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A photoactivatable GFP for selective photolabeling of proteins and cells.
Science. 2002 Sep 13; 297(5588):1873-7.Sci

Abstract

We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange.

Authors+Show Affiliations

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12228718

Citation

Patterson, George H., and Jennifer Lippincott-Schwartz. "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells." Science (New York, N.Y.), vol. 297, no. 5588, 2002, pp. 1873-7.
Patterson GH, Lippincott-Schwartz J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science. 2002;297(5588):1873-7.
Patterson, G. H., & Lippincott-Schwartz, J. (2002). A photoactivatable GFP for selective photolabeling of proteins and cells. Science (New York, N.Y.), 297(5588), 1873-7.
Patterson GH, Lippincott-Schwartz J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science. 2002 Sep 13;297(5588):1873-7. PubMed PMID: 12228718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A photoactivatable GFP for selective photolabeling of proteins and cells. AU - Patterson,George H, AU - Lippincott-Schwartz,Jennifer, PY - 2002/9/14/pubmed PY - 2002/10/9/medline PY - 2002/9/14/entrez SP - 1873 EP - 7 JF - Science (New York, N.Y.) JO - Science VL - 297 IS - 5588 N2 - We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/12228718/A_photoactivatable_GFP_for_selective_photolabeling_of_proteins_and_cells_ L2 - http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=12228718 DB - PRIME DP - Unbound Medicine ER -