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Activated CD8+ T lymphocytes induce differentiation of monocytes to dendritic cells and restore the stimulatory capacity of interleukin 10-treated antigen-presenting cells.
Cancer Res. 2002 Sep 01; 62(17):5065-8.CR

Abstract

We analyzed the role of CD8+ T cells on dendritic cells (DCs). Incubation of monocytes with activated but not resting CD8+ T cells induced their differentiation into DCs with a CD1a+ and CD14- phenotype. Activated CD8+ lymphocytes induced down-regulation of CD14 expression in interleukin (IL)-10-treated macrophages and resulted in the expression of DC-related CD1a and up-regulation of mRNA transcripts for RelB, IL-12p40, MCP-2, MIP-1alpha, and ELC. These DCs were potent stimulatory cells in mixed lymphocyte reaction and induced primary CTL responses. This interaction was independent of a direct cell-to-cell contact. Monoclonal antibodies against IFN-gamma completely abolished the CD1a induction on IL-10-treated antigen-presenting cells. These results demonstrate that CD8+ cells can provide stimulatory signals to antigen-presenting cells to induce their differentiation in DC and to increase their immunostimulatory capacity.

Authors+Show Affiliations

University of Tübingen, Department of Hematology, Oncology and Immunology, D-72076 Tübingen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12208762

Citation

Wirths, Stefan, et al. "Activated CD8+ T Lymphocytes Induce Differentiation of Monocytes to Dendritic Cells and Restore the Stimulatory Capacity of Interleukin 10-treated Antigen-presenting Cells." Cancer Research, vol. 62, no. 17, 2002, pp. 5065-8.
Wirths S, Reichert J, Grünebach F, et al. Activated CD8+ T lymphocytes induce differentiation of monocytes to dendritic cells and restore the stimulatory capacity of interleukin 10-treated antigen-presenting cells. Cancer Res. 2002;62(17):5065-8.
Wirths, S., Reichert, J., Grünebach, F., & Brossart, P. (2002). Activated CD8+ T lymphocytes induce differentiation of monocytes to dendritic cells and restore the stimulatory capacity of interleukin 10-treated antigen-presenting cells. Cancer Research, 62(17), 5065-8.
Wirths S, et al. Activated CD8+ T Lymphocytes Induce Differentiation of Monocytes to Dendritic Cells and Restore the Stimulatory Capacity of Interleukin 10-treated Antigen-presenting Cells. Cancer Res. 2002 Sep 1;62(17):5065-8. PubMed PMID: 12208762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activated CD8+ T lymphocytes induce differentiation of monocytes to dendritic cells and restore the stimulatory capacity of interleukin 10-treated antigen-presenting cells. AU - Wirths,Stefan, AU - Reichert,Jeannette, AU - Grünebach,Frank, AU - Brossart,Peter, PY - 2002/9/5/pubmed PY - 2002/10/4/medline PY - 2002/9/5/entrez SP - 5065 EP - 8 JF - Cancer research JO - Cancer Res VL - 62 IS - 17 N2 - We analyzed the role of CD8+ T cells on dendritic cells (DCs). Incubation of monocytes with activated but not resting CD8+ T cells induced their differentiation into DCs with a CD1a+ and CD14- phenotype. Activated CD8+ lymphocytes induced down-regulation of CD14 expression in interleukin (IL)-10-treated macrophages and resulted in the expression of DC-related CD1a and up-regulation of mRNA transcripts for RelB, IL-12p40, MCP-2, MIP-1alpha, and ELC. These DCs were potent stimulatory cells in mixed lymphocyte reaction and induced primary CTL responses. This interaction was independent of a direct cell-to-cell contact. Monoclonal antibodies against IFN-gamma completely abolished the CD1a induction on IL-10-treated antigen-presenting cells. These results demonstrate that CD8+ cells can provide stimulatory signals to antigen-presenting cells to induce their differentiation in DC and to increase their immunostimulatory capacity. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/12208762/Activated_CD8+_T_lymphocytes_induce_differentiation_of_monocytes_to_dendritic_cells_and_restore_the_stimulatory_capacity_of_interleukin_10_treated_antigen_presenting_cells_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=12208762 DB - PRIME DP - Unbound Medicine ER -