Tags

Type your tag names separated by a space and hit enter

Intranasal immunization with recombinant outer membrane protein P6 induces specific immune responses against nontypeable Haemophilus influenzae.
Int J Pediatr Otorhinolaryngol. 2002 Sep 02; 65(2):109-16.IJ

Abstract

OBJECTIVE

Nontypeable Haemophilus influenzae (NTHi) is one of the leading causative pathogens for otitis media. The outer membrane protein P6 of NTHi is highly conserved among the strains and is an attractive candidate for a preventive vaccine. However, for the production of a relatively small amount P6 containing lipopolysaccharides, the development of a recombinant version of this protein is required. This study was designed to investigate the specific mucosal immunity induced by intranasal immunization of recombinant P6 (rP6) with cholera toxin (CT).

METHODS

BALB/c mice were immunized with of rP6 (30 microg) and CT (2 microg) intranasally every 2 days for 2 weeks. Anti-rP6 specific IgG, IgA and IgM antibodies and the subclass of anti-rP6 specific IgG antibody were determined by enzyme linked immunosorbent assay (ELISA). Anti-rP6 specific IgA in nasopharyngeal washings were also determined by ELISA. Nasopharyngeal clearance of inoculated NTHi after the intranasal immunization were assessed. All statistical differences between the two groups were assessed by ANOVA parametric test.

RESULTS

Intranasal immunization with rP6 and CT evoked rP6-specific mucosal IgA immune response as well as the systemic IgG immune response against rP6 and enhanced nasopharyngeal clearance of inoculated live NTHi.

CONCLUSION

These results indicate the good immunogenicities of rP6 to induce specific immune responses against NTHi. Intranasal immunization with rP6 will be an effective approach to protect infections of NTHi.

Authors+Show Affiliations

Department of Otolaryngology - Head and Neck Surgery, Wakayama Medical University, Kimiidera 811-1, Wakayama-shi, Wkayama 641-0032, Japan. mhotomi@wakayama-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12176180

Citation

Hotomi, Muneki, et al. "Intranasal Immunization With Recombinant Outer Membrane Protein P6 Induces Specific Immune Responses Against Nontypeable Haemophilus Influenzae." International Journal of Pediatric Otorhinolaryngology, vol. 65, no. 2, 2002, pp. 109-16.
Hotomi M, Yamanaka N, Shimada J, et al. Intranasal immunization with recombinant outer membrane protein P6 induces specific immune responses against nontypeable Haemophilus influenzae. Int J Pediatr Otorhinolaryngol. 2002;65(2):109-16.
Hotomi, M., Yamanaka, N., Shimada, J., Suzumoto, M., Ikeda, Y., Sakai, A., Arai, J., & Green, B. (2002). Intranasal immunization with recombinant outer membrane protein P6 induces specific immune responses against nontypeable Haemophilus influenzae. International Journal of Pediatric Otorhinolaryngology, 65(2), 109-16.
Hotomi M, et al. Intranasal Immunization With Recombinant Outer Membrane Protein P6 Induces Specific Immune Responses Against Nontypeable Haemophilus Influenzae. Int J Pediatr Otorhinolaryngol. 2002 Sep 2;65(2):109-16. PubMed PMID: 12176180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intranasal immunization with recombinant outer membrane protein P6 induces specific immune responses against nontypeable Haemophilus influenzae. AU - Hotomi,Muneki, AU - Yamanaka,Noboru, AU - Shimada,Jun, AU - Suzumoto,Masaki, AU - Ikeda,Yorihiko, AU - Sakai,Akihiro, AU - Arai,Jun, AU - Green,Bruce, PY - 2002/8/15/pubmed PY - 2002/10/11/medline PY - 2002/8/15/entrez SP - 109 EP - 16 JF - International journal of pediatric otorhinolaryngology JO - Int J Pediatr Otorhinolaryngol VL - 65 IS - 2 N2 - OBJECTIVE: Nontypeable Haemophilus influenzae (NTHi) is one of the leading causative pathogens for otitis media. The outer membrane protein P6 of NTHi is highly conserved among the strains and is an attractive candidate for a preventive vaccine. However, for the production of a relatively small amount P6 containing lipopolysaccharides, the development of a recombinant version of this protein is required. This study was designed to investigate the specific mucosal immunity induced by intranasal immunization of recombinant P6 (rP6) with cholera toxin (CT). METHODS: BALB/c mice were immunized with of rP6 (30 microg) and CT (2 microg) intranasally every 2 days for 2 weeks. Anti-rP6 specific IgG, IgA and IgM antibodies and the subclass of anti-rP6 specific IgG antibody were determined by enzyme linked immunosorbent assay (ELISA). Anti-rP6 specific IgA in nasopharyngeal washings were also determined by ELISA. Nasopharyngeal clearance of inoculated NTHi after the intranasal immunization were assessed. All statistical differences between the two groups were assessed by ANOVA parametric test. RESULTS: Intranasal immunization with rP6 and CT evoked rP6-specific mucosal IgA immune response as well as the systemic IgG immune response against rP6 and enhanced nasopharyngeal clearance of inoculated live NTHi. CONCLUSION: These results indicate the good immunogenicities of rP6 to induce specific immune responses against NTHi. Intranasal immunization with rP6 will be an effective approach to protect infections of NTHi. SN - 0165-5876 UR - https://www.unboundmedicine.com/medline/citation/12176180/Intranasal_immunization_with_recombinant_outer_membrane_protein_P6_induces_specific_immune_responses_against_nontypeable_Haemophilus_influenzae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165587602000769 DB - PRIME DP - Unbound Medicine ER -