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The U1 snRNP protein U1C recognizes the 5' splice site in the absence of base pairing.
Nature. 2002 Sep 05; 419(6902):86-90.Nat

Abstract

Splicing of precursor messenger RNA takes place in the spliceosome, a large RNA/protein macromolecular machine. Spliceosome assembly occurs in an ordered pathway in vitro and is conserved between yeast and mammalian systems. The earliest step is commitment complex formation in yeast or E complex formation in mammals, which engages the pre-mRNA in the splicing pathway and involves interactions between U1 small nuclear ribonucleoprotein (snRNP) and the pre-mRNA 5' splice site. Complex formation depends on highly conserved base pairing between the 5' splice site and the 5' end of U1 snRNA, both in vivo and in vitro. U1 snRNP proteins also contribute to U1 snRNP activity. Here we show that U1 snRNP lacking the 5' end of its snRNA retains 5'-splice-site sequence specificity. We also show that recombinant yeast U1C protein, a U1 snRNP protein, selects a 5'-splice-site-like sequence in which the first four nucleotides, GUAU, are identical to the first four nucleotides of the yeast 5'-splice-site consensus sequence. We propose that a U1C 5'-splice-site interaction precedes pre-mRNA/U1 snRNA base pairing and is the earliest step in the splicing pathway.

Authors+Show Affiliations

Howard Hughes Medical Institute, Department of Biology, MS008, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12214237

Citation

Du, Hansen, and Michael Rosbash. "The U1 snRNP Protein U1C Recognizes the 5' Splice Site in the Absence of Base Pairing." Nature, vol. 419, no. 6902, 2002, pp. 86-90.
Du H, Rosbash M. The U1 snRNP protein U1C recognizes the 5' splice site in the absence of base pairing. Nature. 2002;419(6902):86-90.
Du, H., & Rosbash, M. (2002). The U1 snRNP protein U1C recognizes the 5' splice site in the absence of base pairing. Nature, 419(6902), 86-90.
Du H, Rosbash M. The U1 snRNP Protein U1C Recognizes the 5' Splice Site in the Absence of Base Pairing. Nature. 2002 Sep 5;419(6902):86-90. PubMed PMID: 12214237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The U1 snRNP protein U1C recognizes the 5' splice site in the absence of base pairing. AU - Du,Hansen, AU - Rosbash,Michael, PY - 2002/9/6/pubmed PY - 2002/10/9/medline PY - 2002/9/6/entrez SP - 86 EP - 90 JF - Nature JO - Nature VL - 419 IS - 6902 N2 - Splicing of precursor messenger RNA takes place in the spliceosome, a large RNA/protein macromolecular machine. Spliceosome assembly occurs in an ordered pathway in vitro and is conserved between yeast and mammalian systems. The earliest step is commitment complex formation in yeast or E complex formation in mammals, which engages the pre-mRNA in the splicing pathway and involves interactions between U1 small nuclear ribonucleoprotein (snRNP) and the pre-mRNA 5' splice site. Complex formation depends on highly conserved base pairing between the 5' splice site and the 5' end of U1 snRNA, both in vivo and in vitro. U1 snRNP proteins also contribute to U1 snRNP activity. Here we show that U1 snRNP lacking the 5' end of its snRNA retains 5'-splice-site sequence specificity. We also show that recombinant yeast U1C protein, a U1 snRNP protein, selects a 5'-splice-site-like sequence in which the first four nucleotides, GUAU, are identical to the first four nucleotides of the yeast 5'-splice-site consensus sequence. We propose that a U1C 5'-splice-site interaction precedes pre-mRNA/U1 snRNA base pairing and is the earliest step in the splicing pathway. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/12214237/The_U1_snRNP_protein_U1C_recognizes_the_5'_splice_site_in_the_absence_of_base_pairing_ L2 - https://doi.org/10.1038/nature00947 DB - PRIME DP - Unbound Medicine ER -