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cDNA cloning of a salivary chymotrypsin-like protease and the identification of six additional cDNAs encoding putative digestive proteases from the green mirid, Creontiades dilutus (Hemiptera: Miridae).
Insect Biochem Mol Biol. 2002 Sep; 32(9):1065-75.IB

Abstract

RT-PCR with degenerate primers was used to amplify partial cDNA fragments for one serine protease gene and three cysteine protease genes from poly(A) RNA isolated from the midgut of the green mirid, Creontiades dilutus. The serine protease amplicon showed homology to insect trypsin-like protease genes, and all three cysteine protease amplicons showed homology to cathepsin L-like protease genes.RT-PCR was also used to amplify fragments of three serine protease genes from salivary gland poly(A) RNA. One of these salivary gland serine protease amplicons was used to screen a whole organism cDNA library to isolate a full length cDNA clone, designated CdSp1 (Accession AY055753), which encodes a putative chymotrypsin-like protease. CdSp1 codes for a 293 amino acid protein that contains a signal peptide and activation peptide, as well as the catalytic triad present in all serine proteases and several of the binding pocket residues characteristic of chymotrypsins. In situ hybridisation showed that the transcript is expressed in the posterior lobe of the principal salivary gland, but not in the anterior lobe of the principal salivary gland, the accessory salivary gland or the midgut.

Authors+Show Affiliations

Division of Entomology, CSIRO, Canberra, ACT, Australia. gillian.colebatch@csiro.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12213243

Citation

Colebatch, G, et al. "CDNA Cloning of a Salivary Chymotrypsin-like Protease and the Identification of Six Additional cDNAs Encoding Putative Digestive Proteases From the Green Mirid, Creontiades Dilutus (Hemiptera: Miridae)." Insect Biochemistry and Molecular Biology, vol. 32, no. 9, 2002, pp. 1065-75.
Colebatch G, Cooper P, East P. CDNA cloning of a salivary chymotrypsin-like protease and the identification of six additional cDNAs encoding putative digestive proteases from the green mirid, Creontiades dilutus (Hemiptera: Miridae). Insect Biochem Mol Biol. 2002;32(9):1065-75.
Colebatch, G., Cooper, P., & East, P. (2002). CDNA cloning of a salivary chymotrypsin-like protease and the identification of six additional cDNAs encoding putative digestive proteases from the green mirid, Creontiades dilutus (Hemiptera: Miridae). Insect Biochemistry and Molecular Biology, 32(9), 1065-75.
Colebatch G, Cooper P, East P. CDNA Cloning of a Salivary Chymotrypsin-like Protease and the Identification of Six Additional cDNAs Encoding Putative Digestive Proteases From the Green Mirid, Creontiades Dilutus (Hemiptera: Miridae). Insect Biochem Mol Biol. 2002;32(9):1065-75. PubMed PMID: 12213243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - cDNA cloning of a salivary chymotrypsin-like protease and the identification of six additional cDNAs encoding putative digestive proteases from the green mirid, Creontiades dilutus (Hemiptera: Miridae). AU - Colebatch,G, AU - Cooper,P, AU - East,P, PY - 2002/9/6/pubmed PY - 2002/11/26/medline PY - 2002/9/6/entrez SP - 1065 EP - 75 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 32 IS - 9 N2 - RT-PCR with degenerate primers was used to amplify partial cDNA fragments for one serine protease gene and three cysteine protease genes from poly(A) RNA isolated from the midgut of the green mirid, Creontiades dilutus. The serine protease amplicon showed homology to insect trypsin-like protease genes, and all three cysteine protease amplicons showed homology to cathepsin L-like protease genes.RT-PCR was also used to amplify fragments of three serine protease genes from salivary gland poly(A) RNA. One of these salivary gland serine protease amplicons was used to screen a whole organism cDNA library to isolate a full length cDNA clone, designated CdSp1 (Accession AY055753), which encodes a putative chymotrypsin-like protease. CdSp1 codes for a 293 amino acid protein that contains a signal peptide and activation peptide, as well as the catalytic triad present in all serine proteases and several of the binding pocket residues characteristic of chymotrypsins. In situ hybridisation showed that the transcript is expressed in the posterior lobe of the principal salivary gland, but not in the anterior lobe of the principal salivary gland, the accessory salivary gland or the midgut. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/12213243/cDNA_cloning_of_a_salivary_chymotrypsin_like_protease_and_the_identification_of_six_additional_cDNAs_encoding_putative_digestive_proteases_from_the_green_mirid_Creontiades_dilutus__Hemiptera:_Miridae__ DB - PRIME DP - Unbound Medicine ER -