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Myostatin and TGF-beta2 gene expression patterns in response to in ovo administration of rhIGF-I during chicken embryonic development.
Growth Dev Aging. 2002 Summer; 66(1):3-10.GD

Abstract

The objective of the study was to evaluate the impact of in ovo administration of recombinant human insulin-like growth factor-I (rhIGF-I) on myostatin and transforming growth factor-beta2 (TGF-beta2) gene expression during chicken embryogenesis with emphasis on skeletal muscle development. Eggs were injected once with 100 ng rh IGF-I in 10 mM acetic acid, 0.1% BSA per embryo on day 3 of embryonic development. Total RNA was isolated from whole embryos on each of embryonic days (E) 0 to 6 (n = 6 per day/per treatment), from thoracic/abdominal halves of the embryo at E 7 to 8 (n = 6 per day/per treatment), and from pectoralis muscle tissues at E 9 to 20 (n = 4 per day/per treatment). Reverse-transcription polymerase chain reaction (RT-PCR) was used to synthesize cDNAs. Myostatin mRNA isolated from pectoralis muscles of the rhIGF-I treated group increased on E 10 (approximately 2.5 fold) and remained high through E 13, whereas myostatin mRNA from control pectoralis muscles increased at E 9 and remained high until E 12. TGF-beta2 gene expression from in ovo rhIGF-I treated pectoralis muscles dramatically increased at E 13 (approximately 2.5 fold), in contrast to E 14 from control pectoralis muscle, and gradually declined through E 16. Our results demonstrate that in ovo administration of rhIGF-I on E 3 may alter developmental expression patterns of myostatin and TGF-beta2 genes.

Authors+Show Affiliations

Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12212629

Citation

Kocamis, Hakan, et al. "Myostatin and TGF-beta2 Gene Expression Patterns in Response to in Ovo Administration of rhIGF-I During Chicken Embryonic Development." Growth, Development, and Aging : GDA, vol. 66, no. 1, 2002, pp. 3-10.
Kocamis H, Gahr SA, Richter J, et al. Myostatin and TGF-beta2 gene expression patterns in response to in ovo administration of rhIGF-I during chicken embryonic development. Growth Dev Aging. 2002;66(1):3-10.
Kocamis, H., Gahr, S. A., Richter, J., Kirkpatrick-Keller, D. C., & Killefer, J. (2002). Myostatin and TGF-beta2 gene expression patterns in response to in ovo administration of rhIGF-I during chicken embryonic development. Growth, Development, and Aging : GDA, 66(1), 3-10.
Kocamis H, et al. Myostatin and TGF-beta2 Gene Expression Patterns in Response to in Ovo Administration of rhIGF-I During Chicken Embryonic Development. Growth Dev Aging. 2002;66(1):3-10. PubMed PMID: 12212629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myostatin and TGF-beta2 gene expression patterns in response to in ovo administration of rhIGF-I during chicken embryonic development. AU - Kocamis,Hakan, AU - Gahr,Scott A, AU - Richter,Jennifer, AU - Kirkpatrick-Keller,Diana C, AU - Killefer,John, PY - 2002/9/6/pubmed PY - 2003/3/26/medline PY - 2002/9/6/entrez SP - 3 EP - 10 JF - Growth, development, and aging : GDA JO - Growth Dev Aging VL - 66 IS - 1 N2 - The objective of the study was to evaluate the impact of in ovo administration of recombinant human insulin-like growth factor-I (rhIGF-I) on myostatin and transforming growth factor-beta2 (TGF-beta2) gene expression during chicken embryogenesis with emphasis on skeletal muscle development. Eggs were injected once with 100 ng rh IGF-I in 10 mM acetic acid, 0.1% BSA per embryo on day 3 of embryonic development. Total RNA was isolated from whole embryos on each of embryonic days (E) 0 to 6 (n = 6 per day/per treatment), from thoracic/abdominal halves of the embryo at E 7 to 8 (n = 6 per day/per treatment), and from pectoralis muscle tissues at E 9 to 20 (n = 4 per day/per treatment). Reverse-transcription polymerase chain reaction (RT-PCR) was used to synthesize cDNAs. Myostatin mRNA isolated from pectoralis muscles of the rhIGF-I treated group increased on E 10 (approximately 2.5 fold) and remained high through E 13, whereas myostatin mRNA from control pectoralis muscles increased at E 9 and remained high until E 12. TGF-beta2 gene expression from in ovo rhIGF-I treated pectoralis muscles dramatically increased at E 13 (approximately 2.5 fold), in contrast to E 14 from control pectoralis muscle, and gradually declined through E 16. Our results demonstrate that in ovo administration of rhIGF-I on E 3 may alter developmental expression patterns of myostatin and TGF-beta2 genes. SN - 1041-1232 UR - https://www.unboundmedicine.com/medline/citation/12212629/Myostatin_and_TGF_beta2_gene_expression_patterns_in_response_to_in_ovo_administration_of_rhIGF_I_during_chicken_embryonic_development_ DB - PRIME DP - Unbound Medicine ER -