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Inhibitory amino acid transmitters associated with axons in presynaptic apposition to cutaneous primary afferent axons in the cat spinal cord.
J Comp Neurol. 2002 Oct 14; 452(2):154-62.JC

Abstract

The purpose of the present study was to characterize the transmitter content of structures in presynaptic apposition to the central terminals of cutaneous afferent fibers in the dorsal horn of the spinal cord. Axons in the Aalphabeta conduction velocity range were identified in adult cats, stained intra-axonally with horseradish peroxidase, and prepared for combined light and electron microscopy. In total, we labeled two slowly adapting (Type 1) axons, two hair-follicle afferents, and one rapidly adapting (Krause) afferent. Ninety-nine labeled boutons were examined through complete series of serial sections. Approximately 80% of boutons originating from rapidly adapting and hair-follicle afferents were postsynaptic to other axons, but only 50% of boutons from slowly adapting axons were associated with this type of arrangement. Postembedding immunogold reactions revealed that between 80% (for slowly adapting axons) and 100% (for rapidly adapting axons) of boutons presynaptic to primary afferents were immunoreactive for gamma-aminobutyric acid (GABA). The vast majority of these terminals (in excess of 80%) were also enriched with glycine. Therefore, presynaptic inhibition of these three functional classes of Aalphabeta cutaneous primary afferents is mediated principally by the subgroup of GABAergic interneuron that also contains glycine.

Authors+Show Affiliations

Spinal Cord Group, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12271489

Citation

Sutherland, Fiona I., et al. "Inhibitory Amino Acid Transmitters Associated With Axons in Presynaptic Apposition to Cutaneous Primary Afferent Axons in the Cat Spinal Cord." The Journal of Comparative Neurology, vol. 452, no. 2, 2002, pp. 154-62.
Sutherland FI, Bannatyne BA, Kerr R, et al. Inhibitory amino acid transmitters associated with axons in presynaptic apposition to cutaneous primary afferent axons in the cat spinal cord. J Comp Neurol. 2002;452(2):154-62.
Sutherland, F. I., Bannatyne, B. A., Kerr, R., Riddell, J. S., & Maxwell, D. J. (2002). Inhibitory amino acid transmitters associated with axons in presynaptic apposition to cutaneous primary afferent axons in the cat spinal cord. The Journal of Comparative Neurology, 452(2), 154-62.
Sutherland FI, et al. Inhibitory Amino Acid Transmitters Associated With Axons in Presynaptic Apposition to Cutaneous Primary Afferent Axons in the Cat Spinal Cord. J Comp Neurol. 2002 Oct 14;452(2):154-62. PubMed PMID: 12271489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory amino acid transmitters associated with axons in presynaptic apposition to cutaneous primary afferent axons in the cat spinal cord. AU - Sutherland,Fiona I, AU - Bannatyne,B Anne, AU - Kerr,Robert, AU - Riddell,John S, AU - Maxwell,David J, PY - 2002/9/25/pubmed PY - 2002/11/1/medline PY - 2002/9/25/entrez SP - 154 EP - 62 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 452 IS - 2 N2 - The purpose of the present study was to characterize the transmitter content of structures in presynaptic apposition to the central terminals of cutaneous afferent fibers in the dorsal horn of the spinal cord. Axons in the Aalphabeta conduction velocity range were identified in adult cats, stained intra-axonally with horseradish peroxidase, and prepared for combined light and electron microscopy. In total, we labeled two slowly adapting (Type 1) axons, two hair-follicle afferents, and one rapidly adapting (Krause) afferent. Ninety-nine labeled boutons were examined through complete series of serial sections. Approximately 80% of boutons originating from rapidly adapting and hair-follicle afferents were postsynaptic to other axons, but only 50% of boutons from slowly adapting axons were associated with this type of arrangement. Postembedding immunogold reactions revealed that between 80% (for slowly adapting axons) and 100% (for rapidly adapting axons) of boutons presynaptic to primary afferents were immunoreactive for gamma-aminobutyric acid (GABA). The vast majority of these terminals (in excess of 80%) were also enriched with glycine. Therefore, presynaptic inhibition of these three functional classes of Aalphabeta cutaneous primary afferents is mediated principally by the subgroup of GABAergic interneuron that also contains glycine. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/12271489/Inhibitory_amino_acid_transmitters_associated_with_axons_in_presynaptic_apposition_to_cutaneous_primary_afferent_axons_in_the_cat_spinal_cord_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=2002&volume=452&issue=2&spage=154 DB - PRIME DP - Unbound Medicine ER -