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Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection.
Electrophoresis. 2002 Jun; 23(11):1682-8.E

Abstract

We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.

Authors+Show Affiliations

Slais K
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, CZ-61142 Brno, Czech Republic. slais@iach.cz
Horká M
No affiliation info available
Novácková J
No affiliation info available
Friedl Z
No affiliation info available

MeSH

AnimalsElectrophoresis, CapillaryFluoresceinFluorescein-5-isothiocyanateFluorescenceFluorescent DyesHumansIsoelectric FocusingLasersOsmolar ConcentrationProteinsReference Standards

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12179988

Citation

Slais, Karel, et al. "Fluorescein-based pI Markers for Capillary Isoelectric Focusing With Laser-induced Fluorescence Detection." Electrophoresis, vol. 23, no. 11, 2002, pp. 1682-8.
Slais K, Horká M, Novácková J, et al. Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection. Electrophoresis. 2002;23(11):1682-8.
Slais, K., Horká, M., Novácková, J., & Friedl, Z. (2002). Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection. Electrophoresis, 23(11), 1682-8.
Slais K, et al. Fluorescein-based pI Markers for Capillary Isoelectric Focusing With Laser-induced Fluorescence Detection. Electrophoresis. 2002;23(11):1682-8. PubMed PMID: 12179988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection. AU - Slais,Karel, AU - Horká,Marie, AU - Novácková,Jana, AU - Friedl,Zdenek, PY - 2002/8/16/pubmed PY - 2003/7/19/medline PY - 2002/8/16/entrez SP - 1682 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 23 IS - 11 N2 - We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/12179988/Fluorescein_based_pI_markers_for_capillary_isoelectric_focusing_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -