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Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions.
J Neurosci Res. 2002 Oct 01; 70(1):108-16.JN

Abstract

Despite a wealth of data on the neurotoxic effects of lead at the cellular and molecular levels, the reasons for its development-dependent neurotoxicity are still unclear. Here, the maturation-dependent effects of lead acetate were analyzed in immature and differentiated brain cells cultured in aggregates. Markers of general cytotoxicity as well as cell-type-specific markers of glial and neuronal cells showed that immature brain cells were more sensitive to lead than the differentiated counterparts, demonstrating that the development-dependent neurotoxicity of lead can be reproduced in aggregating brain cell cultures. After 10 days of treatment, astrocytes were found to be more affected by lead acetate than neurons in immature cultures, and microglial cells were strongly activated. Eleven days after cessation of the treatment, lead acetate caused a partial loss of astrocytes and an intense reactivity of the remaining ones. Furthermore, microglial cells expressed a macrophagic phenotype, and the loss of activity of neuron-specific enzymes was aggravated. In differentiated cultures, no reactive gliosis was found. It is hypothetized that the intense glial reactions (microgliosis and astrogliosis) observed in immature cultures contribute to the development-dependent neurotoxicity of lead.

Authors+Show Affiliations

Institute of Physiology, University of Lausanne, Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12237869

Citation

Zurich, Marie-Gabrielle, et al. "Maturation-dependent Neurotoxicity of Lead Acetate in Vitro: Implication of Glial Reactions." Journal of Neuroscience Research, vol. 70, no. 1, 2002, pp. 108-16.
Zurich MG, Eskes C, Honegger P, et al. Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions. J Neurosci Res. 2002;70(1):108-16.
Zurich, M. G., Eskes, C., Honegger, P., Bérode, M., & Monnet-Tschudi, F. (2002). Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions. Journal of Neuroscience Research, 70(1), 108-16.
Zurich MG, et al. Maturation-dependent Neurotoxicity of Lead Acetate in Vitro: Implication of Glial Reactions. J Neurosci Res. 2002 Oct 1;70(1):108-16. PubMed PMID: 12237869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions. AU - Zurich,Marie-Gabrielle, AU - Eskes,Chantra, AU - Honegger,Paul, AU - Bérode,Michèle, AU - Monnet-Tschudi,Florianne, PY - 2002/9/19/pubmed PY - 2002/11/26/medline PY - 2002/9/19/entrez SP - 108 EP - 16 JF - Journal of neuroscience research JO - J Neurosci Res VL - 70 IS - 1 N2 - Despite a wealth of data on the neurotoxic effects of lead at the cellular and molecular levels, the reasons for its development-dependent neurotoxicity are still unclear. Here, the maturation-dependent effects of lead acetate were analyzed in immature and differentiated brain cells cultured in aggregates. Markers of general cytotoxicity as well as cell-type-specific markers of glial and neuronal cells showed that immature brain cells were more sensitive to lead than the differentiated counterparts, demonstrating that the development-dependent neurotoxicity of lead can be reproduced in aggregating brain cell cultures. After 10 days of treatment, astrocytes were found to be more affected by lead acetate than neurons in immature cultures, and microglial cells were strongly activated. Eleven days after cessation of the treatment, lead acetate caused a partial loss of astrocytes and an intense reactivity of the remaining ones. Furthermore, microglial cells expressed a macrophagic phenotype, and the loss of activity of neuron-specific enzymes was aggravated. In differentiated cultures, no reactive gliosis was found. It is hypothetized that the intense glial reactions (microgliosis and astrogliosis) observed in immature cultures contribute to the development-dependent neurotoxicity of lead. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/12237869/Maturation_dependent_neurotoxicity_of_lead_acetate_in_vitro:_implication_of_glial_reactions_ DB - PRIME DP - Unbound Medicine ER -