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Glial expression of estrogen and androgen receptors after rat brain injury.

Abstract

Estrogens and androgens can protect neurons from death caused by injury to the central nervous system. Astrocytes and microglia are major players in events triggered by neural lesions. To determine whether glia are direct targets of estrogens or androgens after neural insults, steroid receptor expression in glial cells was assessed in two different lesion models. An excitotoxic injury to the hippocampus or a stab wound to the parietal cortex and hippocampus was performed in male rats, and the resultant expression of steroid receptors in glial cells was assessed using double-label immunohistochemistry. Both lesions induced the expression of estrogen receptors (ERs) and androgen receptors (ARs) in glial cells. ERalpha was expressed in astrocytes immunoreactive (ERalpha-ir) for glial fibrillary acidic protein or vimentin. AR immunoreactivity colocalized with microglial markers, such as Griffonia simplicifolia lectin-1 or OX-6. The time course of ER and AR expression in glia was studied in the stab wound model. ERalpha-ir astrocytes and AR-ir microglia were observed 3 days after lesion. The number of ERalpha-ir and AR-ir glial cells reached a maximum 7 days after lesion and returned to low levels by 28 days postinjury. The studies of ERbeta expression in glia were inconclusive; different results were obtained with different antibodies. In sum, these results suggest that reactive astrocytes and reactive microglia are a direct target for estrogens and androgens, respectively.

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  • Authors+Show Affiliations

    ,

    Instituto Cajal, Consejo Superior de Investigaciones Científicas, 28002 Madrid, Spain.

    , ,

    Source

    The Journal of comparative neurology 450:3 2002 Aug 26 pg 256-71

    MeSH

    Animals
    Astrocytes
    Brain
    Brain Injuries
    Disease Models, Animal
    Estrogen Receptor alpha
    Estrogen Receptor beta
    Glial Fibrillary Acidic Protein
    Gliosis
    Immunohistochemistry
    Kainic Acid
    Lectins
    Male
    Microglia
    Nerve Degeneration
    Nerve Regeneration
    Neuroglia
    Plant Lectins
    Rats
    Rats, Wistar
    Receptors, Androgen
    Receptors, Estrogen
    Vimentin

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    12209854

    Citation

    García-Ovejero, Daniel, et al. "Glial Expression of Estrogen and Androgen Receptors After Rat Brain Injury." The Journal of Comparative Neurology, vol. 450, no. 3, 2002, pp. 256-71.
    García-Ovejero D, Veiga S, García-Segura LM, et al. Glial expression of estrogen and androgen receptors after rat brain injury. J Comp Neurol. 2002;450(3):256-71.
    García-Ovejero, D., Veiga, S., García-Segura, L. M., & Doncarlos, L. L. (2002). Glial expression of estrogen and androgen receptors after rat brain injury. The Journal of Comparative Neurology, 450(3), pp. 256-71.
    García-Ovejero D, et al. Glial Expression of Estrogen and Androgen Receptors After Rat Brain Injury. J Comp Neurol. 2002 Aug 26;450(3):256-71. PubMed PMID: 12209854.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Glial expression of estrogen and androgen receptors after rat brain injury. AU - García-Ovejero,Daniel, AU - Veiga,Sergio, AU - García-Segura,Luis Miguel, AU - Doncarlos,Lydia L, PY - 2002/9/5/pubmed PY - 2002/10/11/medline PY - 2002/9/5/entrez SP - 256 EP - 71 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 450 IS - 3 N2 - Estrogens and androgens can protect neurons from death caused by injury to the central nervous system. Astrocytes and microglia are major players in events triggered by neural lesions. To determine whether glia are direct targets of estrogens or androgens after neural insults, steroid receptor expression in glial cells was assessed in two different lesion models. An excitotoxic injury to the hippocampus or a stab wound to the parietal cortex and hippocampus was performed in male rats, and the resultant expression of steroid receptors in glial cells was assessed using double-label immunohistochemistry. Both lesions induced the expression of estrogen receptors (ERs) and androgen receptors (ARs) in glial cells. ERalpha was expressed in astrocytes immunoreactive (ERalpha-ir) for glial fibrillary acidic protein or vimentin. AR immunoreactivity colocalized with microglial markers, such as Griffonia simplicifolia lectin-1 or OX-6. The time course of ER and AR expression in glia was studied in the stab wound model. ERalpha-ir astrocytes and AR-ir microglia were observed 3 days after lesion. The number of ERalpha-ir and AR-ir glial cells reached a maximum 7 days after lesion and returned to low levels by 28 days postinjury. The studies of ERbeta expression in glia were inconclusive; different results were obtained with different antibodies. In sum, these results suggest that reactive astrocytes and reactive microglia are a direct target for estrogens and androgens, respectively. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/12209854/Glial_expression_of_estrogen_and_androgen_receptors_after_rat_brain_injury_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=2002&volume=450&issue=3&spage=256 DB - PRIME DP - Unbound Medicine ER -