[A study on chemical composition of Gardenia sootepensis Hutch--quantitative determination of iridoid compounds by RP-HPLC].Zhongguo Zhong Yao Za Zhi. 1999 Oct; 24(10):616-8, 639.ZZ
To determine the iridoid contents in Gardenia sootepensis.
Using RP-HPLC with geniposidic acid, geniposide and scandoside methyl ether as standards.
According to the selected chromatographic conditions, the linear ranges of geniposidic acid, geniposide and scandoside methyl ether were 0.097-0.606 microgram, 0.0638-3.990 micrograms and 0.198-1.239 micrograms, respectively. The average recoveries of these three compounds were 100.0%, 99.9% and 100.1%, and RSD 1.23%, 1.38% and 1.53% (n = 5) respectively.
The method is easy, rapid, and reproducible.