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Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer.
Genes Dev. 2002 Aug 15; 16(16):2085-96.GD

Abstract

Saccharomyces mating-type switching results from replacement by gene conversion of the MAT locus with sequences copied from one of two unexpressed donor loci, HML or HMR. MATa cells recombine with HMLalpha approximately 90% of the time, whereas MATalpha cells choose HMRa 80%-90% of the time. HML preference in MATa is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between S. cerevisiae, S. carlsbergensis, and S. bayanus defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of HML. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of FKH1 significantly reduces MATa's use of HML, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates MATa donor preference through direct interaction with RE.

Authors+Show Affiliations

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9910, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12183363

Citation

Sun, Kaiming, et al. "Saccharomyces Forkhead Protein Fkh1 Regulates Donor Preference During Mating-type Switching Through the Recombination Enhancer." Genes & Development, vol. 16, no. 16, 2002, pp. 2085-96.
Sun K, Coïc E, Zhou Z, et al. Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer. Genes Dev. 2002;16(16):2085-96.
Sun, K., Coïc, E., Zhou, Z., Durrens, P., & Haber, J. E. (2002). Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer. Genes & Development, 16(16), 2085-96.
Sun K, et al. Saccharomyces Forkhead Protein Fkh1 Regulates Donor Preference During Mating-type Switching Through the Recombination Enhancer. Genes Dev. 2002 Aug 15;16(16):2085-96. PubMed PMID: 12183363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer. AU - Sun,Kaiming, AU - Coïc,Eric, AU - Zhou,Zhiqi, AU - Durrens,Pascal, AU - Haber,James E, PY - 2002/8/17/pubmed PY - 2002/9/25/medline PY - 2002/8/17/entrez SP - 2085 EP - 96 JF - Genes & development JO - Genes Dev VL - 16 IS - 16 N2 - Saccharomyces mating-type switching results from replacement by gene conversion of the MAT locus with sequences copied from one of two unexpressed donor loci, HML or HMR. MATa cells recombine with HMLalpha approximately 90% of the time, whereas MATalpha cells choose HMRa 80%-90% of the time. HML preference in MATa is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between S. cerevisiae, S. carlsbergensis, and S. bayanus defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of HML. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of FKH1 significantly reduces MATa's use of HML, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates MATa donor preference through direct interaction with RE. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/12183363/Saccharomyces_forkhead_protein_Fkh1_regulates_donor_preference_during_mating_type_switching_through_the_recombination_enhancer_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=12183363 DB - PRIME DP - Unbound Medicine ER -