Tags

Type your tag names separated by a space and hit enter

Stimulation of the murine type II transforming growth factor-beta receptor promoter by the transcription factor Egr-1.
Mol Reprod Dev. 2002 Nov; 63(3):282-90.MR

Abstract

Previous studies have demonstrated that differentiation of murine embryonal carcinoma (EC) cells leads to the appearance of high affinity receptors for transforming growth factor-beta (TGF-beta). Subsequently, it was demonstrated that differentiation of F9 EC cells leads to increases in the transcription of the type II TGF-beta-receptor gene (TbetaR-II) and leads to significant increases in the steady-state levels of TbetaR-II mRNA. Analysis of the human TbetaR-II promoter in F9-differentiated cells identified several cis-regulatory elements that influence the activity of the promoter, including a CRE/ATF site and a CCAAT box motif. In the work described in this report, we focused on the effect of the transcription factor Egr-1 on the murine TbetaR-II promoter. We have identified an Egr-1 response-element approximately 150 bp upstream of the major transcription start site of the murine TbetaR-II gene. We demonstrate by electrophoretic mobility shift analysis (EMSA) that this cis-regulatory element binds Egr-1, and we demonstrate that disruption of this site eliminates the response to Egr-1. As part of this analysis, we also examined the effect of Egr-1 on human TbetaR-II promoter. In contrast to a previous report, which reported that Egr-1 inhibits expression of human TbetaR-II promoter/reporter gene constructs, we did not observe an inhibitory effect of Egr-1 that was specific for the human TbetaR-II promoter. Taken together, the findings described in this report identify important differences between the human and the murine TbetaR-II promoter, and our findings identify an Egr-1 cis-regulatory element that is capable of stimulating the activity of the murine TbetaR-II promoter.

Authors+Show Affiliations

Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12237943

Citation

Wilder, Phillip J., et al. "Stimulation of the Murine Type II Transforming Growth Factor-beta Receptor Promoter By the Transcription Factor Egr-1." Molecular Reproduction and Development, vol. 63, no. 3, 2002, pp. 282-90.
Wilder PJ, Bernadt CT, Kim JH, et al. Stimulation of the murine type II transforming growth factor-beta receptor promoter by the transcription factor Egr-1. Mol Reprod Dev. 2002;63(3):282-90.
Wilder, P. J., Bernadt, C. T., Kim, J. H., & Rizzino, A. (2002). Stimulation of the murine type II transforming growth factor-beta receptor promoter by the transcription factor Egr-1. Molecular Reproduction and Development, 63(3), 282-90.
Wilder PJ, et al. Stimulation of the Murine Type II Transforming Growth Factor-beta Receptor Promoter By the Transcription Factor Egr-1. Mol Reprod Dev. 2002;63(3):282-90. PubMed PMID: 12237943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stimulation of the murine type II transforming growth factor-beta receptor promoter by the transcription factor Egr-1. AU - Wilder,Phillip J, AU - Bernadt,Cory T, AU - Kim,Jae-Hwan, AU - Rizzino,Angie, PY - 2002/9/19/pubmed PY - 2003/3/11/medline PY - 2002/9/19/entrez SP - 282 EP - 90 JF - Molecular reproduction and development JO - Mol Reprod Dev VL - 63 IS - 3 N2 - Previous studies have demonstrated that differentiation of murine embryonal carcinoma (EC) cells leads to the appearance of high affinity receptors for transforming growth factor-beta (TGF-beta). Subsequently, it was demonstrated that differentiation of F9 EC cells leads to increases in the transcription of the type II TGF-beta-receptor gene (TbetaR-II) and leads to significant increases in the steady-state levels of TbetaR-II mRNA. Analysis of the human TbetaR-II promoter in F9-differentiated cells identified several cis-regulatory elements that influence the activity of the promoter, including a CRE/ATF site and a CCAAT box motif. In the work described in this report, we focused on the effect of the transcription factor Egr-1 on the murine TbetaR-II promoter. We have identified an Egr-1 response-element approximately 150 bp upstream of the major transcription start site of the murine TbetaR-II gene. We demonstrate by electrophoretic mobility shift analysis (EMSA) that this cis-regulatory element binds Egr-1, and we demonstrate that disruption of this site eliminates the response to Egr-1. As part of this analysis, we also examined the effect of Egr-1 on human TbetaR-II promoter. In contrast to a previous report, which reported that Egr-1 inhibits expression of human TbetaR-II promoter/reporter gene constructs, we did not observe an inhibitory effect of Egr-1 that was specific for the human TbetaR-II promoter. Taken together, the findings described in this report identify important differences between the human and the murine TbetaR-II promoter, and our findings identify an Egr-1 cis-regulatory element that is capable of stimulating the activity of the murine TbetaR-II promoter. SN - 1040-452X UR - https://www.unboundmedicine.com/medline/citation/12237943/Stimulation_of_the_murine_type_II_transforming_growth_factor_beta_receptor_promoter_by_the_transcription_factor_Egr_1_ L2 - https://doi.org/10.1002/mrd.10165 DB - PRIME DP - Unbound Medicine ER -