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A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue.
Sheng Wu Gong Cheng Xue Bao. 2002 May; 18(3):286-90.SW

Abstract

The transgenic mice that express Cre recombinase in a tissue specific manner is a powerful tool in generating the conditional gene knockout mice. The rat insulin promoter was cloned target the expression of Cre in pancreatic tissue. The Cre gene was modified by adding the nuclear localization signal and the sequence for initiation by eukaryotic ribosomes at 5' terminal of the Cre gene. Cre gene was linked to the intron of human growth factor gene. This construct was introduced into the mouse eggs using microinjection. Seven mice were identified as founders carrying the Cre gene by PCR. The results of RT-PCR showed that the transgenic mouse from one founder could transcribe the foreign gene in pancreas. The Southern blot analysis indicated that the Cre recombinase expressed in pancreas of the transgenic mouse was functional.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12192858

Citation

Zhou, Jiang, et al. "A Transgenic Mouse That Targets the Expression of Cre Recombinase in Pancreatic Tissue." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 18, no. 3, 2002, pp. 286-90.
Zhou J, Cheng X, Lu YX, et al. A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. Sheng Wu Gong Cheng Xue Bao. 2002;18(3):286-90.
Zhou, J., Cheng, X., Lu, Y. X., Huang, C. F., & Yang, X. (2002). A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 18(3), 286-90.
Zhou J, et al. A Transgenic Mouse That Targets the Expression of Cre Recombinase in Pancreatic Tissue. Sheng Wu Gong Cheng Xue Bao. 2002;18(3):286-90. PubMed PMID: 12192858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. AU - Zhou,Jiang, AU - Cheng,Xuan, AU - Lu,Ya-Xin, AU - Huang,Cui-Fen, AU - Yang,Xiao, PY - 2002/8/24/pubmed PY - 2002/9/11/medline PY - 2002/8/24/entrez SP - 286 EP - 90 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 18 IS - 3 N2 - The transgenic mice that express Cre recombinase in a tissue specific manner is a powerful tool in generating the conditional gene knockout mice. The rat insulin promoter was cloned target the expression of Cre in pancreatic tissue. The Cre gene was modified by adding the nuclear localization signal and the sequence for initiation by eukaryotic ribosomes at 5' terminal of the Cre gene. Cre gene was linked to the intron of human growth factor gene. This construct was introduced into the mouse eggs using microinjection. Seven mice were identified as founders carrying the Cre gene by PCR. The results of RT-PCR showed that the transgenic mouse from one founder could transcribe the foreign gene in pancreas. The Southern blot analysis indicated that the Cre recombinase expressed in pancreas of the transgenic mouse was functional. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/12192858/A_transgenic_mouse_that_targets_the_expression_of_Cre_recombinase_in_pancreatic_tissue_ DB - PRIME DP - Unbound Medicine ER -