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Retinal pigment epithelial cell distribution in central retina of rhesus monkeys.
Invest Ophthalmol Vis Sci. 2002 Sep; 43(9):2815-8.IO

Abstract

PURPOSE

To determine the cell density profile of the retinal pigment epithelium (RPE) in the central retina and relate it to the distribution of photoreceptors.

METHODS

Wholemounts of rhesus monkey (Macaca mulatta) retinas with the choroid removed but the RPE attached were stained with the nuclear stain 4',6-diamidine-2-phenylindole dihydrochloride (DAPI) and imaged with a fluorescence microscope. RPE cell nuclei were counted at the foveal center and at 0.4-mm intervals along the vertical meridian. The number of photoreceptors per RPE cell at each location was estimated by using previously published data on the distribution of rhesus photoreceptors.

RESULTS

Data were collected from eight retinas. Mean RPE cell density increased from a relatively stable baseline of approximately 4000 RPE cells/mm(2) beyond 2 mm (10 degrees) eccentricity to more than 7000 cells/mm(2) at the center of the fovea. The number of cones per RPE cell in the rhesus retina was approximately 20:1 in the foveal center (similar to the human retina) and only approximately 1.5:1 in the parafovea. However, when the rods were included, and the total number of photoreceptors per RPE cell were considered, the ratio of photoreceptors to RPE cells was lower in the fovea than in the remainder of the central retina.

CONCLUSIONS

In spite of the high cone density, there is a relatively low number of photoreceptors per RPE cell in the fovea. This may limit the metabolic demands on foveal RPE cells and help to preserve their functions in the face of aging and disease.

Authors+Show Affiliations

Schepens Eye Research Institute, Boston, Massachusetts 02114, USA. maxs@vision.eri.harvard.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12202496

Citation

Snodderly, D Max, et al. "Retinal Pigment Epithelial Cell Distribution in Central Retina of Rhesus Monkeys." Investigative Ophthalmology & Visual Science, vol. 43, no. 9, 2002, pp. 2815-8.
Snodderly DM, Sandstrom MM, Leung IY, et al. Retinal pigment epithelial cell distribution in central retina of rhesus monkeys. Invest Ophthalmol Vis Sci. 2002;43(9):2815-8.
Snodderly, D. M., Sandstrom, M. M., Leung, I. Y., Zucker, C. L., & Neuringer, M. (2002). Retinal pigment epithelial cell distribution in central retina of rhesus monkeys. Investigative Ophthalmology & Visual Science, 43(9), 2815-8.
Snodderly DM, et al. Retinal Pigment Epithelial Cell Distribution in Central Retina of Rhesus Monkeys. Invest Ophthalmol Vis Sci. 2002;43(9):2815-8. PubMed PMID: 12202496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Retinal pigment epithelial cell distribution in central retina of rhesus monkeys. AU - Snodderly,D Max, AU - Sandstrom,Marita M, AU - Leung,Ivan Y-F, AU - Zucker,Charles L, AU - Neuringer,Martha, PY - 2002/8/31/pubmed PY - 2002/9/13/medline PY - 2002/8/31/entrez SP - 2815 EP - 8 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 43 IS - 9 N2 - PURPOSE: To determine the cell density profile of the retinal pigment epithelium (RPE) in the central retina and relate it to the distribution of photoreceptors. METHODS: Wholemounts of rhesus monkey (Macaca mulatta) retinas with the choroid removed but the RPE attached were stained with the nuclear stain 4',6-diamidine-2-phenylindole dihydrochloride (DAPI) and imaged with a fluorescence microscope. RPE cell nuclei were counted at the foveal center and at 0.4-mm intervals along the vertical meridian. The number of photoreceptors per RPE cell at each location was estimated by using previously published data on the distribution of rhesus photoreceptors. RESULTS: Data were collected from eight retinas. Mean RPE cell density increased from a relatively stable baseline of approximately 4000 RPE cells/mm(2) beyond 2 mm (10 degrees) eccentricity to more than 7000 cells/mm(2) at the center of the fovea. The number of cones per RPE cell in the rhesus retina was approximately 20:1 in the foveal center (similar to the human retina) and only approximately 1.5:1 in the parafovea. However, when the rods were included, and the total number of photoreceptors per RPE cell were considered, the ratio of photoreceptors to RPE cells was lower in the fovea than in the remainder of the central retina. CONCLUSIONS: In spite of the high cone density, there is a relatively low number of photoreceptors per RPE cell in the fovea. This may limit the metabolic demands on foveal RPE cells and help to preserve their functions in the face of aging and disease. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/12202496/Retinal_pigment_epithelial_cell_distribution_in_central_retina_of_rhesus_monkeys_ L2 - https://iovs.arvojournals.org/article.aspx?volume=43&issue=9&page=2815 DB - PRIME DP - Unbound Medicine ER -