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The CAG trinucleotide repeat length in the androgen receptor does not predict the early onset of prostate cancer.
BJU Int. 2002 Oct; 90(6):573-8.BI

Abstract

OBJECTIVE

To relate the repeat length of the androgen-receptor CAG trinucleotide to the age of onset of prostate cancer, stage and grade of disease.

PATIENTS AND METHODS

After obtaining ethical approval, 265 patients with locally confined or locally advanced/metastatic prostate cancer were identified and evaluated for age at diagnosis (< 65 years and > 75 years). DNA was extracted from peripheral blood lymphocytes and 1 micro g aliquots subjected to polymerase chain reaction using fluorescently labelled primers. Samples were then run on an ABI 377 gene scan analysis gel with an internal molecular weight marker. The length of the CAG repeat was determined by comparing the gene scan product size to samples where the CAG repeat length had been quantified using direct sequencing. The Kruskal-Wallis, Mann-Whitney and Wilcoxon two sample tests were used to analyse the data.

RESULTS

The mean (range) length of the CAG repeat in the androgen receptor was 22.2 (10-31) in the younger and 22.5 (16-32) in the older group, and was not statistically different. There was no significant association between the CAG repeat length and the age of onset of prostate cancer (P = 0.568) or with stage (P = 0.577) and grade (P = 0.891) of prostate cancer.

CONCLUSION

These results suggest that there is no correlation between the androgen receptor CAG repeat length and the age of onset, stage and grade of prostate cancer, confirming recent doubts from other similar studies of a suggested correlation between shorter androgen receptor CAG repeat and early onset and aggressiveness of prostate cancer.

Authors+Show Affiliations

Department of Surgery, Glasgow Royal Infirmary, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12230620

Citation

Mir, K, et al. "The CAG Trinucleotide Repeat Length in the Androgen Receptor Does Not Predict the Early Onset of Prostate Cancer." BJU International, vol. 90, no. 6, 2002, pp. 573-8.
Mir K, Edwards J, Paterson PJ, et al. The CAG trinucleotide repeat length in the androgen receptor does not predict the early onset of prostate cancer. BJU Int. 2002;90(6):573-8.
Mir, K., Edwards, J., Paterson, P. J., Hehir, M., Underwood, M. A., & Bartlett, J. M. (2002). The CAG trinucleotide repeat length in the androgen receptor does not predict the early onset of prostate cancer. BJU International, 90(6), 573-8.
Mir K, et al. The CAG Trinucleotide Repeat Length in the Androgen Receptor Does Not Predict the Early Onset of Prostate Cancer. BJU Int. 2002;90(6):573-8. PubMed PMID: 12230620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The CAG trinucleotide repeat length in the androgen receptor does not predict the early onset of prostate cancer. AU - Mir,K, AU - Edwards,J, AU - Paterson,P J, AU - Hehir,M, AU - Underwood,M A, AU - Bartlett,J M S, PY - 2002/9/17/pubmed PY - 2002/10/31/medline PY - 2002/9/17/entrez SP - 573 EP - 8 JF - BJU international JO - BJU Int. VL - 90 IS - 6 N2 - OBJECTIVE: To relate the repeat length of the androgen-receptor CAG trinucleotide to the age of onset of prostate cancer, stage and grade of disease. PATIENTS AND METHODS: After obtaining ethical approval, 265 patients with locally confined or locally advanced/metastatic prostate cancer were identified and evaluated for age at diagnosis (< 65 years and > 75 years). DNA was extracted from peripheral blood lymphocytes and 1 micro g aliquots subjected to polymerase chain reaction using fluorescently labelled primers. Samples were then run on an ABI 377 gene scan analysis gel with an internal molecular weight marker. The length of the CAG repeat was determined by comparing the gene scan product size to samples where the CAG repeat length had been quantified using direct sequencing. The Kruskal-Wallis, Mann-Whitney and Wilcoxon two sample tests were used to analyse the data. RESULTS: The mean (range) length of the CAG repeat in the androgen receptor was 22.2 (10-31) in the younger and 22.5 (16-32) in the older group, and was not statistically different. There was no significant association between the CAG repeat length and the age of onset of prostate cancer (P = 0.568) or with stage (P = 0.577) and grade (P = 0.891) of prostate cancer. CONCLUSION: These results suggest that there is no correlation between the androgen receptor CAG repeat length and the age of onset, stage and grade of prostate cancer, confirming recent doubts from other similar studies of a suggested correlation between shorter androgen receptor CAG repeat and early onset and aggressiveness of prostate cancer. SN - 1464-4096 UR - https://www.unboundmedicine.com/medline/citation/12230620/The_CAG_trinucleotide_repeat_length_in_the_androgen_receptor_does_not_predict_the_early_onset_of_prostate_cancer_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=1464-4096&amp;date=2002&amp;volume=90&amp;issue=6&amp;spage=573 DB - PRIME DP - Unbound Medicine ER -