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TWIST inactivation reduces CBFA1/RUNX2 expression and DNA binding to the osteocalcin promoter in osteoblasts.
Biochem Biophys Res Commun. 2002 Sep 27; 297(3):641-4.BB

Abstract

The Saethre-Chotzen (SC) syndrome is characterized by increased osteogenesis and premature fusion of cranial sutures, resulting from mutations in TWIST, a basic helix-loop-helix transcription factor. The molecular target genes for Twist in osteoblasts are however unknown. We report here that TWIST haploinsufficiency in mutant osteoblasts reduces mRNA and protein levels for CBFA1/RUNX2, a specific osteoblast transcription factor, during both osteoblast cell growth and in vitro osteogenesis. Moreover, this is associated with altered expression of major osteoblast-specific genes. Electrophoretic mobility shift assay (EMSA) showed reduced-binding ability of Cbfa1 to its target OSE2 element in the osteocalcin promoter in mutant osteoblasts. By contrast, TWIST inactivation does not hamper Cbfa1 binding on a similar upstream element present in the alpha1(I) collagen promoter in mutant osteoblasts. This provides the first evidence that TWIST inactivation alters CBFA1/RUNX2 expression and Cbfa1 binding ability to the osteocalcin promoter, indicating that CBFA1/RUNX2 is a target gene for TWIST in human osteoblasts.

Authors+Show Affiliations

INSERM Unit 349 Affiliated CNRS, Lariboisière Hospital, 2 rue Ambroise Paré, 75475 10, Paris Cedex, FranceNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12270142

Citation

Yousfi, Malika, et al. "TWIST Inactivation Reduces CBFA1/RUNX2 Expression and DNA Binding to the Osteocalcin Promoter in Osteoblasts." Biochemical and Biophysical Research Communications, vol. 297, no. 3, 2002, pp. 641-4.
Yousfi M, Lasmoles F, Marie PJ. TWIST inactivation reduces CBFA1/RUNX2 expression and DNA binding to the osteocalcin promoter in osteoblasts. Biochem Biophys Res Commun. 2002;297(3):641-4.
Yousfi, M., Lasmoles, F., & Marie, P. J. (2002). TWIST inactivation reduces CBFA1/RUNX2 expression and DNA binding to the osteocalcin promoter in osteoblasts. Biochemical and Biophysical Research Communications, 297(3), 641-4.
Yousfi M, Lasmoles F, Marie PJ. TWIST Inactivation Reduces CBFA1/RUNX2 Expression and DNA Binding to the Osteocalcin Promoter in Osteoblasts. Biochem Biophys Res Commun. 2002 Sep 27;297(3):641-4. PubMed PMID: 12270142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TWIST inactivation reduces CBFA1/RUNX2 expression and DNA binding to the osteocalcin promoter in osteoblasts. AU - Yousfi,Malika, AU - Lasmoles,Françoise, AU - Marie,Pierre J, PY - 2002/9/25/pubmed PY - 2002/11/26/medline PY - 2002/9/25/entrez SP - 641 EP - 4 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 297 IS - 3 N2 - The Saethre-Chotzen (SC) syndrome is characterized by increased osteogenesis and premature fusion of cranial sutures, resulting from mutations in TWIST, a basic helix-loop-helix transcription factor. The molecular target genes for Twist in osteoblasts are however unknown. We report here that TWIST haploinsufficiency in mutant osteoblasts reduces mRNA and protein levels for CBFA1/RUNX2, a specific osteoblast transcription factor, during both osteoblast cell growth and in vitro osteogenesis. Moreover, this is associated with altered expression of major osteoblast-specific genes. Electrophoretic mobility shift assay (EMSA) showed reduced-binding ability of Cbfa1 to its target OSE2 element in the osteocalcin promoter in mutant osteoblasts. By contrast, TWIST inactivation does not hamper Cbfa1 binding on a similar upstream element present in the alpha1(I) collagen promoter in mutant osteoblasts. This provides the first evidence that TWIST inactivation alters CBFA1/RUNX2 expression and Cbfa1 binding ability to the osteocalcin promoter, indicating that CBFA1/RUNX2 is a target gene for TWIST in human osteoblasts. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/12270142/TWIST_inactivation_reduces_CBFA1/RUNX2_expression_and_DNA_binding_to_the_osteocalcin_promoter_in_osteoblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X0202260X DB - PRIME DP - Unbound Medicine ER -