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Capsaicin receptor immunoreactivity in the human trigeminal ganglion.
Neurosci Lett. 2002 Sep 27; 330(3):223-6.NL

Abstract

The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization.

Authors+Show Affiliations

Department of Internal Medicine, Lund University Hospital, S-221 85 Lund, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12270633

Citation

Hou, Mingyan, et al. "Capsaicin Receptor Immunoreactivity in the Human Trigeminal Ganglion." Neuroscience Letters, vol. 330, no. 3, 2002, pp. 223-6.
Hou M, Uddman R, Tajti J, et al. Capsaicin receptor immunoreactivity in the human trigeminal ganglion. Neurosci Lett. 2002;330(3):223-6.
Hou, M., Uddman, R., Tajti, J., Kanje, M., & Edvinsson, L. (2002). Capsaicin receptor immunoreactivity in the human trigeminal ganglion. Neuroscience Letters, 330(3), 223-6.
Hou M, et al. Capsaicin Receptor Immunoreactivity in the Human Trigeminal Ganglion. Neurosci Lett. 2002 Sep 27;330(3):223-6. PubMed PMID: 12270633.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capsaicin receptor immunoreactivity in the human trigeminal ganglion. AU - Hou,Mingyan, AU - Uddman,Rolf, AU - Tajti,Janos, AU - Kanje,Martin, AU - Edvinsson,Lars, PY - 2002/9/25/pubmed PY - 2002/12/17/medline PY - 2002/9/25/entrez SP - 223 EP - 6 JF - Neuroscience letters JO - Neurosci. Lett. VL - 330 IS - 3 N2 - The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/12270633/Capsaicin_receptor_immunoreactivity_in_the_human_trigeminal_ganglion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304394002007413 DB - PRIME DP - Unbound Medicine ER -