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A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group.
EMBO J 2002; 21(17):4550-9EJ

Abstract

PlcR is a pleiotropic regulator that activates the expression of genes encoding various virulence factors, such as phospholipases C, proteases and hemolysins, in Bacillus thuringiensis and Bacillus cereus. Here we show that the activation mechanism is under the control of a small peptide: PapR. The papR gene belongs to the PlcR regulon and is located 70 bp downstream from plcR. It encodes a 48-amino-acid peptide. Disruption of the papR gene abolished expression of the PlcR regulon, resulting in a large decrease in hemolysis and virulence in insect larvae. We demonstrated that the PapR polypeptide was secreted, then reimported via the oligopeptide permease Opp. Once inside the cell, a processed form of PapR, presumably a pentapeptide, activated the PlcR regulon by allowing PlcR to bind to its DNA target. This activating mechanism was found to be strain specific, with this specificity determined by the first residue of the penta peptide.

Authors+Show Affiliations

Unité de Biochimie Microbienne, CNRS (URA2172), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, France. lslamti@pasteur.frNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12198157

Citation

Slamti, Leyla, and Didier Lereclus. "A Cell-cell Signaling Peptide Activates the PlcR Virulence Regulon in Bacteria of the Bacillus Cereus Group." The EMBO Journal, vol. 21, no. 17, 2002, pp. 4550-9.
Slamti L, Lereclus D. A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. EMBO J. 2002;21(17):4550-9.
Slamti, L., & Lereclus, D. (2002). A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. The EMBO Journal, 21(17), pp. 4550-9.
Slamti L, Lereclus D. A Cell-cell Signaling Peptide Activates the PlcR Virulence Regulon in Bacteria of the Bacillus Cereus Group. EMBO J. 2002 Sep 2;21(17):4550-9. PubMed PMID: 12198157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. AU - Slamti,Leyla, AU - Lereclus,Didier, PY - 2002/8/29/pubmed PY - 2002/10/9/medline PY - 2002/8/29/entrez SP - 4550 EP - 9 JF - The EMBO journal JO - EMBO J. VL - 21 IS - 17 N2 - PlcR is a pleiotropic regulator that activates the expression of genes encoding various virulence factors, such as phospholipases C, proteases and hemolysins, in Bacillus thuringiensis and Bacillus cereus. Here we show that the activation mechanism is under the control of a small peptide: PapR. The papR gene belongs to the PlcR regulon and is located 70 bp downstream from plcR. It encodes a 48-amino-acid peptide. Disruption of the papR gene abolished expression of the PlcR regulon, resulting in a large decrease in hemolysis and virulence in insect larvae. We demonstrated that the PapR polypeptide was secreted, then reimported via the oligopeptide permease Opp. Once inside the cell, a processed form of PapR, presumably a pentapeptide, activated the PlcR regulon by allowing PlcR to bind to its DNA target. This activating mechanism was found to be strain specific, with this specificity determined by the first residue of the penta peptide. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/12198157/A_cell_cell_signaling_peptide_activates_the_PlcR_virulence_regulon_in_bacteria_of_the_Bacillus_cereus_group_ L2 - https://doi.org/10.1093/emboj/cdf450 DB - PRIME DP - Unbound Medicine ER -