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Light and electron microscopic analysis of aquaporin 1-like-immunoreactive amacrine cells in the rat retina.
J Comp Neurol 2002; 452(2):178-91JC

Abstract

Aquaporin 1 (AQP1; also known as CHIP, a channel-forming integral membrane protein of 28 kDa) is the first protein to be shown to function as a water channel and has been recently shown to be present in the rat retina. We previously showed (Kim et al. [1998] Neurosci Lett 244:52-54) that AQP1-like immunoreactivity is present in a certain population of amacrine cells in the rat retina. This study was conducted to characterize these cells in more detail. With immunocytochemistry using specific antisera against AQP1, whole-mount preparations and 50-microm-thick vibratome sections were examined by light and electron microscopy. These cells were a class of amacrine cells, which had symmetric bistratified dendritic trees ramified in stratum 2 and in the border of strata 3 and 4 of the inner plexiform layer (IPL). Their dendritic field diameters ranged from 90 to 230 microm. Double labeling with antisera against AQP1 and gamma-aminobutyric acid or glycine demonstrated that these AQP1-like-immunoreactive amacrine cells were immunoreactive for glycine. Their most frequent synaptic input was from other amacrine cell processes in both sublaminae a and b of the IPL, followed by a few cone bipolar cells. Their primary targets were other amacrine cells and ganglion cells in both sublaminae a and b of the IPL. In addition, synaptic output onto bipolar cells was rarely observed in sublamina b of the IPL. Thus, the AQP1 antibody labels a class of glycinergic amacrine cells with small to medium-sized dendritic fields in the rat retina.

Authors+Show Affiliations

Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12271491

Citation

Kim, In-Beom, et al. "Light and Electron Microscopic Analysis of Aquaporin 1-like-immunoreactive Amacrine Cells in the Rat Retina." The Journal of Comparative Neurology, vol. 452, no. 2, 2002, pp. 178-91.
Kim IB, Lee EJ, Oh SJ, et al. Light and electron microscopic analysis of aquaporin 1-like-immunoreactive amacrine cells in the rat retina. J Comp Neurol. 2002;452(2):178-91.
Kim, I. B., Lee, E. J., Oh, S. J., Park, C. B., Pow, D. V., & Chun, M. H. (2002). Light and electron microscopic analysis of aquaporin 1-like-immunoreactive amacrine cells in the rat retina. The Journal of Comparative Neurology, 452(2), pp. 178-91.
Kim IB, et al. Light and Electron Microscopic Analysis of Aquaporin 1-like-immunoreactive Amacrine Cells in the Rat Retina. J Comp Neurol. 2002 Oct 14;452(2):178-91. PubMed PMID: 12271491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light and electron microscopic analysis of aquaporin 1-like-immunoreactive amacrine cells in the rat retina. AU - Kim,In-Beom, AU - Lee,Eun-Jin, AU - Oh,Su-Ja, AU - Park,Cheol-Beom, AU - Pow,David V, AU - Chun,Myung-Hoon, PY - 2002/9/25/pubmed PY - 2002/11/1/medline PY - 2002/9/25/entrez SP - 178 EP - 91 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 452 IS - 2 N2 - Aquaporin 1 (AQP1; also known as CHIP, a channel-forming integral membrane protein of 28 kDa) is the first protein to be shown to function as a water channel and has been recently shown to be present in the rat retina. We previously showed (Kim et al. [1998] Neurosci Lett 244:52-54) that AQP1-like immunoreactivity is present in a certain population of amacrine cells in the rat retina. This study was conducted to characterize these cells in more detail. With immunocytochemistry using specific antisera against AQP1, whole-mount preparations and 50-microm-thick vibratome sections were examined by light and electron microscopy. These cells were a class of amacrine cells, which had symmetric bistratified dendritic trees ramified in stratum 2 and in the border of strata 3 and 4 of the inner plexiform layer (IPL). Their dendritic field diameters ranged from 90 to 230 microm. Double labeling with antisera against AQP1 and gamma-aminobutyric acid or glycine demonstrated that these AQP1-like-immunoreactive amacrine cells were immunoreactive for glycine. Their most frequent synaptic input was from other amacrine cell processes in both sublaminae a and b of the IPL, followed by a few cone bipolar cells. Their primary targets were other amacrine cells and ganglion cells in both sublaminae a and b of the IPL. In addition, synaptic output onto bipolar cells was rarely observed in sublamina b of the IPL. Thus, the AQP1 antibody labels a class of glycinergic amacrine cells with small to medium-sized dendritic fields in the rat retina. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/12271491/Light_and_electron_microscopic_analysis_of_aquaporin_1_like_immunoreactive_amacrine_cells_in_the_rat_retina_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=2002&volume=452&issue=2&spage=178 DB - PRIME DP - Unbound Medicine ER -