux JP, et al. Isolation and characterisation of Indian Ocean ciguatoxin. Toxicon. 2002;40(6):685-93.
Hamilton, B., Hurbungs, M., Vernoux, J. P., Jones, A., & Lewis, R. J. (2002). Isolation and characterisation of Indian Ocean ciguatoxin. Toxicon : Official Journal of the International Society On Toxinology, 40(6), 685-93.
Hamilton B, et al. Isolation and Characterisation of Indian Ocean Ciguatoxin. Toxicon. 2002;40(6):685-93. PubMed PMID: 12175604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterisation of Indian Ocean ciguatoxin. AU - Hamilton,Brett, AU - Hurbungs,Mira, AU - Vernoux,Jean-Paul, AU - Jones,Alun, AU - Lewis,Richard J, PY - 2002/8/15/pubmed PY - 2002/9/13/medline PY - 2002/8/15/entrez SP - 685 EP - 93 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 40 IS - 6 N2 - We report the isolation and initial characterisation of Indian Ocean ciguatoxin (I-CTX) present in toxic lipid soluble extracts isolated from ciguateric fishes collected off the Republic of Mauritius in the Indian Ocean. Following i.p. injection of this extract, mice displayed symptoms that were similar, though not identical, to those produced by Pacific and Caribbean ciguatoxins (P-CTXs and C-CTXs). Using a radiolabelled brevetoxin (PbTx) binding assay and mouse bioassay guided fractionation, I-CTX was purified by Florisil, Sephadex LH-20 and TSK HW-40S chromatography with good recovery. Isolation to purity was not possible by preparative reversed phase high-performance liquid chromatography (HPLC) due to significant losses of toxicity. However, analytical reversed phase HPLC coupled to an electrospray mass spectrometry detector identified a [M + H](+) ion at m/z 1141.58 which co-eluted with activity that displaced [3H]-PbTx binding to rat brain. This mass corresponded to C-CTX-1, but the fragmentation pattern of I-CTX showed a different ratio of pseudo molecular and product ions. I-CTX was found to elute later than P-CTX-1 but was practically indistinguishable from C-CTX-1 on reversed phase HPLC, while the TSK HW-40S column chromatography differentiated I-CTX from the later eluting C-CTX-1. Taken together, these results indicate that I-CTX is a new ciguatoxin (CTX) responsible for ciguatera caused by reef fish in the Indian Ocean. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/12175604/Isolation_and_characterisation_of_Indian_Ocean_ciguatoxin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041010101002598 DB - PRIME DP - Unbound Medicine ER -