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Absence of endogenous interleukin-10 enhances the evolution of acute lung injury.
Eur Cytokine Netw. 2002 Jul-Sep; 13(3):285-97.EC

Abstract

Interleukin-10 (IL-10) exerts a wide spectrum of regulatory activities in the immune and inflammatory response. The aim of this study was to investigate the role of endogenous IL-10 on the modulation of the inflammatory response in mice subjected to carrageenan-induced lung injury. When compared to carrageenan-treated IL-10 wild-type (WT) mice, carrageenan-treated IL-10 knock-out mice (IL-10KO) mice experienced a higher rate of pleural exudation, and polymorphonuclear cell migration. Exudate levels of the pro-inflammatory cytokines tumour necrosis factor, interleukin-1beta and interleukin-6 were also greatly enhanced in IL-10KO mice in comparison to wild-type mice. Lung myeloperoxidase (MPO) activity was significantly reduced in IL-10WT mice when compared to IL-10KO mice-treated with carrageenan. The degree of oxidative and nitrosative damage was significantly higher in IL-10KO mice than in wild-type littermates, as indicated by elevated malondialdehyde levels and formation of nitrotyrosine and poly (ADP-ribose) synthetase (PARS). Staining of lung tissue sections obtained from carrageenan-treated IL-10WT with an anti-COX-2 antibody showed a positive staining of the inflamed tissue. Furthermore, expression of inducible nitric oxide synthase (iNOS) was found mainly in the macrophages of the inflamed lungs from carrageenan-treated IL-10WT mice. The intensity and degree of the staining for COX-2 and iNOS were markedly enhanced in tissue sections obtained from carrageenan-treated IL-10KO mice. Most notably, the degree of lung injury caused by carrageenan was also enhanced in IL-10KO mice. Taken together, our results clearly demonstrate that endogenous IL-10 exerts an anti-inflammatory role during acute inflammation and tissue damage associated with carrageenan-induced pleurisy, possibly by regulating neutrophil recruitment, and the subsequent cytokine and oxidant generation.

Authors+Show Affiliations

Institute of Pharmacology, School of Medicine, University of Messina, Torre Biologica--Policlinico Universitario Via C. Valeria, Gazzi, 98100 Messina Italy. salvatore@www.unime.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12231472

Citation

Cuzzocrea, Salvatore, et al. "Absence of Endogenous Interleukin-10 Enhances the Evolution of Acute Lung Injury." European Cytokine Network, vol. 13, no. 3, 2002, pp. 285-97.
Cuzzocrea S, Mazzon E, Dugo L, et al. Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. Eur Cytokine Netw. 2002;13(3):285-97.
Cuzzocrea, S., Mazzon, E., Dugo, L., Serraino, I., Di Paola, R., Genovese, T., De Sarro, A., & Caputi, A. P. (2002). Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. European Cytokine Network, 13(3), 285-97.
Cuzzocrea S, et al. Absence of Endogenous Interleukin-10 Enhances the Evolution of Acute Lung Injury. Eur Cytokine Netw. 2002 Jul-Sep;13(3):285-97. PubMed PMID: 12231472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. AU - Cuzzocrea,Salvatore, AU - Mazzon,Emanuela, AU - Dugo,Laura, AU - Serraino,Ivana, AU - Di Paola,Rosanna, AU - Genovese,Tiziana, AU - De Sarro,Angela, AU - Caputi,Achille P, PY - 2002/9/17/pubmed PY - 2003/3/5/medline PY - 2002/9/17/entrez SP - 285 EP - 97 JF - European cytokine network JO - Eur Cytokine Netw VL - 13 IS - 3 N2 - Interleukin-10 (IL-10) exerts a wide spectrum of regulatory activities in the immune and inflammatory response. The aim of this study was to investigate the role of endogenous IL-10 on the modulation of the inflammatory response in mice subjected to carrageenan-induced lung injury. When compared to carrageenan-treated IL-10 wild-type (WT) mice, carrageenan-treated IL-10 knock-out mice (IL-10KO) mice experienced a higher rate of pleural exudation, and polymorphonuclear cell migration. Exudate levels of the pro-inflammatory cytokines tumour necrosis factor, interleukin-1beta and interleukin-6 were also greatly enhanced in IL-10KO mice in comparison to wild-type mice. Lung myeloperoxidase (MPO) activity was significantly reduced in IL-10WT mice when compared to IL-10KO mice-treated with carrageenan. The degree of oxidative and nitrosative damage was significantly higher in IL-10KO mice than in wild-type littermates, as indicated by elevated malondialdehyde levels and formation of nitrotyrosine and poly (ADP-ribose) synthetase (PARS). Staining of lung tissue sections obtained from carrageenan-treated IL-10WT with an anti-COX-2 antibody showed a positive staining of the inflamed tissue. Furthermore, expression of inducible nitric oxide synthase (iNOS) was found mainly in the macrophages of the inflamed lungs from carrageenan-treated IL-10WT mice. The intensity and degree of the staining for COX-2 and iNOS were markedly enhanced in tissue sections obtained from carrageenan-treated IL-10KO mice. Most notably, the degree of lung injury caused by carrageenan was also enhanced in IL-10KO mice. Taken together, our results clearly demonstrate that endogenous IL-10 exerts an anti-inflammatory role during acute inflammation and tissue damage associated with carrageenan-induced pleurisy, possibly by regulating neutrophil recruitment, and the subsequent cytokine and oxidant generation. SN - 1148-5493 UR - https://www.unboundmedicine.com/medline/citation/12231472/Absence_of_endogenous_interleukin_10_enhances_the_evolution_of_acute_lung_injury_ L2 - http://www.jle.com/medline.md?issn=1148-5493&vol=13&iss=3&page=285 DB - PRIME DP - Unbound Medicine ER -