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Amplification of small analytes in polymer solution by capillary electrophoresis.
Electrophoresis. 2002 Jun; 23(11):1633-41.E

Abstract

We report concentration methods for the analysis of small solutes by capillary electrophoresis in conjunction with laser-induced native fluorescence using a Nd:YAG laser. After injecting samples, poly(ethylene oxide) (PEO) in the anode reservoir entered a capillary filled with Tris-borate buffer. When migrating in PEO solution, the analytes slowed down and stacked at the interface between the sample zone and PEO solution. As a result, the limits of detection (LODs) down to 8 pM for 2-naphthalenesulfonic acid and 70 pM for L-tryptophan have been achieved when injecting at 30 cm height for 120 s and 230 s, respectively. Such low LODs are partially due to the effects of NaCl in the samples and PEO on the fluorescence characteristics of the analytes. In addition, the concentrations of NaCl and PEO have great impacts on the migration of the analytes and electroosmotic flow, thereby affecting resolution and speed. Without pretreatment, the determinations of five important markers in urine samples and two acids in a cerebrospinal fluid sample have been performed separately, with the relative standard deviations of the concentrations less than 3.6%. Furthermore, by applying a short plug of low-pH buffer after injection, the analysis of greater volumes of the urine sample has been carried out, resulting in detecting more peaks.

Authors+Show Affiliations

Hsieh MM
Department of Chemistry, National Taiwan University, Taipei, Taiwan, R.O.C.
Hsu CE
No affiliation info available
Tseng WL
No affiliation info available
Chang HT
No affiliation info available

MeSH

Biogenic AminesElectrophoresis, CapillaryFluorescenceHumansIndolesLasersMaleOrganic ChemicalsPolyethylene GlycolsSodium ChlorideSolutions

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12179982

Citation

Hsieh, Ming-Mu, et al. "Amplification of Small Analytes in Polymer Solution By Capillary Electrophoresis." Electrophoresis, vol. 23, no. 11, 2002, pp. 1633-41.
Hsieh MM, Hsu CE, Tseng WL, et al. Amplification of small analytes in polymer solution by capillary electrophoresis. Electrophoresis. 2002;23(11):1633-41.
Hsieh, M. M., Hsu, C. E., Tseng, W. L., & Chang, H. T. (2002). Amplification of small analytes in polymer solution by capillary electrophoresis. Electrophoresis, 23(11), 1633-41.
Hsieh MM, et al. Amplification of Small Analytes in Polymer Solution By Capillary Electrophoresis. Electrophoresis. 2002;23(11):1633-41. PubMed PMID: 12179982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplification of small analytes in polymer solution by capillary electrophoresis. AU - Hsieh,Ming-Mu, AU - Hsu,Chien-En, AU - Tseng,Wei-Lung, AU - Chang,Huan-Tsung, PY - 2002/8/16/pubmed PY - 2003/7/19/medline PY - 2002/8/16/entrez SP - 1633 EP - 41 JF - Electrophoresis JO - Electrophoresis VL - 23 IS - 11 N2 - We report concentration methods for the analysis of small solutes by capillary electrophoresis in conjunction with laser-induced native fluorescence using a Nd:YAG laser. After injecting samples, poly(ethylene oxide) (PEO) in the anode reservoir entered a capillary filled with Tris-borate buffer. When migrating in PEO solution, the analytes slowed down and stacked at the interface between the sample zone and PEO solution. As a result, the limits of detection (LODs) down to 8 pM for 2-naphthalenesulfonic acid and 70 pM for L-tryptophan have been achieved when injecting at 30 cm height for 120 s and 230 s, respectively. Such low LODs are partially due to the effects of NaCl in the samples and PEO on the fluorescence characteristics of the analytes. In addition, the concentrations of NaCl and PEO have great impacts on the migration of the analytes and electroosmotic flow, thereby affecting resolution and speed. Without pretreatment, the determinations of five important markers in urine samples and two acids in a cerebrospinal fluid sample have been performed separately, with the relative standard deviations of the concentrations less than 3.6%. Furthermore, by applying a short plug of low-pH buffer after injection, the analysis of greater volumes of the urine sample has been carried out, resulting in detecting more peaks. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/12179982/Amplification_of_small_analytes_in_polymer_solution_by_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -