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[RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album].
Yi Chuan Xue Bao. 2002; 29(8):719-22.YC

Abstract

A total of 520 10-mer random primers were used to identify the RAPD markers linked to the Yr5 gene between the near-isogenic line Yr5/6 x Avocet S and recurrent parent Avocet S. Three polymorphic DNA fragments, S1496(761), S1453(880) and S1418(1950), were found linked to the Yr5 gene. In which the genetic distance between S1496(761) and Yr5 gene was 2.7 cM. The fragment S1496(761) was recovered from the gel and cloned and sequenced. A pair of specific PCR primers was designed based on the sequence. The specific primers amplified the same fragment about 761bp as the random primer S1496 did. Because the primers could amplify another non-specific fragment, the PCR products must be analyzed by electrophoresis on polyacrylamide gels.

Authors+Show Affiliations

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12200864

Citation

Zhong, Ming, et al. "[RAPD Markers Linked to the Stripe Rust Resistance Gene Yr5 in the Wheat Variety Triticum Spelta Album]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 29, no. 8, 2002, pp. 719-22.
Zhong M, Niu YC, Xu SC, et al. [RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album]. Yi Chuan Xue Bao. 2002;29(8):719-22.
Zhong, M., Niu, Y. C., Xu, S. C., & Wu, L. R. (2002). [RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album]. Yi Chuan Xue Bao = Acta Genetica Sinica, 29(8), 719-22.
Zhong M, et al. [RAPD Markers Linked to the Stripe Rust Resistance Gene Yr5 in the Wheat Variety Triticum Spelta Album]. Yi Chuan Xue Bao. 2002;29(8):719-22. PubMed PMID: 12200864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album]. AU - Zhong,Ming, AU - Niu,Yong-Chun, AU - Xu,Shi-Chang, AU - Wu,Li-Ren, PY - 2002/8/31/pubmed PY - 2002/9/11/medline PY - 2002/8/31/entrez SP - 719 EP - 22 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 29 IS - 8 N2 - A total of 520 10-mer random primers were used to identify the RAPD markers linked to the Yr5 gene between the near-isogenic line Yr5/6 x Avocet S and recurrent parent Avocet S. Three polymorphic DNA fragments, S1496(761), S1453(880) and S1418(1950), were found linked to the Yr5 gene. In which the genetic distance between S1496(761) and Yr5 gene was 2.7 cM. The fragment S1496(761) was recovered from the gel and cloned and sequenced. A pair of specific PCR primers was designed based on the sequence. The specific primers amplified the same fragment about 761bp as the random primer S1496 did. Because the primers could amplify another non-specific fragment, the PCR products must be analyzed by electrophoresis on polyacrylamide gels. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/12200864/[RAPD_markers_linked_to_the_stripe_rust_resistance_gene_Yr5_in_the_wheat_variety_Triticum_spelta_album]_ DB - PRIME DP - Unbound Medicine ER -