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Coccolithovirus (Phycodnaviridae): characterisation of a new large dsDNA algal virus that infects Emiliana huxleyi.
Arch Virol. 2002 Sep; 147(9):1685-98.AV

Abstract

Emiliania huxleyi-specific viruses (EhV) were isolated from E. huxleyi blooms off the coast of Plymouth, UK, in July 1999 and July/August 2001, and from an E. huxleyi bloom induced during a mesocosm experiment in a fjord off Bergen, Norway, during June 2000. Transmission electron microscopy revealed that all 10 virus isolates are 170-200 nm in diameter with an icosahedral symmetry. Their density is approximately 1.2 in CsCl gradients and they have large double stranded DNA genomes approximately 410 kb in size. Phylogenetic analysis of the DNA polymerase genes of these viruses suggests that EhV belongs to a new genus within the family of algal viruses, Phycodnaviridae. We propose to name this new virus genus Coccolithovirus. Differences within members of the Coccolithovirus were elucidated by host range analysis of the virus isolates and sequence analysis of a gene fragment encoding part of their putative major capsid protein. All 10 virus isolates within this new genus only infected E. huxleyi strains that have previously been shown to exhibit low dimethylsulphoniopropionate lyase (DMSP-lyase) activity (CCMP1516, CCMP374 and L), while E. huxleyi strains with high DMSP-lyase activity (CCMP373 and CCMP379) were resistant to infection.

Authors+Show Affiliations

The Marine Biological Association of the United Kingdom, Plymouth, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12209309

Citation

Schroeder, D C., et al. "Coccolithovirus (Phycodnaviridae): Characterisation of a New Large dsDNA Algal Virus That Infects Emiliana Huxleyi." Archives of Virology, vol. 147, no. 9, 2002, pp. 1685-98.
Schroeder DC, Oke J, Malin G, et al. Coccolithovirus (Phycodnaviridae): characterisation of a new large dsDNA algal virus that infects Emiliana huxleyi. Arch Virol. 2002;147(9):1685-98.
Schroeder, D. C., Oke, J., Malin, G., & Wilson, W. H. (2002). Coccolithovirus (Phycodnaviridae): characterisation of a new large dsDNA algal virus that infects Emiliana huxleyi. Archives of Virology, 147(9), 1685-98.
Schroeder DC, et al. Coccolithovirus (Phycodnaviridae): Characterisation of a New Large dsDNA Algal Virus That Infects Emiliana Huxleyi. Arch Virol. 2002;147(9):1685-98. PubMed PMID: 12209309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coccolithovirus (Phycodnaviridae): characterisation of a new large dsDNA algal virus that infects Emiliana huxleyi. AU - Schroeder,D C, AU - Oke,J, AU - Malin,G, AU - Wilson,W H, PY - 2002/9/5/pubmed PY - 2002/10/11/medline PY - 2002/9/5/entrez SP - 1685 EP - 98 JF - Archives of virology JO - Arch Virol VL - 147 IS - 9 N2 - Emiliania huxleyi-specific viruses (EhV) were isolated from E. huxleyi blooms off the coast of Plymouth, UK, in July 1999 and July/August 2001, and from an E. huxleyi bloom induced during a mesocosm experiment in a fjord off Bergen, Norway, during June 2000. Transmission electron microscopy revealed that all 10 virus isolates are 170-200 nm in diameter with an icosahedral symmetry. Their density is approximately 1.2 in CsCl gradients and they have large double stranded DNA genomes approximately 410 kb in size. Phylogenetic analysis of the DNA polymerase genes of these viruses suggests that EhV belongs to a new genus within the family of algal viruses, Phycodnaviridae. We propose to name this new virus genus Coccolithovirus. Differences within members of the Coccolithovirus were elucidated by host range analysis of the virus isolates and sequence analysis of a gene fragment encoding part of their putative major capsid protein. All 10 virus isolates within this new genus only infected E. huxleyi strains that have previously been shown to exhibit low dimethylsulphoniopropionate lyase (DMSP-lyase) activity (CCMP1516, CCMP374 and L), while E. huxleyi strains with high DMSP-lyase activity (CCMP373 and CCMP379) were resistant to infection. SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/12209309/Coccolithovirus__Phycodnaviridae_:_characterisation_of_a_new_large_dsDNA_algal_virus_that_infects_Emiliana_huxleyi_ L2 - https://dx.doi.org/10.1007/s00705-002-0841-3 DB - PRIME DP - Unbound Medicine ER -