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[Expression of interleukin-5 and interleukin-10 and their relationship in asthma].
Hunan Yi Ke Da Xue Xue Bao. 2000 Apr 28; 25(2):99-102.HY

Abstract

To explore the expression of interleukin-5(IL-5) and interleukin-10(IL-10) and their relationship in asthma, we measured the cells in bronchial alveolar lavage fluid(BALF) of guinea pigs, and examined the expression of IL-5 mRNA and IL-10 mRNA of bronchial tissue by reverse transcription polymerase chain reaction(RT-PCR) in control group(Group C, n = 10), asthmatic group(Group A, n = 11) and dexamethasone treatment group(Group B, n = 10). The results showed that the eosinophil(EOS) percentage and the expression of IL-5 mRNA were significantly higher in Group A[(48.64 +/- 17.8)%, (106.91 +/- 20.09)% respectively] than that in Group C[(15.10 +/- 11.48)%, (62.60 +/- 13.84)%] and Group B[(28.4 +/- 10.32)%, (75.30 +/- 10.70)%]. In contrast, the IL-10 mRNA expression in Group A[(47.5 +/- 5.3)%] was obviously lower compared with that in Group C[(101.2 +/- 4.2)%] and Group B[(80.1 +/- 8.3)%]. The correlation between the expression level of IL-5 mRNA and percentage of EOS was positive(r = 0.924, P < 0.01), whereas the IL-5 mRNA was negatively correlated with IL-10 mRNA expression(r = -0.731, P < 0.01).

CONCLUSION

IL-5 and EOS might play a role in the pathogenesis of asthma, the inhibition of IL-10 mRNA expression may cause the increase of IL-5 expression in asthma. The beneficial effects of corticosteroid treatment in asthma may partly result from increasing IL-10 mRNA expression and inhibiting IL-5 mRNA expression.

Authors+Show Affiliations

Luo BL
Department of Internal Medicine, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Wu ES
No affiliation info available
Qiu WF
No affiliation info available
Hai ZH
No affiliation info available

MeSH

AnimalsAsthmaBronchiBronchoalveolar Lavage FluidEosinophilsFemaleGuinea PigsInterleukin-10Interleukin-5MaleRNA, MessengerReverse Transcriptase Polymerase Chain Reaction

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12212231

Citation

Luo, B L., et al. "[Expression of Interleukin-5 and Interleukin-10 and Their Relationship in Asthma]." Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, vol. 25, no. 2, 2000, pp. 99-102.
Luo BL, Wu ES, Qiu WF, et al. [Expression of interleukin-5 and interleukin-10 and their relationship in asthma]. Hunan Yi Ke Da Xue Xue Bao. 2000;25(2):99-102.
Luo, B. L., Wu, E. S., Qiu, W. F., & Hai, Z. H. (2000). [Expression of interleukin-5 and interleukin-10 and their relationship in asthma]. Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, 25(2), 99-102.
Luo BL, et al. [Expression of Interleukin-5 and Interleukin-10 and Their Relationship in Asthma]. Hunan Yi Ke Da Xue Xue Bao. 2000 Apr 28;25(2):99-102. PubMed PMID: 12212231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of interleukin-5 and interleukin-10 and their relationship in asthma]. AU - Luo,B L, AU - Wu,E S, AU - Qiu,W F, AU - Hai,Z H, PY - 2002/9/6/pubmed PY - 2003/2/13/medline PY - 2002/9/6/entrez SP - 99 EP - 102 JF - Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University JO - Hunan Yi Ke Da Xue Xue Bao VL - 25 IS - 2 N2 - UNLABELLED: To explore the expression of interleukin-5(IL-5) and interleukin-10(IL-10) and their relationship in asthma, we measured the cells in bronchial alveolar lavage fluid(BALF) of guinea pigs, and examined the expression of IL-5 mRNA and IL-10 mRNA of bronchial tissue by reverse transcription polymerase chain reaction(RT-PCR) in control group(Group C, n = 10), asthmatic group(Group A, n = 11) and dexamethasone treatment group(Group B, n = 10). The results showed that the eosinophil(EOS) percentage and the expression of IL-5 mRNA were significantly higher in Group A[(48.64 +/- 17.8)%, (106.91 +/- 20.09)% respectively] than that in Group C[(15.10 +/- 11.48)%, (62.60 +/- 13.84)%] and Group B[(28.4 +/- 10.32)%, (75.30 +/- 10.70)%]. In contrast, the IL-10 mRNA expression in Group A[(47.5 +/- 5.3)%] was obviously lower compared with that in Group C[(101.2 +/- 4.2)%] and Group B[(80.1 +/- 8.3)%]. The correlation between the expression level of IL-5 mRNA and percentage of EOS was positive(r = 0.924, P < 0.01), whereas the IL-5 mRNA was negatively correlated with IL-10 mRNA expression(r = -0.731, P < 0.01). CONCLUSION: IL-5 and EOS might play a role in the pathogenesis of asthma, the inhibition of IL-10 mRNA expression may cause the increase of IL-5 expression in asthma. The beneficial effects of corticosteroid treatment in asthma may partly result from increasing IL-10 mRNA expression and inhibiting IL-5 mRNA expression. SN - 1000-5625 UR - https://www.unboundmedicine.com/medline/citation/12212231/[Expression_of_interleukin_5_and_interleukin_10_and_their_relationship_in_asthma]_ DB - PRIME DP - Unbound Medicine ER -