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Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus.
Arch Microbiol. 2002 Oct; 178(4):297-300.AM

Abstract

The role of Asp-328 and Ile-329 as a cofactor discrimination site of the NAD-dependent isocitrate dehydrognase (NAD-IDH) from Pyrococcus furiosus has been verified by replacing these residues with Lys and Tyr, respectively, which are the corresponding residues in NADP-IDH from Escherichia coli. The Asp-328-Lys mutant showed dual coenzyme specificity, whereas introduction of the double mutation, Asp-328-Lys/Ile-329-Tyr shifted the cofactor preference from NAD to NADP. NADP-dependent P. furiosus IDH retained thermostability and thermoactivity compared with NAD-IDH.

Authors+Show Affiliations

Department of Microbiology, University of Bergen, PO Box 7800, Jahnebakken 5, 5020 Bergen, Norway. ida.steen@im.uib.noNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12209263

Citation

Steen, Ida Helene, et al. "Identification of Cofactor Discrimination Sites in NAD-isocitrate Dehydrogenase From Pyrococcus Furiosus." Archives of Microbiology, vol. 178, no. 4, 2002, pp. 297-300.
Steen IH, Lien T, Madsen MS, et al. Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus. Arch Microbiol. 2002;178(4):297-300.
Steen, I. H., Lien, T., Madsen, M. S., & Birkeland, N. K. (2002). Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus. Archives of Microbiology, 178(4), 297-300.
Steen IH, et al. Identification of Cofactor Discrimination Sites in NAD-isocitrate Dehydrogenase From Pyrococcus Furiosus. Arch Microbiol. 2002;178(4):297-300. PubMed PMID: 12209263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus. AU - Steen,Ida Helene, AU - Lien,Torleiv, AU - Madsen,Marit Steine, AU - Birkeland,Nils-Kåre, Y1 - 2002/06/28/ PY - 2002/01/21/received PY - 2002/04/26/revised PY - 2002/04/29/accepted PY - 2002/9/5/pubmed PY - 2002/11/26/medline PY - 2002/9/5/entrez SP - 297 EP - 300 JF - Archives of microbiology JO - Arch. Microbiol. VL - 178 IS - 4 N2 - The role of Asp-328 and Ile-329 as a cofactor discrimination site of the NAD-dependent isocitrate dehydrognase (NAD-IDH) from Pyrococcus furiosus has been verified by replacing these residues with Lys and Tyr, respectively, which are the corresponding residues in NADP-IDH from Escherichia coli. The Asp-328-Lys mutant showed dual coenzyme specificity, whereas introduction of the double mutation, Asp-328-Lys/Ile-329-Tyr shifted the cofactor preference from NAD to NADP. NADP-dependent P. furiosus IDH retained thermostability and thermoactivity compared with NAD-IDH. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/12209263/Identification_of_cofactor_discrimination_sites_in_NAD_isocitrate_dehydrogenase_from_Pyrococcus_furiosus_ L2 - https://dx.doi.org/10.1007/s00203-002-0439-x DB - PRIME DP - Unbound Medicine ER -