Tags

Type your tag names separated by a space and hit enter

Polyadenylation can regulate ColE1 type plasmid copy number independently of any effect on RNAI decay by decreasing the interaction of antisense RNAI with its RNAII target.
Plasmid 2002; 48(1):49-58P

Abstract

Replication of ColE1-type plasmids is regulated by RNAI, an antisense RNA that interacts with the replication pre-primer, RNAII. Exonucleolytic attack at the 3' end of RNAI is impeded in pcnB mutant bacteria, which lack poly(A) polymerase I-the principal RNA polyadenylase of E. coli; this leads to accumulation of an RNAI decay intermediate (RNAI(-5)) and dramatic reduction of the plasmid copy number. Here, we report that polyadenylation can also affect RNAI-mediated control of plasmid DNA replication by inhibiting interaction of RNAI(-5) with RNAII. We show that mutation of the host pcnB gene profoundly affects the plasmid copy number, even under experimental conditions that limit the effects of polyadenylation on RNAI(-5) decay. Moreover, poly(A) tails interfere with RNAI/RNAII interaction in vitro without producing any detectable alteration of RNAI secondary structure. Our results establish the existence of a previously undetected mechanism by which RNA polyadenylation can control plasmid copy number.

Authors+Show Affiliations

Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12206755

Citation

Xu, Feng-Feng, et al. "Polyadenylation Can Regulate ColE1 Type Plasmid Copy Number Independently of Any Effect On RNAI Decay By Decreasing the Interaction of Antisense RNAI With Its RNAII Target." Plasmid, vol. 48, no. 1, 2002, pp. 49-58.
Xu FF, Gaggero C, Cohen SN. Polyadenylation can regulate ColE1 type plasmid copy number independently of any effect on RNAI decay by decreasing the interaction of antisense RNAI with its RNAII target. Plasmid. 2002;48(1):49-58.
Xu, F. F., Gaggero, C., & Cohen, S. N. (2002). Polyadenylation can regulate ColE1 type plasmid copy number independently of any effect on RNAI decay by decreasing the interaction of antisense RNAI with its RNAII target. Plasmid, 48(1), pp. 49-58.
Xu FF, Gaggero C, Cohen SN. Polyadenylation Can Regulate ColE1 Type Plasmid Copy Number Independently of Any Effect On RNAI Decay By Decreasing the Interaction of Antisense RNAI With Its RNAII Target. Plasmid. 2002;48(1):49-58. PubMed PMID: 12206755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyadenylation can regulate ColE1 type plasmid copy number independently of any effect on RNAI decay by decreasing the interaction of antisense RNAI with its RNAII target. AU - Xu,Feng-Feng, AU - Gaggero,Carina, AU - Cohen,Stanley N, PY - 2002/9/11/pubmed PY - 2002/12/5/medline PY - 2002/9/11/entrez SP - 49 EP - 58 JF - Plasmid JO - Plasmid VL - 48 IS - 1 N2 - Replication of ColE1-type plasmids is regulated by RNAI, an antisense RNA that interacts with the replication pre-primer, RNAII. Exonucleolytic attack at the 3' end of RNAI is impeded in pcnB mutant bacteria, which lack poly(A) polymerase I-the principal RNA polyadenylase of E. coli; this leads to accumulation of an RNAI decay intermediate (RNAI(-5)) and dramatic reduction of the plasmid copy number. Here, we report that polyadenylation can also affect RNAI-mediated control of plasmid DNA replication by inhibiting interaction of RNAI(-5) with RNAII. We show that mutation of the host pcnB gene profoundly affects the plasmid copy number, even under experimental conditions that limit the effects of polyadenylation on RNAI(-5) decay. Moreover, poly(A) tails interfere with RNAI/RNAII interaction in vitro without producing any detectable alteration of RNAI secondary structure. Our results establish the existence of a previously undetected mechanism by which RNA polyadenylation can control plasmid copy number. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/12206755/Polyadenylation_can_regulate_ColE1_type_plasmid_copy_number_independently_of_any_effect_on_RNAI_decay_by_decreasing_the_interaction_of_antisense_RNAI_with_its_RNAII_target_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147619X02000239 DB - PRIME DP - Unbound Medicine ER -