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Keratinocyte growth factor stimulates the migration and proliferation of breast cancer cells in a culture wounding model.
Pharmacol Res. 2002 Aug; 46(2):179-83.PR

Abstract

Growth factors are known to influence the progression, motility and invasiveness of tumor cells. In a previous study, we reported that conditioned media from NIH 3T3 cells (mouse fibroblast), which contains KGF, increased the motile morphology of estrogen receptor (ER)-positive breast cancer cells and produced no effect on ER-negative cells. The present study examined the influence of human KGF on two estrogen receptor (ER)-positive human breast cancer cell lines (MCF-7 and T-47D) using a culture wounding model to evaluate cell proliferation and migration over a period of 48h. In the present study we observed that KGF enhanced the migration and proliferation of both MCF-7 and T-47D breast cancer cells. In both cell lines the response to KGF was found to be both dose- and time-dependent. However, the total migration and proliferation response of the MCF-7 cells to KGF was much greater than that observed in the T-47D cells. The results of this study demonstrate that human KGF enhances the migration and proliferation of human breast cancer cells. Further, these results support the concept that KGF may be an early signal in the progression of breast cancer to a more motile and metastatic phenotype.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, College of Pharmacy, Health Sciences Center, University of Oklahoma, 1110 N. Stonewall, Oklahoma City, OK 73117, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12220958

Citation

Nguyen, Thao-Nguyen, et al. "Keratinocyte Growth Factor Stimulates the Migration and Proliferation of Breast Cancer Cells in a Culture Wounding Model." Pharmacological Research, vol. 46, no. 2, 2002, pp. 179-83.
Nguyen TN, Zang XP, Pento JT. Keratinocyte growth factor stimulates the migration and proliferation of breast cancer cells in a culture wounding model. Pharmacol Res. 2002;46(2):179-83.
Nguyen, T. N., Zang, X. P., & Pento, J. T. (2002). Keratinocyte growth factor stimulates the migration and proliferation of breast cancer cells in a culture wounding model. Pharmacological Research, 46(2), 179-83.
Nguyen TN, Zang XP, Pento JT. Keratinocyte Growth Factor Stimulates the Migration and Proliferation of Breast Cancer Cells in a Culture Wounding Model. Pharmacol Res. 2002;46(2):179-83. PubMed PMID: 12220958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Keratinocyte growth factor stimulates the migration and proliferation of breast cancer cells in a culture wounding model. AU - Nguyen,Thao-Nguyen, AU - Zang,Xiao-Ping, AU - Pento,J Thomas, PY - 2002/9/11/pubmed PY - 2003/4/11/medline PY - 2002/9/11/entrez SP - 179 EP - 83 JF - Pharmacological research JO - Pharmacol Res VL - 46 IS - 2 N2 - Growth factors are known to influence the progression, motility and invasiveness of tumor cells. In a previous study, we reported that conditioned media from NIH 3T3 cells (mouse fibroblast), which contains KGF, increased the motile morphology of estrogen receptor (ER)-positive breast cancer cells and produced no effect on ER-negative cells. The present study examined the influence of human KGF on two estrogen receptor (ER)-positive human breast cancer cell lines (MCF-7 and T-47D) using a culture wounding model to evaluate cell proliferation and migration over a period of 48h. In the present study we observed that KGF enhanced the migration and proliferation of both MCF-7 and T-47D breast cancer cells. In both cell lines the response to KGF was found to be both dose- and time-dependent. However, the total migration and proliferation response of the MCF-7 cells to KGF was much greater than that observed in the T-47D cells. The results of this study demonstrate that human KGF enhances the migration and proliferation of human breast cancer cells. Further, these results support the concept that KGF may be an early signal in the progression of breast cancer to a more motile and metastatic phenotype. SN - 1043-6618 UR - https://www.unboundmedicine.com/medline/citation/12220958/Keratinocyte_growth_factor_stimulates_the_migration_and_proliferation_of_breast_cancer_cells_in_a_culture_wounding_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043661802000853 DB - PRIME DP - Unbound Medicine ER -