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ELISA of anti-endomysial antibodies in the diagnosis of celiac disease: comparison with immunofluorescence assay of anti-endomysial antibodies and tissue transglutaminase antibodies.
Isr Med Assoc J 2002; 4(8):594-6IM

Abstract

BACKGROUND

Celiac disease is common in both children and adults. Small intestinal biopsy is mandatory for establishing a diagnosis. Anti-endomysial antibodies, detected by immunofluorescence, have a sensitivity and specificity close to 100% in the diagnosis of CD. Recently, tissue transglutaminase has been identified as the target autoantigen of antibodies against endomysium, and TTG antibodies are comparable to EMA-IMF in the diagnosis of CD.

OBJECTIVE

To evaluate a new enzyme-linked immunosorbent assay kit for EMA, compared to EMA-IMF and TTG antibodies in the diagnosis of CD.

METHODS

Our study population included all subjects with positive EMA-IMF who underwent intestinal biopsy (n = 21). From the same sera, TTG antibodies and EMA-ELISA were determined, and all antibody results were compared to the biopsy findings.

RESULTS

EMA-IMF was able to predict biopsy findings of CD in 19 of 21 cases (90.5%). When patients with biopsy findings compatible with CD and positive EMA-IMF (n = 19) were tested for EMA-ELISA and TTG antibodies, 18 of the 19 were positive for both EMA-ELISA and TTG antibodies. A significant correlation was found between EMA-ELISA and TTG antibody titers (r = 0.74, P < 0.001).

CONCLUSIONS

Our study demonstrates that EMA-ELISA is comparable to TTG antibodies in the diagnosis of CD, and supports the use of EMA-ELISA as a serologic marker for this disease.

Authors+Show Affiliations

Division of Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Rambam Medical Center, Haifa, Israel. shamirr@netvision.net.ilNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Studies
Journal Article

Language

eng

PubMed ID

12183862

Citation

Shamir, Raanan, et al. "ELISA of Anti-endomysial Antibodies in the Diagnosis of Celiac Disease: Comparison With Immunofluorescence Assay of Anti-endomysial Antibodies and Tissue Transglutaminase Antibodies." The Israel Medical Association Journal : IMAJ, vol. 4, no. 8, 2002, pp. 594-6.
Shamir R, Eliakim R, Lahat N, et al. ELISA of anti-endomysial antibodies in the diagnosis of celiac disease: comparison with immunofluorescence assay of anti-endomysial antibodies and tissue transglutaminase antibodies. Isr Med Assoc J. 2002;4(8):594-6.
Shamir, R., Eliakim, R., Lahat, N., Sobel, E., & Lerner, A. (2002). ELISA of anti-endomysial antibodies in the diagnosis of celiac disease: comparison with immunofluorescence assay of anti-endomysial antibodies and tissue transglutaminase antibodies. The Israel Medical Association Journal : IMAJ, 4(8), pp. 594-6.
Shamir R, et al. ELISA of Anti-endomysial Antibodies in the Diagnosis of Celiac Disease: Comparison With Immunofluorescence Assay of Anti-endomysial Antibodies and Tissue Transglutaminase Antibodies. Isr Med Assoc J. 2002;4(8):594-6. PubMed PMID: 12183862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ELISA of anti-endomysial antibodies in the diagnosis of celiac disease: comparison with immunofluorescence assay of anti-endomysial antibodies and tissue transglutaminase antibodies. AU - Shamir,Raanan, AU - Eliakim,Rami, AU - Lahat,Nitza, AU - Sobel,Esther, AU - Lerner,Aaron, PY - 2002/8/20/pubmed PY - 2002/8/31/medline PY - 2002/8/20/entrez SP - 594 EP - 6 JF - The Israel Medical Association journal : IMAJ JO - Isr. Med. Assoc. J. VL - 4 IS - 8 N2 - BACKGROUND: Celiac disease is common in both children and adults. Small intestinal biopsy is mandatory for establishing a diagnosis. Anti-endomysial antibodies, detected by immunofluorescence, have a sensitivity and specificity close to 100% in the diagnosis of CD. Recently, tissue transglutaminase has been identified as the target autoantigen of antibodies against endomysium, and TTG antibodies are comparable to EMA-IMF in the diagnosis of CD. OBJECTIVE: To evaluate a new enzyme-linked immunosorbent assay kit for EMA, compared to EMA-IMF and TTG antibodies in the diagnosis of CD. METHODS: Our study population included all subjects with positive EMA-IMF who underwent intestinal biopsy (n = 21). From the same sera, TTG antibodies and EMA-ELISA were determined, and all antibody results were compared to the biopsy findings. RESULTS: EMA-IMF was able to predict biopsy findings of CD in 19 of 21 cases (90.5%). When patients with biopsy findings compatible with CD and positive EMA-IMF (n = 19) were tested for EMA-ELISA and TTG antibodies, 18 of the 19 were positive for both EMA-ELISA and TTG antibodies. A significant correlation was found between EMA-ELISA and TTG antibody titers (r = 0.74, P < 0.001). CONCLUSIONS: Our study demonstrates that EMA-ELISA is comparable to TTG antibodies in the diagnosis of CD, and supports the use of EMA-ELISA as a serologic marker for this disease. SN - 1565-1088 UR - https://www.unboundmedicine.com/medline/citation/12183862/ELISA_of_anti_endomysial_antibodies_in_the_diagnosis_of_celiac_disease:_comparison_with_immunofluorescence_assay_of_anti_endomysial_antibodies_and_tissue_transglutaminase_antibodies_ L2 - http://www.ima.org.il/IMAJ/ViewArticle.aspx?year=2002&amp;month=08&amp;page=594 DB - PRIME DP - Unbound Medicine ER -