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High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography.
Phytochem Anal. 2002 Jul-Aug; 13(4):235-41.PA

Abstract

An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and quantified by an external standard method. In addition, each constituent was collected separately and identified by EIMS. The method has been used to analyse 30 samples of mate infusions prepared at 30 and 75 degrees C with milled leaves and stems of 14 commercial brands which had been subjected to different elaboration processes. Suspended powdered material of each infusion was also analysed after three sieving steps. There was a remarkable difference in the relative xanthine composition of the finely suspended material, the amount of which varied according to the yerba mate brand, the elaboration process and the temperature of the infusion. The importance of these results with respect to gastrointestinal disorders which have been observed by habitual consumers of mate are discussed.

Authors+Show Affiliations

PROPLAME-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina. abpomilio@sinectis.com.arNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12184178

Citation

Pomilio, Alicia B., et al. "High-performance Capillary Electrophoresis Analysis of Mate Infusions Prepared From Stems and Leaves of Ilex Paraguariensis Using Automated Micellar Electrokinetic Capillary Chromatography." Phytochemical Analysis : PCA, vol. 13, no. 4, 2002, pp. 235-41.
Pomilio AB, Trajtemberg S, Vitale AA. High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochem Anal. 2002;13(4):235-41.
Pomilio, A. B., Trajtemberg, S., & Vitale, A. A. (2002). High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochemical Analysis : PCA, 13(4), 235-41.
Pomilio AB, Trajtemberg S, Vitale AA. High-performance Capillary Electrophoresis Analysis of Mate Infusions Prepared From Stems and Leaves of Ilex Paraguariensis Using Automated Micellar Electrokinetic Capillary Chromatography. Phytochem Anal. 2002 Jul-Aug;13(4):235-41. PubMed PMID: 12184178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. AU - Pomilio,Alicia B, AU - Trajtemberg,Silvia, AU - Vitale,Arturo A, PY - 2002/8/20/pubmed PY - 2003/1/17/medline PY - 2002/8/20/entrez SP - 235 EP - 41 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 13 IS - 4 N2 - An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and quantified by an external standard method. In addition, each constituent was collected separately and identified by EIMS. The method has been used to analyse 30 samples of mate infusions prepared at 30 and 75 degrees C with milled leaves and stems of 14 commercial brands which had been subjected to different elaboration processes. Suspended powdered material of each infusion was also analysed after three sieving steps. There was a remarkable difference in the relative xanthine composition of the finely suspended material, the amount of which varied according to the yerba mate brand, the elaboration process and the temperature of the infusion. The importance of these results with respect to gastrointestinal disorders which have been observed by habitual consumers of mate are discussed. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/12184178/High_performance_capillary_electrophoresis_analysis_of_mate_infusions_prepared_from_stems_and_leaves_of_Ilex_paraguariensis_using_automated_micellar_electrokinetic_capillary_chromatography_ L2 - https://doi.org/10.1002/pca.647 DB - PRIME DP - Unbound Medicine ER -