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CD8+ T cells from feline immunodeficiency virus (FIV) infected cats suppress exogenous FIV replication of their peripheral blood mononuclear cells in vitro.
Arch Virol. 2002 Aug; 147(8):1517-29.AV

Abstract

Feline immunodeficiency virus (FIV) isolates from domestic cats have been classified into five subtypes, designated A, B, C, D and E. Although many FIV-infected cats may have frequent contact with multiple strains of FIV, they usually become infected with a single FIV subtype. In the present study, we demonstrate that peripheral blood mononuclear cells (PBMC) of FIV infected cats were resistant to exogenous FIV (second virus) replication in vitro and that the resistance of these PBMC was mediated by CD8+ T cells. In cats with a low anti-FIV activity of CD8+ T cells, the proviral DNA of the second virus inoculated into PBMC was detected intracellularly, and both the second and the originally infecting strain (original virus) were produced in the culture supernatant. In contrast, in cats with a high anti-FIV activity of CD8+ T cells, both the proviral DNA of the second virus and the original virus were detected in PBMC intracellularly, but neither virus was produced in the culture supernatant. However, when PBMCs from these cats were depleted of CD8+ T cells, the RNA of both viruses was detected in the culture supernatant. These results suggest that CD8+ T cells inhibit the late phase of FIV replication after viral integration. Moreover, the inhibition was also effective against FIV strains of different subtypes from that of the original strain. It appears that the CD8+ T cell-mediated immune response plays important roles in the maintenance of an asymptomatic state in FIV-infected cats and their resistance to superinfection.

Authors+Show Affiliations

Hohdatsu T
Department of Veterinary Infectious Diseases, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 34, Japan. hohdatsu@vmas.kitasato-u.ac.jp
Sasagawa T
No affiliation info available
Yamazaki A
No affiliation info available
Motokawa K
No affiliation info available
Kusuhara H
No affiliation info available
Kaneshima T
No affiliation info available
Koyama H
No affiliation info available

MeSH

AnimalsCD8-Positive T-LymphocytesCatsFeline Acquired Immunodeficiency SyndromeImmunodeficiency Virus, FelineLeukocytes, MononuclearPolymerase Chain ReactionVirus Replication

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12181672

Citation

Hohdatsu, T, et al. "CD8+ T Cells From Feline Immunodeficiency Virus (FIV) Infected Cats Suppress Exogenous FIV Replication of Their Peripheral Blood Mononuclear Cells in Vitro." Archives of Virology, vol. 147, no. 8, 2002, pp. 1517-29.
Hohdatsu T, Sasagawa T, Yamazaki A, et al. CD8+ T cells from feline immunodeficiency virus (FIV) infected cats suppress exogenous FIV replication of their peripheral blood mononuclear cells in vitro. Arch Virol. 2002;147(8):1517-29.
Hohdatsu, T., Sasagawa, T., Yamazaki, A., Motokawa, K., Kusuhara, H., Kaneshima, T., & Koyama, H. (2002). CD8+ T cells from feline immunodeficiency virus (FIV) infected cats suppress exogenous FIV replication of their peripheral blood mononuclear cells in vitro. Archives of Virology, 147(8), 1517-29.
Hohdatsu T, et al. CD8+ T Cells From Feline Immunodeficiency Virus (FIV) Infected Cats Suppress Exogenous FIV Replication of Their Peripheral Blood Mononuclear Cells in Vitro. Arch Virol. 2002;147(8):1517-29. PubMed PMID: 12181672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD8+ T cells from feline immunodeficiency virus (FIV) infected cats suppress exogenous FIV replication of their peripheral blood mononuclear cells in vitro. AU - Hohdatsu,T, AU - Sasagawa,T, AU - Yamazaki,A, AU - Motokawa,K, AU - Kusuhara,H, AU - Kaneshima,T, AU - Koyama,H, PY - 2002/8/16/pubmed PY - 2002/9/20/medline PY - 2002/8/16/entrez SP - 1517 EP - 29 JF - Archives of virology JO - Arch Virol VL - 147 IS - 8 N2 - Feline immunodeficiency virus (FIV) isolates from domestic cats have been classified into five subtypes, designated A, B, C, D and E. Although many FIV-infected cats may have frequent contact with multiple strains of FIV, they usually become infected with a single FIV subtype. In the present study, we demonstrate that peripheral blood mononuclear cells (PBMC) of FIV infected cats were resistant to exogenous FIV (second virus) replication in vitro and that the resistance of these PBMC was mediated by CD8+ T cells. In cats with a low anti-FIV activity of CD8+ T cells, the proviral DNA of the second virus inoculated into PBMC was detected intracellularly, and both the second and the originally infecting strain (original virus) were produced in the culture supernatant. In contrast, in cats with a high anti-FIV activity of CD8+ T cells, both the proviral DNA of the second virus and the original virus were detected in PBMC intracellularly, but neither virus was produced in the culture supernatant. However, when PBMCs from these cats were depleted of CD8+ T cells, the RNA of both viruses was detected in the culture supernatant. These results suggest that CD8+ T cells inhibit the late phase of FIV replication after viral integration. Moreover, the inhibition was also effective against FIV strains of different subtypes from that of the original strain. It appears that the CD8+ T cell-mediated immune response plays important roles in the maintenance of an asymptomatic state in FIV-infected cats and their resistance to superinfection. SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/12181672/CD8+_T_cells_from_feline_immunodeficiency_virus__FIV__infected_cats_suppress_exogenous_FIV_replication_of_their_peripheral_blood_mononuclear_cells_in_vitro_ DB - PRIME DP - Unbound Medicine ER -