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Smooth muscle expression of Cre recombinase and eGFP in transgenic mice.
Physiol Genomics. 2002 Sep 03; 10(3):211-5.PG

Abstract

We report the generation of transgenic mice designed to facilitate the study of vascular and nonvascular smooth muscle biology in vivo. The smooth muscle myosin heavy chain (smMHC) promoter was used to direct expression of a bicistronic transgene consisting of Cre recombinase and enhanced green fluorescent protein (eGFP) coding sequences. Animals expressing the transgene display strong fluorescence confined to vascular and nonvascular smooth muscle. Enzymatic dissociation of smooth muscle yields viable, fluorescent cells that can be studied as single cells or sorted by FACS for gene expression studies. smMHC/Cre/eGFP mice were crossed with ROSA26/lacZ reporter mice to determine Cre recombinase activity; Cre recombinase was expressed in all smooth muscles in adult mice, and there was an excellent overlap between expression of the recombinase and eGFP. Initial smooth muscle-specific expression of fluorescence and Cre recombinase was detected on embryonic day 12.5. These mice will be useful to define smooth muscle gene function in vivo in mice, for the study of gene function in single, live cells, and for the determination of gene expression in vascular and nonvascular smooth muscle.

Authors+Show Affiliations

Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12209023

Citation

Xin, H-B, et al. "Smooth Muscle Expression of Cre Recombinase and eGFP in Transgenic Mice." Physiological Genomics, vol. 10, no. 3, 2002, pp. 211-5.
Xin HB, Deng KY, Rishniw M, et al. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics. 2002;10(3):211-5.
Xin, H. B., Deng, K. Y., Rishniw, M., Ji, G., & Kotlikoff, M. I. (2002). Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiological Genomics, 10(3), 211-5.
Xin HB, et al. Smooth Muscle Expression of Cre Recombinase and eGFP in Transgenic Mice. Physiol Genomics. 2002 Sep 3;10(3):211-5. PubMed PMID: 12209023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. AU - Xin,H-B, AU - Deng,K-Y, AU - Rishniw,M, AU - Ji,G, AU - Kotlikoff,M I, Y1 - 2002/09/03/ PY - 2002/9/5/pubmed PY - 2002/11/26/medline PY - 2002/9/5/entrez SP - 211 EP - 5 JF - Physiological genomics JO - Physiol Genomics VL - 10 IS - 3 N2 - We report the generation of transgenic mice designed to facilitate the study of vascular and nonvascular smooth muscle biology in vivo. The smooth muscle myosin heavy chain (smMHC) promoter was used to direct expression of a bicistronic transgene consisting of Cre recombinase and enhanced green fluorescent protein (eGFP) coding sequences. Animals expressing the transgene display strong fluorescence confined to vascular and nonvascular smooth muscle. Enzymatic dissociation of smooth muscle yields viable, fluorescent cells that can be studied as single cells or sorted by FACS for gene expression studies. smMHC/Cre/eGFP mice were crossed with ROSA26/lacZ reporter mice to determine Cre recombinase activity; Cre recombinase was expressed in all smooth muscles in adult mice, and there was an excellent overlap between expression of the recombinase and eGFP. Initial smooth muscle-specific expression of fluorescence and Cre recombinase was detected on embryonic day 12.5. These mice will be useful to define smooth muscle gene function in vivo in mice, for the study of gene function in single, live cells, and for the determination of gene expression in vascular and nonvascular smooth muscle. SN - 1531-2267 UR - https://www.unboundmedicine.com/medline/citation/12209023/Smooth_muscle_expression_of_Cre_recombinase_and_eGFP_in_transgenic_mice_ L2 - https://journals.physiology.org/doi/10.1152/physiolgenomics.00054.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -