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Regulation of Erwinia carotovora hrpL(Ecc) (sigma-L(Ecc)), which encodes an extracytoplasmic function subfamily of sigma factor required for expression of the HRP regulon.
Mol Plant Microbe Interact. 2002 Sep; 15(9):971-80.MP

Abstract

In Erwinia carotovora subsp. carotovora (Ecc) strain 71 (Ecc71), HrpL(Ecc), an alternate sigma factor of the extracytoplasmic function subfamily, plays a central role in the expression of the hrp (hypersensitive reaction and pathogenicity) regulon. We document here that sigma-54 (RpoN) is required for full expression of hrpL(Ecc) and that HrpS, in conjunction with sigma-54, activates hrpL(Ecc) transcription. We also made the novel observation that integration host factor is required for the activation of the hrpL(Ecc) promoter. Our findings reveal that the RsmA/rsmB RNA-mediated post-transcriptional system, known to control extracellular enzyme and harpin production, affects hrpL(Ecc) expression as well. For example, hrpL(Ecc) RNA levels are barely detected in an RsmB- strain. Conversely, hrpL(Ecc) mRNA levels are much higher in RsmA- bacteria than in the RsmA+ parent. This effect is due to RsmA-promoted decay of hrpL(Ecc) RNA. Moreover, the following regulators known to control the production of either RsmA, rsmB RNA, or both also affect hrpL(Ecc) expression: GacA (response regulator of a two-component system), KdgR (an IcII type repressor), HexA (a LysR type repressor), RsmC (a putative transcriptional adapter). Based upon the data now available for Ecc and extrapolating from the evidence in other systems, we propose a tentative model that depicts the Hrp regulatory system of Ecc and explains the basis for coregulation of extracellular enzyme production and expression of the Hrp regulon.

Authors+Show Affiliations

Department of Plant Microbiology and Pathology, University of Missouri at Columbia, 65211, USA. chatterjeeas@missouri.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12236604

Citation

Chatterjee, Asita, et al. "Regulation of Erwinia Carotovora hrpL(Ecc) (sigma-L(Ecc)), Which Encodes an Extracytoplasmic Function Subfamily of Sigma Factor Required for Expression of the HRP Regulon." Molecular Plant-microbe Interactions : MPMI, vol. 15, no. 9, 2002, pp. 971-80.
Chatterjee A, Cui Y, Chatterjee AK. Regulation of Erwinia carotovora hrpL(Ecc) (sigma-L(Ecc)), which encodes an extracytoplasmic function subfamily of sigma factor required for expression of the HRP regulon. Mol Plant Microbe Interact. 2002;15(9):971-80.
Chatterjee, A., Cui, Y., & Chatterjee, A. K. (2002). Regulation of Erwinia carotovora hrpL(Ecc) (sigma-L(Ecc)), which encodes an extracytoplasmic function subfamily of sigma factor required for expression of the HRP regulon. Molecular Plant-microbe Interactions : MPMI, 15(9), 971-80.
Chatterjee A, Cui Y, Chatterjee AK. Regulation of Erwinia Carotovora hrpL(Ecc) (sigma-L(Ecc)), Which Encodes an Extracytoplasmic Function Subfamily of Sigma Factor Required for Expression of the HRP Regulon. Mol Plant Microbe Interact. 2002;15(9):971-80. PubMed PMID: 12236604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of Erwinia carotovora hrpL(Ecc) (sigma-L(Ecc)), which encodes an extracytoplasmic function subfamily of sigma factor required for expression of the HRP regulon. AU - Chatterjee,Asita, AU - Cui,Yaya, AU - Chatterjee,Arun K, PY - 2002/9/19/pubmed PY - 2003/2/21/medline PY - 2002/9/19/entrez SP - 971 EP - 80 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 15 IS - 9 N2 - In Erwinia carotovora subsp. carotovora (Ecc) strain 71 (Ecc71), HrpL(Ecc), an alternate sigma factor of the extracytoplasmic function subfamily, plays a central role in the expression of the hrp (hypersensitive reaction and pathogenicity) regulon. We document here that sigma-54 (RpoN) is required for full expression of hrpL(Ecc) and that HrpS, in conjunction with sigma-54, activates hrpL(Ecc) transcription. We also made the novel observation that integration host factor is required for the activation of the hrpL(Ecc) promoter. Our findings reveal that the RsmA/rsmB RNA-mediated post-transcriptional system, known to control extracellular enzyme and harpin production, affects hrpL(Ecc) expression as well. For example, hrpL(Ecc) RNA levels are barely detected in an RsmB- strain. Conversely, hrpL(Ecc) mRNA levels are much higher in RsmA- bacteria than in the RsmA+ parent. This effect is due to RsmA-promoted decay of hrpL(Ecc) RNA. Moreover, the following regulators known to control the production of either RsmA, rsmB RNA, or both also affect hrpL(Ecc) expression: GacA (response regulator of a two-component system), KdgR (an IcII type repressor), HexA (a LysR type repressor), RsmC (a putative transcriptional adapter). Based upon the data now available for Ecc and extrapolating from the evidence in other systems, we propose a tentative model that depicts the Hrp regulatory system of Ecc and explains the basis for coregulation of extracellular enzyme production and expression of the Hrp regulon. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/12236604/Regulation_of_Erwinia_carotovora_hrpL_Ecc___sigma_L_Ecc___which_encodes_an_extracytoplasmic_function_subfamily_of_sigma_factor_required_for_expression_of_the_HRP_regulon_ L2 - https://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2002.15.9.971?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -