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Drug testing in blood: validated negative-ion chemical ionization gas chromatographic-mass spectrometric assay for determination of amphetamine and methamphetamine enantiomers and its application to toxicology cases.
Clin Chem. 2002 Sep; 48(9):1472-85.CC

Abstract

BACKGROUND

Enantioselective analysis of amphetamine (AM) or methamphetamine (MA) in urine is already a well-established tool for differentiation of illicit from therapeutic ingestion of AM or MA derivatives. However, because of the increasing importance of plasma or serum in analytical toxicology, a method for enantioselective analysis of AM and MA in these matrices is needed.

METHODS

AM and/or MA were extracted from 0.2 mL of blood plasma or serum by mixed-mode solid-phase extraction. After derivatization with S-(-)-heptafluorobutyrylprolyl chloride, the resulting diastereomers were separated by gas chromatography on a HP-5MS column during a 15-min program and detected by mass spectrometry in the negative-ion chemical ionization mode (NICI-GC-MS). The method was fully validated and applied to >50 samples from authentic toxicology cases.

RESULTS

The derivatized AM and MA enantiomers were well separated and sensitively detected. The method was linear from 5 to 250 micro g/L per enantiomer with analytical recoveries, accuracy, and within- and between-run precision well within required limits. Extraction yields were 88.9-98.6%. Implications of concentrations and enantiomeric composition of AM and MA in the authentic samples were considered.

CONCLUSIONS

This sensitive, reliable, rapid NICI-GC-MS assay is suitable for enantioselective determination of AM and MA in blood plasma or serum samples.

Authors+Show Affiliations

Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, D-66421 Homburg (Saar), Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12194924

Citation

Peters, Frank T., et al. "Drug Testing in Blood: Validated Negative-ion Chemical Ionization Gas Chromatographic-mass Spectrometric Assay for Determination of Amphetamine and Methamphetamine Enantiomers and Its Application to Toxicology Cases." Clinical Chemistry, vol. 48, no. 9, 2002, pp. 1472-85.
Peters FT, Kraemer T, Maurer HH. Drug testing in blood: validated negative-ion chemical ionization gas chromatographic-mass spectrometric assay for determination of amphetamine and methamphetamine enantiomers and its application to toxicology cases. Clin Chem. 2002;48(9):1472-85.
Peters, F. T., Kraemer, T., & Maurer, H. H. (2002). Drug testing in blood: validated negative-ion chemical ionization gas chromatographic-mass spectrometric assay for determination of amphetamine and methamphetamine enantiomers and its application to toxicology cases. Clinical Chemistry, 48(9), 1472-85.
Peters FT, Kraemer T, Maurer HH. Drug Testing in Blood: Validated Negative-ion Chemical Ionization Gas Chromatographic-mass Spectrometric Assay for Determination of Amphetamine and Methamphetamine Enantiomers and Its Application to Toxicology Cases. Clin Chem. 2002;48(9):1472-85. PubMed PMID: 12194924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Drug testing in blood: validated negative-ion chemical ionization gas chromatographic-mass spectrometric assay for determination of amphetamine and methamphetamine enantiomers and its application to toxicology cases. AU - Peters,Frank T, AU - Kraemer,Thomas, AU - Maurer,Hans H, PY - 2002/8/27/pubmed PY - 2002/9/14/medline PY - 2002/8/27/entrez SP - 1472 EP - 85 JF - Clinical chemistry JO - Clin. Chem. VL - 48 IS - 9 N2 - BACKGROUND: Enantioselective analysis of amphetamine (AM) or methamphetamine (MA) in urine is already a well-established tool for differentiation of illicit from therapeutic ingestion of AM or MA derivatives. However, because of the increasing importance of plasma or serum in analytical toxicology, a method for enantioselective analysis of AM and MA in these matrices is needed. METHODS: AM and/or MA were extracted from 0.2 mL of blood plasma or serum by mixed-mode solid-phase extraction. After derivatization with S-(-)-heptafluorobutyrylprolyl chloride, the resulting diastereomers were separated by gas chromatography on a HP-5MS column during a 15-min program and detected by mass spectrometry in the negative-ion chemical ionization mode (NICI-GC-MS). The method was fully validated and applied to >50 samples from authentic toxicology cases. RESULTS: The derivatized AM and MA enantiomers were well separated and sensitively detected. The method was linear from 5 to 250 micro g/L per enantiomer with analytical recoveries, accuracy, and within- and between-run precision well within required limits. Extraction yields were 88.9-98.6%. Implications of concentrations and enantiomeric composition of AM and MA in the authentic samples were considered. CONCLUSIONS: This sensitive, reliable, rapid NICI-GC-MS assay is suitable for enantioselective determination of AM and MA in blood plasma or serum samples. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/12194924/Drug_testing_in_blood:_validated_negative_ion_chemical_ionization_gas_chromatographic_mass_spectrometric_assay_for_determination_of_amphetamine_and_methamphetamine_enantiomers_and_its_application_to_toxicology_cases_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12194924.ui DB - PRIME DP - Unbound Medicine ER -